Anti-Cyclin B1 antibody [V152] ab72 from Abcam

Product Specs
ItemAnti-Cyclin B1 antibody [V152]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab72
Antibody TypePrimary
IsotypeIgG1
CloneV152
Quantity10 UG, 100 UG
ClonalityMonoclonal
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration1.1 mg/ml
Research AreaEpigenetics
Gene NameCCNB1
ValidationKnockOut Validated
ReactivityHuman
TargetCyclin B1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) The clinical significance of cyclin B1 (CCNB1) in invasive breast cancer with emphasis on its contribution to lymphovascular invasion developmentBreast Cancer Research and TreatmentApril 1, 2023Abrar I. Aljohani, Michael S. Toss, Andrew R. Green, Emad A. Rakhavalidation and immunohistochemical (IHC) staining The primary mouse monoclonal anti-CCNB1 antibody (ab72, Abcam, UK)’s specificity was validated using western blot (WB) prior to staining with the IHC. MCF-7,

(2) Identification and validation of immunohistochemical marker panels to predict the prognosis of muscle invasive bladder cancerTranslational Andrology and UrologyFebruary 28, 2023Yidie Ying, Ziwei Wang, Yuxin Tan, Haotian Cao, Hongliang Gao, Zhensheng Zhang, Shuxiong Zeng, Chuanliang XuMAB-0834), GATA3 (MXB Biotechnologies, MAB-0695), YAP1 (Abcam, Cambridge, UK, ab52771), CCNB1 (Abcam, ab72), NEK2 (Absin, Shanghai, China, abs133048), p53 (MXB Biotechnologies, MAB-0674), ANLN (Abcam, ab211872),

(3) Septin 9 controls CCNB1 stabilization via APC/C(CDC20) during meiotic metaphase I/anaphase I transition in mouse oocytesCell proliferationFebruary 1, 2023Chen L, Ouyang YC, Gu LJ, Guo JN, Han ZM, Wang ZB, Hou Y, Schatten H, Sun QY.(Sigma Aldrich, Cat# F2168, RRID: AB_476967). Anti‐CCNB1 is a mouse monoclonal antibody (Abcam, Cat# ab72, RRID: AB_305751); Anti‐CDC20 is a rabbit polyclonal antibody (Abclonal Technology, Cat# A15656, RRID:

(4) Lactate receptor HCAR1 regulates neurogenesis and microglia activation after neonatal hypoxia-ischemiaeLifeAugust 9, 2022Kennedy L, Glesaaen ER, Palibrk V, Pannone M, Wang W, Al-Jabri A, Suganthan R, Meyer N, Austbø ML, Lin X, et al.of hypoxic-ischemia (data not shown). Primary antibodies were mouse anti-Cyclin B1 (1:500; Abcam, #ab72), rabbit anti-Cyclin D2 (1:1000; Cell Signaling Technology, #37413), and mouse anti-Vinculin (1:50000;

(5) The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assemblyJournal of cell scienceJanuary 15, 2022Tischer T, Yang J, Barford D.(Bethyl Laboratories, A302-480A; 1:500), Cep192 (Bethyl Laboratories, A302-324A; 1:1000), cyclin B1 (Abcam, ab72; 1:1000), His (Takara/Clontech, 631212; 1:1000), GFP (Roche, 11814460001; 1:1000), Cep57 (GeneTex, GTX115931;

(6) APC7 mediates ubiquitin signaling in constitutive heterochromatin in the developing mammalian brainMolecular cellJanuary 6, 2022Ferguson CJ, Urso O, Bodrug T, Gassaway BM, Watson ER, Prabu JR, Lara-Gonzalez P, Martinez-Chacin RC, Wu DY, Brigatti KW, et al.anti-NeuN,Millipore,"Cat# MAB377, RRID:AB_2298772" Mouse monoclonal anti-cyclin B1 (V152),Abcam,"Cat# ab72, RRID:AB_305751" Rat monoclonal anti-Ki-67 (SolA15),Thermo Fisher Scientific,"Cat# 14–5698-82, RRID:AB_10854564" Rabbit

(7) Emodin regulates cell cycle of non-small lung cancer (NSCLC) cells through hyaluronan synthase 2 (HA2)-HA-CD44/receptor for hyaluronic acid-mediated motility (RHAMM) interaction-dependent signaling pathwayCancer Cell InternationalDecember 1, 2021Mingzhu Li, Shengbo Jin, Yang Cao, Jian Xu, Shendong Zhu, Zheng LiImage Studio™ Lite (Lincoln, NE, US). Anti-cyclin A antibody (sc-271,682), Anti-Cyclin B antibody (ab72), Anti-Cyclin C antibody (ab85927), AntiCyclin D1 antibody (ab40754), Anti-Cyclin E1 antibody (ab133266),

(8) Counteraction between Astrin-PP1 and Cyclin-B-CDK1 pathways protects chromosome-microtubule attachments independent of biorientationNature communicationsDecember 1, 2021Song X, Conti D, Shrestha RL, Braun D, Draviam VM.Cells were stained with antibodies against α-Tubulin (Abcam; ab6160; 1:800 or 1:500), Cyclin B1 (Abcam; ab72; 1:1000), GFP (Roche; 1181446001; 1:800), mCherry (Thermo Scientific; M11217; 1:2000), SKAP (Atlas;

(9) Identifying the Effect of Ursolic Acid Against Triple-Negative Breast Cancer: Coupling Network Pharmacology With Experiments VerificationFrontiers in PharmacologyNovember 11, 2021Yubao Zhang, Xiaoran Ma, Huayao Li, Jing Zhuang, Fubin Feng, Lijuan Liu, Cun Liu, Changgang Sun(catalogue number ab26), rabbit anti-PLK1 (catalogue number ab189139), mouse anti-CCNB1 (catalogue number ab72), and GAPDH (catalogue number ab181602) were purchased from Abcam (United Kingdom). Cell Culture Three

(10) The natural product lapiferin inhibits cell proliferation and promotes cell apoptosis in gingival squamous cell carcinoma via P21 regulationMolecular medicine reportsJuly 1, 2021Liu L, Peng C, Ruan Y, Zhang Q.1:1,000; Abcam), cell division cycle 25C (Cdc 25C; cat. no. ab32444; 1:1,000; Abcam), cyclin B1 (cat. no. ab72; 1:1,000; Abcam), cleaved (cl)-caspase-3 (cat. no. 9664; 1:1,000; Cell Signaling Technology, Inc.),

(11) Growth hormone induces mitotic catastrophe of glomerular podocytes and contributes to proteinuriaCell death & diseaseApril 1, 2021Nishad R, Mukhi D, Singh AK, Motrapu M, Chintala K, Tammineni P, Pasupulati AK.anti-TGF-βR1 (ab31013), anti-HEY1 (ab154077), anti-p53 (ab26), anti-RhoA (ab54835), anti-cyclinB1 (ab72), anti-p21 (ab109520), anti-α-Tubulin (ab7291), anti-CDK4 (ab137675), anti-Ki67 (ab16667), anti-cyclinD1

(12) Human ORC/MCM density is low in active genes and correlates with replication time but does not delimit initiation zoneseLifeMarch 8, 2021Nina Kirstein et al.A1/A2, cyclin B1), or 12.5–15% gradient gel (H3S10P). Cyclin A1/A2 (Abcam, ab185619), cyclin B1 (Abcam, ab72), and H3S10P (Cell Signaling, D2C8) antibodies were used in 1:1000 dilutions, and GAPDH (clone GAPDH3

(13) Silencing CDCA8 Suppresses Hepatocellular Carcinoma Growth and Stemness via Restoration of ATF3 Tumor Suppressor and Inactivation of AKT/β-Catenin SignalingCancersMarch 2, 2021Jeon T, Ko MJ, Seo YR, Jung SJ, Seo D, Park SY, Park KU, Kim KS, Kim M, Seo JH, et al.β-actin (sc-4778), GSK-3β (sc-81462) (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA), Cyclin B1 (ab72), Bax (ab32503), Akt (ab126811), CDCA8 (ab70910, Abcam, Cambridge, UK), p-Akt(T308) (#13038), p-GSK-3β(S9)

(14) CDCA5 promotes the progression of prostate cancer by affecting the ERK signalling pathwayOncology ReportsJanuary 4, 2021Junpeng Ji, Tianyu Shen, Yang Li, Yixi Liu, Zhiqun Shang, Yuanjie Niufor western blotting" ,,1:100 dilution for IHC CDK1,"ab131450, Abcam",1:100 dilution for western blotting Cyclin B1,"ab72, Abcam","1:1,000 dilution for western blotting" GAPDH,"KM9002, Tianjin Sungene Biotech Co, Ltd.","1:5,000

(15) Echinoderm Microtubule Associated Protein Like 1 Is Indispensable for Oocyte Spindle Assembly and Meiotic Progression in MiceFrontiers in cell and developmental biologyJanuary 1, 2021Yin H, Zhang T, Wang H, Hu X, Hou X, Fang X, Yin Y, Li H, Shi L, Su YQ.polyclonal anti-tRFP/mKate (1:500, evrogen, #AB234), mouse monoclonal anti-Cyclin B1 (1:100, abcam, #ab72), mouse monoclonal anti-Cdk1/Cdk2 (1:100, Santa Cruz, #sc-53219), mouse monoclonal anti-NUDC (1:100,

(16) Cell Division Cycle 5-Like Regulates Metaphase-to-Anaphase Transition in Meiotic OocyteFrontiers in cell and developmental biologyJanuary 1, 2021Zhang HY, Li J, Ouyang YC, Meng TG, Zhang CH, Yue W, Sun QY, Qian WP.monoclonal anti-Myc antibody (Sigma, M4439, 1:3,000), mouse monoclonal anti-Cyclin B1 antibody (Abcam, ab72, 1:1,000), and anti-SMC3 antibody (Abcam, ab128919, 1:1,000) were used for Western blotting. Immunofluorescence Analysis

(17) Changes in subcellular structures and states of pumilio 1 regulate the translation of target Mad2 and cyclin B1 mRNAsJournal of cell scienceDecember 11, 2020Takei N, Takada Y, Kawamura S, Sato K, Saitoh A, Bormann J, Yuen WS, Carroll J, Kotani T.sc-752; 1:100 dilution), an anti-hamster cyclin B1 mouse monoclonal antibody (V152, Abcam, cat. no. ab72; 1:1000 dilution), and an anti-human Mad2 rabbit antibody (Bethyl Laboratories, Inc., cat. no. A300-301A;

(18) RNA demethylase ALKBH5 prevents pancreatic cancer progression by posttranscriptional activation of PER1 in an m6A-YTHDF2-dependent mannerMolecular CancerDecember 1, 2020Xingya Guo, Kai Li, Weiliang Jiang, Yangyang Hu, Wenqin Xiao, Yinshi Huang, Yun Feng, Qin Pan, Rong Wanab1431, Abcam), mouse monoclonal anti-P21 (sc-71,811, Santa Cruz), mouse monoclonal anti-CYCLIN B1 (ab72, Abcam), rabbit polyclonal anti-p-CDK1 (Tyr15; ab47594), mouse monoclonal anti-CDK1 (A17, Abcam) and

(19) Targeting the oncogene LSF with either the small molecule inhibitor FQI1 or siRNA causes mitotic delays with unaligned chromosomes, resulting in cell death or senescenceBMC CancerDecember 1, 2020Jennifer L. S. Willoughby et al.Primary antibodies included Aurora Kinase B (Abcam AB2254), Cdc20 (Abcam AB26483), Cyclin B1 (Abcam AB72), LBP-1A (Millipore Sigma ABE181), LSF (Millipore Sigma ABE180), phosphorylated Histone 3 Serine 10

(20) Particulate Matter Exposure During Oocyte Maturation: Cell Cycle Arrest, ROS Generation, and Early Apoptosis in MiceFrontiers in Cell and Developmental BiologyNovember 26, 2020Yu-Jin Jo et al.0.25% Tween 20 (TBST) and 5% BSA for 1 h, rinsed in TBST, and probed with an anti-cyclin B1 (1:1,000; #ab72; Abcam, Cambridge, United Kingdom), anti-CDK1 (1:500; #ab18; Abcam), and anti-α-tubulin (1:2000; #T5168;

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Anti-Cyclin B1 antibody [V152] from Abcam

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Anti-Cyclin B1 antibody [V152] from Abcam

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