PE/Cyanine7 anti-mouse CD4 100528 from BioLegend

Product Specs
ItemPE/Cyanine7 anti-mouse CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100528
NCBI Gene AliasesL3T4, Ly-4
Conjugate/TagPE_CYANINE7
IsotypeIgG2a, Kappa
Quantity100 µg
CloneRM4-5
HostRat
ReactivityMouse
ApplicationsFlow Cytometry (FC)
NCBI Full Gene NameCD4 antigen
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenBALB/c mouse thymocytes
Antibody TypePrimary
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Acute irradiation causes a long-term disturbance in the heterogeneity and gene expression profile of medullary thymic epithelial cellsFrontiers in ImmunologyNovember 2, 2023Kenta Horie, Kano Namiki, Kyouhei Kinoshita, Maki Miyauchi, Tatsuya Ishikawa, Mio Hayama, Yuya Maruyama, Naho Hagiwara, Takahisa Miyao, Shigeo Murata, et al.PECy7-streptavidin (eBioscience, Cat#25-4317-82), PECy7-anti-mouse CD4 (BioLegend, clone RM4-5, Cat#100528), APCCy7-anti-mouse CD8 (BioLegend, clone 53-6.7, Cat#109220), PE-anti-mouse CD44 (BioLegend, clone

(2) Lung cDC1 and cDC2 dendritic cells priming naive CD8+ T cells in situ prior to migration to draining lymph nodesCell ReportsOctober 31, 2023Youhui Si, Yihan Wang, Qiaomu Tian, Qiang Wang, Jared M. Pollard, Pramod K. Srivastava, Aaron P. Esser-Kahn, Joel H. Collier, Anne I. Sperling, Anita S. Chong16-10A1,BioLegend,Cat# 104737; RRID: AB_2564174 anti-mouse CD4 (PE/Cyanine7); Clone RM4-5,BioLegend,Cat# 100527; RRID: AB_312728 anti-mouse CD8a (BV510); Clone 53-6.7,BioLegend,Cat# 100751; RRID: AB_2561389 anti-mouse

(3) Tumors produce glucocorticoids by metabolite recycling, not synthesis, and activate Tregs to promote growthJournal of Clinical InvestigationSeptember 15, 2023Matthew D. Taves, Shizuka Otsuka, Michaela A. Taylor, Kaitlynn M. Donahue, Thomas J. Meyer, Margaret C. Cam, Jonathan D. AshwellBD Biosciences); Alexa Fluor 700–conjugated TCR-β chain (H57-597, 109224), PE/Cy7-conjugated anti-CD4 (RM4-5, 100527), Alexa Fluor 647–conjugated anti-CD39 (Duha59, 143808), Brilliant Violet 421–conjugated anti-CD73 (TY/11.8,

(4) METTL3 drives NAFLD-related hepatocellular carcinoma and is a therapeutic target for boosting immunotherapyCell Rep MedAugust 15, 2023Yasi Pan, Huarong Chen, Xiang Zhang, Weixin Liu, Yanqiang Ding, Dan Huang, Jianning Zhai, Wenchao Wei, Jun Wen, Danyu Chen, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-mouse CD4 (PE-Cy7) (RM4-5),Biolegend,Cat# 100528; RRID: AB_312729 Anti-mouse CD279(BV421) (29F.1A12),Biolegend,Cat# 135221; RRID: AB_2562568 Anti-mouse

(5) Combining gut microbiota modulation and chemotherapy by capecitabine-loaded prebiotic nanoparticle improves colorectal cancer therapyNat CommunAugust 7, 2023Tianqun Lang, Runqi Zhu, Xiao Zhu, Wenlu Yan, Yu Li, Yihui Zhai, Ting Wu, Xin Huang, Qi Yin, Yaping Lihttps://cytekbio.com/products/percp-cyanine5-5-anti-mouse-cd3e-145-2c11), PE-Cy7 anti-mouse CD4 (BioLegend, cat. 100527, Clone: RM4-5, 1:100 dilution, https://www.biolegend.com/en-us/products/pe-cyanine7-anti-mouse-cd4-antibody-1932),

(6) Potentiating the Cross-Reactive IFN-γ T Cell and Polyfunctional T Cell Responses by Heterologous GX-19N DNA Booster in Mice Primed with Either a COVID-19 mRNA Vaccine or Inactivated VaccineInternational Journal of Molecular SciencesJune 5, 2023Yong Bok Seo, Ara Ko, Duckhyang Shin, Junyoung Kim, You Suk Suh, Juyoung Na, Ji In Ryu, Suyeon Lee, Min Ji Oh, Young Chul SungCD8 FITC (Biolegend, 100706, San Diego, CA, USA), IL-2 PE (Biolegend 503808), CD4 PE-Cy7 (Biolegend 100528), IFN-γ APC (Biolegend 505810), TNF-α (Biolegend 506328), CD3 BV605 (Biolgend 100351), and Live/dead

(7) T cell‐dependent bispecific antibodies alter organ‐specific endothelial cell–T cell interactionEMBO reportsMarch 6, 2023Patricia Himmels, Thi Thu Thao Nguyen, Maresa Caunt Mitzner, Alfonso Arrazate, Stacey Yeung, Jeremy Burton, Robyn Clark, Klara Totpal, Raj Jesudason, Angela Yang, et al.CD4‐BV650, clone GK1.5 (RUO)",BD Biosciences,563232 "Rat anti‐mouse CD4‐PE/Cyanine7, clone RM4‐5",Biolegend,100528 "Rat anti‐mouse CD45‐BUV395, clone 30‐F11 (RUO)",BD Biosciences,564279 "Rat anti‐mouse CD8a‐BV711,

(8) Topical Diacerein Decreases Skin and Splenic CD11c+ Dendritic Cells in PsoriasisInternational Journal of Molecular SciencesFebruary 21, 2023Susanne M. Brunner, Andrea Ramspacher, Caroline Rieser, Julia Leitner, Hannah Heil, Michael Ablinger, Julia Tevini, Monika Wimmer, Andreas Koller, Josefina Piñón Hofbauer, et al.Horizon,564144,BUV395,1:100 CD19,6D5,BioLegend,115541,BV650,1:100 CD3ε,145-2C11,BioLegend,100305,FITC,1:100 F4/80,T45-2342,BD Horizon,565612,BV711,1:100 Ly-6G/Ly-6C,RB6-8C5,BioLegend,108407,PE,1:100 CD4,RM4-5,BioLegend,100528,PE/Cy7,1:300 CD8a,53-6.7,eBioscience,17-0081-82,APC,1:400 CD11b,M1/70,Invitrogen,56-0112-82,AF700,1:100 CD11c,N418,eBioscience,48-0114-82,eFluor450,1:100 CD45,30-F11,Invitrogen,45-0451-82,PerCP-Cy5,1:1600 Viability

(9) Epigenetically suppressed tumor cell intrinsic STING promotes tumor immune escapeBiomedicine & PharmacotherapyJanuary 1, 2023Hui Zheng, Lizhen Wu, Qian Xiao, Xin Meng, Alex Hafiz, Qin Yan, Renquan Lu, Jian Cao(RM4–5)PE (eBioscience, #12–0042-82, clone RM4–5), PE/Cyanine7 anti-mouse CD4 Antibody (BioLegend, #100527, clone RM4–5), PE/Cyanine7 anti-mouse CD45.2 Antibody (BioLegend, # 109829, Clone 104), PE anti-mouse CD69

(10) CLEC5a-directed bispecific antibody for effective cellular phagocytosismAbsDecember 31, 2022Vivekananda Kedage et al.anti-CD25 antibody (ThermoFisher scientific, catalog 53–0251-82), anti-CD4 antibody (BioLegend, catalog 100528), anti-CD44 antibody (ThermoFisher scientific, catalog 17–0441-83), anti-CD8b antibody (BD Biosciences,

(11) Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programmingSTAR ProtocolsDecember 16, 2022Miriam A. Lynn, Feargal J. Ryan, Yee Chern Tee, David J. Lynn(clone 53-6.7), 200×",BD Biosciences,Cat#557654; RRID:AB_396769 "CD4 (clone RM4-5), 400×",BioLegend,Cat#100527; RRID:AB_312728 "CD3 (145-2C11), 200×",BD Biosciences,Cat#562600; RRID:AB_11153670 "CD44 (Clone IM7),

(12) A microarray patch SARS-CoV-2 vaccine induces sustained antibody responses and polyfunctional cellular immunityiScienceOctober 21, 2022Balmert SC, Ghozloujeh ZG, Carey CD, Williams LH, Zhang J, Shahi P, Amer M, Sumpter TL, Erdos G, Korkmaz E, et al.BUV395,BD Biosciences,Cat# 563790; RRID: AB_2738426 Anti-mouse CD4 (clone RM4-5) PE-Cy7,BioLegend,Cat# 100528; RRID: AB_312729 Anti-mouse CD8b (clone H35-17.2) BUV737,BD Biosciences,Cat# 741811; RRID: AB_2871149 Anti-mouse

(13) Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunityCancer cellOctober 10, 2022Chryplewicz A, Scotton J, Tichet M, Zomer A, Shchors K, Joyce JA, Homicsko K, Hanahan D.GK1.5),Biolegend,Cat#: 100404; RRID: AB_312689 Pe-Cyanine7 CD4 anti-mouse (clone RM4-5),Biolegend,Cat#: 100528; RRID: AB_312729 Brilliant Violet 510™ CD11b anti-mouse/human (clone M1/70),Biolegend,Cat#: 101245;

(14) DPP9 deficiency: an Inflammasomopathy which can be rescued by lowering NLRP1/IL-1 signalingScience ImmunologySeptember 16, 2022Cassandra R. Harapas, Kim S. Robinson, Kenneth Lay, Jasmine Wong, Ricardo Moreno Moreno Traspas, Nasrin Nabavizadeh, Annick Rass-Rothschild, Bertrand Boisson, Scott B. Drutman, Pawat Laohamonthonkul, et al.antibodies: MHCII-FITC (1:200 in house), CD11c-PE (1:200 BioLegend #117307), CD4-PE-Cy7 (1:200 BioLegend #100528), CD3-PE-CF594 (1:200 BD Bioscience #562286), Ly6G-APC (1:200 in house), CD19-AF700 (1:200 BioLegend

(15) Parvimonas micra promotes colorectal tumorigenesis and is associated with prognosis of colorectal cancer patientsOncogeneSeptember 2, 2022Liuyang Zhao, Xiang Zhang, Yunfei Zhou, Kaili Fu, Harry Cheuk-Hay Lau, Tommy Wai-Yiu Chun, Alvin Ho-Kwan Cheung, Olabisi Oluwabukola Coker, Hong Wei, William Ka-Kei Wu, et al.staining with surface markers CD45 (103139, Biolegend, San Diego, CA), CD3 (100205, Biolegend), CD4 (100528, Biolegend), intracellular markers Il-17 (506903, Biolegend) and interferon (IFN)-γ (505806, Biolegend). CD4+

(16) Group 3 innate lymphocytes make a distinct contribution to type 17 immunity in bladder defenceiScienceJuly 15, 2022Riding AM, Loudon KW, Guo A, Ferdinand JR, Lok LSC, Richoz N, Stewart A, Castro-Dopico T, Tuong ZK, Fiancette R, et al.(PerCP-Cy5.5),Biolegend,Cat#134320; Clone MAR; RRID:AB_10641135 anti-mouse CD4 (PE-Cyanine7),Biolegend,Cat#100528; Clone GK1.5; RRID:AB_312729 anti-mouse γδTCR (PE-Cyanine7),Biolgend,Cat#118124; Clone GL3; RRID:AB_11204423 anti-mouse

(17) T-cell Dysfunction upon Expression of MYC with Altered Phosphorylation at Threonine 58 and Serine 62Molecular Cancer ResearchJuly 6, 2022Colin J. Daniel, Carl Pelz, Xiaoyan Wang, Michael W. Munks, Aaron Ko, Dhaarini Murugan, Sarah A. Byers, Eleonora Juarez, Karyn L. Taylor, Guang Fan, et al.(H57–597, BD Biosciences, catalog no. 612821, RRID:AB_2870145), CD4-PE/Cy7 (RM4–5, BioLegend, catalog no. 100528, RRID:AB_312729), CD8-PE/Cy7 (53–6.7, BioLegend, catalog no. 100722, RRID:AB_312761), CD19-BV650 (6D5,

(18) Host lung microbiota promotes malaria-associated acute respiratory distress syndromeNature communicationsJune 29, 2022Mukherjee D, Chora ÂF, Lone JC, Ramiro RS, Blankenhaus B, Serre K, Ramirez M, Gordo I, Veldhoen M, Varga-Weisz P, et al.distribution of CD4+ and CD8+ cells, using anti-CD4 PeCy7 (clone RM4-5, BioLegend, catalogue number: 100528) and anti-CD8 FITC (clone 53-6.7, catalogue number: 100706) antibodies. 50–60% and 40–50% of the cells

(19) Reversing insufficient photothermal therapy-induced tumor relapse and metastasis by regulating cancer-associated fibroblastsNature communicationsMay 19, 2022Li X, Yong T, Wei Z, Bie N, Zhang X, Zhan G, Li J, Qin J, Yu J, Zhang B, et al.1/200 dilution), CD3 (Biolegend, cat. No 100218, clone 17A2, 1/20 dilution), CD4 (Biolegend, cat. No 100528, clone RM4-5, 1/80 dilution), CD8 (Biolegend, cat. No 100712, clone 53–6.7, 1/80 dilution), CD69 (Biolegend,

(20) Molecular profiling reveals features of clinical immunity and immunosuppression in asymptomatic P. falciparum malariaMolecular Systems BiologyApril 27, 2022Stephanie I Studniberg et al.,BD Biosciences,Clone H57‐597; Cat# 553170 PE/Cy7‐conjugated rat anti‐mouse CD4,Biolegend,Clone RM4‐5; Cat# 100528 BV785‐conjugated anti‐mouse CD44,Biolegend,Clone IM7; Cat# 103041 PerCp‐Cy5.5‐conjugated rat anti‐mouse

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(1)

8/26/2021Very Bright Fluorophore and Good Antibody Owen JiangReliable Antibody for flow analysis. Try 1:100 dilution in FACS buffer first. Titration might be needed for optimized results.
(1)

4/29/2015Very Useful CD4 Antibody Ferdinando PucciThe aim of my research is to study lymph node cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters cell counts. We selected CD4 Antibody from Biolegend ... Read Review

PE/Cyanine7 anti-mouse CD4 from BioLegend

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PE/Cyanine7 anti-mouse CD4 from BioLegend

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