Anti-ABCA1 antibody ab7360 from Abcam

Product Specs
ItemAnti-ABCA1 antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab7360
Antibody TypePrimary
ClonalityPolyclonal
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
HostRabbit
Quantity100 UL
IsotypeIgG
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaNeuroscience
Gene NameABCA1
ReactivityHuman, Mouse
TargetABCA1

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Loss of Lipid Carrier ApoE Exacerbates Brain Glial and Inflammatory Responses after Lysosomal GBA1 InhibitionCellsNovember 2, 2023Kyle J. Connolly, Juliette Margaria, Erika Di Biase, Oliver Cooper, Penelope J. Hallett, Ole Isacsonprobed overnight at 4 °C with primary antibodies against the following target proteins: ABCA1 (Abcam, ab7360, 1:1000), ApoE (Abcam, ab183597, 1:1000), C1q (Abcam, ab182451, 1:500), GFAP (EMD Millipore, MAB360,

(2) Atheroprotective Effect of Fucoidan in THP-1 Macrophages by Potential Upregulation of ABCA1BiomedicinesOctober 30, 2023Zeenat Mirza, Dalal A. Al-Saedi, Salma Saddeek, Sanaa Almowallad, Rehab F. AlMassabi, Etimad Huwaitantibody for 1 h (β-actin, 1/2000, Thermo Fisher Scientific, PA1-183) or overnight (ABCA1, 1/1000, Abcam, ab7360). It was further washed thrice for 10 min each with TBS-T, then the membrane was immersed in goat anti-rabbit

(3) Interactions between Angiotensin Type-1 Antagonists, Statins, and ROCK Inhibitors in a Rat Model of L-DOPA-Induced DyskinesiaAntioxidants (Basel)July 19, 2023Andrea Lopez-Lopez, Rita Valenzuela, Ana Isabel Rodriguez-Perez, María J. Guerra, Jose Luis Labandeira-Garcia, Ana Muñozovernight with primary antibodies against proteins of interest: rabbit polyclonal anti-ABCA1 transporter (ab7360; 1:1000, Abcam, Cambridge, UK), rabbit polyclonal anti-LDL receptor, LDLR (ab30532; 1:1000, Abcam),

(4) Initial Characterization of 3D Culture of Yolk Sac TissueAnimals (Basel)April 22, 2023Vitória Mattos Pereira, Priscila Avelino Ferreira Pinto, Lina Castelo Branco Motta, Matheus F. Almeida, André Furugen Cesar de Andrade, Ana Paula Pinoti Pavaneli, Carlos Eduardo Ambrósiocholesterol and minerals, respectively, in the YS membrane [29]. The antibodies used were anti-ABCA1 (ab7360, Abcam) and anti-SLC39A7 (ZIP7, ab117560; Abcam) at a dilution of 1:500 for anti-ABCA1 and 5 μg/mL for

(5) Manipulation of tissue factor-mediated basal PAR-2 signalling on macrophages determines sensitivity for IFNγ responsiveness and significantly modifies the phenotype of murine DTHFrontiers in ImmunologySeptember 12, 2022Hannah Wilkinson, Hugh Leonard, Michael G. Robson, Richard Smith, ElLi Tam, John H. McVey, Daniel Kirckhofer, Daxin Chen, Anthony Dorling(ab5690 Abcam, Cambridge, UK) and rabbit anti mouse CD206 (ab64693 Abcam), iNOS (ab15323 Abcam), ABCA1 (ab7360 Abcam), IL-10 (ab34843 Abcam) and IFNγ (ab9657 Abcam). Primary antibodies were incubated overnight in

(6) Role and mechanism of the zinc finger protein ZNF580 in foam-cell formationExperimental and therapeutic medicineSeptember 1, 2022Zhang Z, Qin X, Liu J, Li Y, Chen H, Xie H, Chen J, Li C, Tong Y, Yang M, et al.Proteintech Group, Inc.), CD36 (cat. no. 18836-1-AP; 1:2,000 dilution; Proteintech Group, Inc.), ABCA1 (cat. no. AB7360; 1:1,000 dilution; Abcam), PPAR-γ (cat. no. 16643-1-AP; 1:2,000 dilution; Proteintech Group, Inc.) and

(7) Selective activation of ABCA1/ApoA1 signaling in the V1 by magnetoelectric stimulation ameliorates depression via regulation of synaptic plasticityiScienceMay 20, 2022Lu Q, Wu F, Jiao J, Xue L, Song R, Shi Y, Kong Y, Sun J, Gu N, Han MH, et al.probed with following appropriate antibodies according to standard procedures: ABCA1 (1:1,000, Abcam, ab7360), ApoA1 (1:1,000, Invitrogen, MIA1404), PSD-95 (1:1,000, Abcam, ab13552), Synapsin I (1:1,000, Abcam,

(8) ABCA1 and ABCG1 DNA methylation in epicardial adipose tissue of patients with coronary artery diseaseBMC Cardiovascular DisordersDecember 1, 2021Valentina V. Miroshnikova et al.pre-incubated with 5% skim milk in PBS. The blots were incubated with polyclonal rabbit anti-ABCA1 (1:1000; ab7360, Abcam, United Kingdom) and polyclonal rabbit anti-β-actin (1:5000; ab8227, Abcam, United Kingdom) diluted

(9) ALK7 Acts as a Positive Regulator of Macrophage Activation through Down-Regulation of PPARγ ExpressionJournal of atherosclerosis and thrombosisApril 1, 2021Cheng WL, Zhang Q, Cao JL, Chen XL, Li W, Zhang L, Chao SP, Zhao F.,BD Biosciences,Mouse KLF4,ab151733,Abcam,Rabbit CD36,sc-7309,Santa Cruz Biotechnology,Mouse SR-A,sc-166139,Santa Cruz Biotechnology,Mouse ABCA1,ab7360,Abcam,Rabbit ABCG1,ab244442,Abcam,Rabbit PPARγ,16643-1-AP,Proteintech,Rabbit GAPDH,2118,Cell Signaling Technology

(10) Long non‑coding RNA MALAT1 regulates cholesterol accumulation in ox‑LDL‑induced macrophages via the microRNA‑17‑5p/ABCA1 axisMolecular medicine reportsApril 1, 2020Liu L, Tan L, Yao J, Yang L.anti-apolipoprotein E (ApoE; 1:500; cat. no. A16344; ABclonal Biotech Co., Ltd.); anti-ABCA1 (1:1,000; cat. no. ab7360; Abcam); and anti-GAPDH (1:10,000; cat. no. 60004-1-Ig; ProteinTech Group, Inc.). On the next day, the

(11) Gypenosides mediate cholesterol efflux and suppress oxidized LDL induced inflammation in retinal pigment epithelium cellsExperimental eye researchFebruary 1, 2020Biswas L, Zeng Z, Graham A, Shu X.for Western blotting Antibodies Species Dilution Manufacturer Cat No. LXRα Rabbit 1:1000 Abcam ab176323 ABCA1 Rabbit 1:250 Abcam ab7360 ABCG1 Rabbit 1:1000 Abcam ab52617 CYP27A1 Rabbit 1:1000 Abcam ab64889 CYP46A1 Rabbit 1:1000 Novus NBP2-16066 TSPO Rabbit 1:2000 Abcam ab109497 LDLR Rabbit 1:500 Novus NBP1-0670955 β-ACTIN Mouse 1:2500 Novus NB600-501 GAPDH Mouse 1:2000 Santa Cruz

(12) Therapeutic implications of altered cholesterol homeostasis mediated by loss of CYP46A1 in human glioblastomaEMBO molecular medicineJanuary 9, 2020Han M, Wang S, Yang N, Wang X, Zhao W, Saed HS, Daubon T, Huang B, Chen A, Li G, et al.GAPDH was used as a loading control. The following primary antibodies were used: rabbit anti‐ABCA1 (ab7360, 1:500, Abcam), rabbit anti‐LDLR (ab52818, 1:500, Abcam), rabbit anti‐CYP46A1 (ab198889, 1:200, Abcam),

(13) Gene co-expression networks identify Trem2 and Tyrobp as major hubs in human APOE expressing mice following traumatic brain injuryNeurobiology of diseaseSeptember 1, 2017Castranio EL, Mounier A, Wolfe CM, Nam KN, Fitz NF, Letronne F, Schug J, Koldamova R, Lefterov I.transferred onto nitrocellulose membranes. Used were the following primary antibodies: Anti-ABCA1 (Ab7360, Abcam), anti-ApoE (178479, Calbiochem), anti-FYN (sc-16, Santa Cruz), and anti-CLU (sc-6419, SantaCruz). Statistical

(14) Leonurine Prevents Atherosclerosis Via Promoting the Expression of ABCA1 and ABCG1 in a Pparγ/Lxrα Signaling Pathway-Dependent MannerCellular Physiology and BiochemistryJune 19, 2017Ting Jiang, Kun Ren, Qian Chen, Heng Li, Rong Yao, Hu Hu, Yun-Cheng Lv, Guo-Jun Zhao(TBS-T) for 4 h at room temperature and then incubated with primary antibodies against ABCA1 (1:1, 000, ab7360, Abcam, Cambridge, UK), ABCG1 (1:1, 000, ab52617, Abcam, Cambridge, UK), PPARγ (1:1, 000, ab178860,

(15) RNA-seq reveals conservation of function among the yolk sacs of human, mouse, and chickenProceedings of the National Academy of SciencesJune 13, 2017Tereza Cindrova-Davies, Eric Jauniaux, Michael G. Elliot, Sungsam Gong, Graham J. Burton, D. Stephen Charnock-Jonespreviously described (76) using the following primary antibodies: anti-SLC39A7 (ZIP7; ab117560; Abcam) and anti-ABCA1 (ab7360; Abcam). Supporting Information. Additional methods are given in SI Appendix, Methods online. ACKNOWLEDGMENTS.

(16) Liver X receptor agonist treatment significantly affects phenotype and transcriptome of APOE3 and APOE4 Abca1 haplo-deficient micePloS oneJanuary 1, 2017Carter AY, Letronne F, Fitz NF, Mounier A, Wolfe CM, Nam KN, Reeves VL, Kamboh H, Lefterov I, Koldamova R.Tris-Glycine or 4–12% Bis-Tris gels. On nitrocellulose membranes ABCA1 was detected using polyclonal antibody, ab7360 (Abcam, Cambridge, MA), and APPfl with 6E10 antibody. β-Actin served as a loading control for all Western blots

(17) Cyclophilin A mediates the ox-LDL-induced activation and apoptosis of macrophages via autophagyInternational Journal of CardiologyDecember 16, 2016Zhiqiang Xue, Wei Yuan, Jing Li, Hong Zhou, Lihua Xu, Jiayi Weng, Xiaoyang Li, Xinru Zhang, Zhongqun Wang, Jinchuan Yan

(18) Chemotherapy Agents Alter Plasma Lipids in Breast Cancer Patients and Show Differential Effects on Lipid Metabolism Genes in Liver CellsPLOS ONEJanuary 25, 2016Monika Sharma, Jo Tuaine, Blair McLaren, Debra L. Waters, Katherine Black, Lynnette M. Jones, Sally P. A. McCormicknitrocellulose membrane and subject to western blotting with primary antibodies specific for ABCA1 (ab7360), HMGCR (ab174830), PPARγ (ab27649), LDLR (ab30532), LXRα (ab41902) (all from Abcam, Cambridge, England),

(19) Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXRCell reportsSeptember 22, 2015Gabitova L, Restifo D, Gorin A, Manocha K, Handorf E, Yang DH, Cai KQ, Klein-Szanto AJ, Cunningham D, Kratz LE, et al.and Immunohistochemistry experiments we used the following antibodies from Abcam: SREBP2 (ab30682), ABCA1 (ab7360), LDLR (ab30531), LXRα (ab41902), LXRβ (ab106473); from Cell Signaling: E-cadherin (#3195P), β-actin

(20) HDL and CER-001 Inverse-Dose Dependent Inhibition of Atherosclerotic Plaque Formation in apoE-/- Mice: Evidence of ABCA1 Down-RegulationPloS oneJanuary 1, 2015Claudine Tardy, Marine Goffinet, Nadia Boubekeur, Guy Cholez, Rose Ackermann, Gavin Sy, Constance Keyserling, Narendra Lalwani, John F Paolini, Jean-Louis Dasseux, Ronald Barbaras, Rudi Baronwas determined with Bradford assay and 40μg of sample were loaded on SDS-PAGE. The ABCA1 expression (ab7360 from Abcam—dilution 1/1000) was quantified using imageJ software. Calnexin expression was used as a

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9/6/2019Best Antibody for Immunodetection of ABCA1 in Human Raj KumarGood antibody for Immunodetection of ABCA1 in mammalian cells.

Anti-ABCA1 antibody from Abcam

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Anti-ABCA1 antibody from Abcam

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