Anti-p38 (phospho T180 + Y182) antibody ab4822 from Abcam

Product Specs
ItemAnti-p38 (phospho T180 + Y182) antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab4822
Antibody TypePrimary
ClonalityPolyclonal
HostRabbit
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
IsotypeIgG
Quantity50 UL
Conjugate/TagUnconjugated
FormatPurified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p38, and ii) a JNK-derived peptide that is phosphorylated at threonine 183 and tyrosine 185. The final product is generated by affinity chromatography using a p38-derived peptide that is phosphorylated at threonine 180 and tyrosine 182
Concentration0.125 mg/ml
ModificationsPhospho-Y182
Gene NameMAPK14
ReactivityHuman, Rat
Targetp38

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Website: http://www.abcam.com

(1) Acupuncture Relieves Cervical Spondylosis Radiculopathy by Regulating Spinal Microglia Activation Through MAPK Signaling Pathway in RatsJournal of Pain ResearchNovember 20, 2023Tianyu Shi, Yitian Liu, Bo Ji, Jiajia Wang, Yunpeng Ge, Yang Fang, Yana Xie, Hongli Xiao, Le Wu, Yifei Wangprimary antibodies were used for Western blotting: Rabbit Anti-p38 (phospho T180 + Y182) antibody (ab4822, 1:1000, Abcam), rabbit p38 MAPK Polyclonal antibody (14064-1-AP, 1:1000, Proteintech, IL, USA), Rabbit

(2) Pharmacological inhibition of protein S-palmitoylation suppresses osteoclastogenesis and ameliorates ovariectomy-induced bone lossJournal of Orthopaedic TranslationSeptember 1, 2023Linghui Ma, Liwei Zhang, Zirui Liao, Chunmei Xiu, Xi Luo, Na Luo, Lei Zhang, Guangxu He, Jianquan Chen(GB11002) was obtained from Servicebio (Wuhan, Hubei, China). Rabbit antibodies specific for p-p38 (#ab4822), NFATc1 (#ab25916), and c-Fos (#ab190289) were purchased from Abcam (Waltham, MA, USA). All above primary

(3) microRNA-130b May Induce Cerebral Vasospasm after Subarachnoid Hemorrhage via Modulating Kruppel-like Factor 4Molecular and Cellular BiologyJuly 3, 2023Zewei Huang, Jiliang Hu, Jiongfu Xu, Hao Wang, Limeng Dai1:1000–1:5000, rabbit), MMP3 (ab52915, 1:1000–1:20000, rabbit), MMP9 (ab76003, 1:1000–1:20000, rabbit), p-p38 (ab4822, 1:1000, rabbit), p38 (ab170099, 1:1000–1:5000, rabbit). In addition, GAPDH (ab181602, 1:10,000, rabbit,

(4) Peroxiredoxin 3 Inhibits Cardiac Fibrosis in Mice via NOX4-P38 SignallingCell JJune 28, 2023Xiaoqi Xiong, Jun Li, Zhen Chen, Changjun Luo, Wei Wei, Bing Li, Yi Kang, Xiuhong Nong, Fen Ai, Jing Zhangincluded total (T) P38 (1:1000 dilution, #ab178867) and phosphorylated (P) P38 (1:1000 dilution, Abcam #ab4822), NADPH oxidase 4 (NOX4, 1:1000 dilution, #ab154244) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH,

(5) The angiotensin receptor neprilysin inhibitor LCZ696 attenuates renal fibrosis via ASK1/JNK/p38 MAPK-mediated apoptosis in unilateral ureteral obstructionPLOS ONEJune 13, 2023Jun Ding, Sheng Cui, Song Yu Li, Lin Yan Cui, Qi Yan Nan, Xue Jing Lin, Mei Ying Xuan, Jian Jin, Shang Guo Piao, Yu Ji Jiang, et al.examined under light microscopy. The procedure of immunostaining for JNK (#ab76572, Abcam) and P38 (#ab4822, Abcam) was similar to that for ED-1. The number of ED-1-positive cells was autocounted in 20 randomly

(6) ADAM metallopeptidase domain 10 knockdown enables podocytes to resist high glucose stimulation by inhibiting pyroptosis via MAPK pathwayExperimental and Therapeutic MedicineApril 18, 2023Chunjie Sui, Dan Zhouno. ab176640; Abcam), anti-ERK1/2 (1:1,000; cat. no. ab17942; Abcam), anti-p-p38 (1:1,000; cat. no. ab4822; Abcam), anti-p38 (1:2,000; cat. no. ab170099; Abcam), anti-p-JNK (1:5,000; cat. no. ab124956; Abcam),

(7) Enhancing cartilage repair with optimized supramolecular hydrogel-based scaffold and pulsed electromagnetic fieldBioactive MaterialsApril 1, 2023Yucong Li, Linlong Li, Ye Li, Lu Feng, Bin Wang, Ming Wang, Haixing Wang, Meiling Zhu, Yongkang Yang, Erik I. Waldorff, et al.UK), rabbit monoclonal anti-p38 (1:1000, ab170099, Abcam, UK), rabbit monoclonal anti-p-p38 (1:1000, ab4822, Abcam, UK) and mouse monoclonal anti-β-actin (1:1000, ab8226, Abcam, UK), overnight at 4 °C. Blots

(8) Anethole Pretreatment Modulates Cerebral Ischemia/Reperfusion: The Role of JNK, p38, MMP-2 and MMP-9 PathwaysPharmaceuticals (Basel)March 15, 2023Nancy S. Younis, Maged E. Mohamed(1:1000, catalogue No. ab16502), or P-JNK (1:1000, catalogue No. ab47337), or P-p38 (1:1000, catalogue No. ab4822). JNK and p38 antibodies were obtained from Abcam Biotechnology, Cambridge, MA, USA). The slides were

(9) Epigenetic Differences Arise in Endothelial Cells Responding to Cobalt-ChromiumJournal of functional biomaterialsFebruary 26, 2023da C Fernandes CJ, da Silva RAF, de Almeida GS, Ferreira MR, de Morais PB, Bezerra F, Zambuzzi WF.anti-ERK1/2 (phospho-Thr202/ Tyr204) antibody (ab214362); anti-P38 (ab7952); and anti-P38 (phospho-T180 1 Y182]) (ab4822) were obtained from Abcam (Cambridge, MA, USA). Cell culture: Human Umbilical Vein Endothelial Cells–HUVEC

(10) In-situ forming injectable GFOGER-conjugated BMSCs-laden hydrogels for osteochondral regenerationnpj Regenerative MedicineJanuary 6, 2023Mi Yeon Ha, Dae Hyeok Yang, Su Jung You, Hyun Joo Kim, Heung Jae Chun(919301, dilution ratio 1:1000, Bio Legend, CA, USA), p38 (ab31828, dilution ratio 1:1000, Abcam), pp38 (ab4822, dilution ratio 1:1000, Abcam), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; MA5-15738, dilution

(11) Levetiracetam attenuates diabetes-associated cognitive impairment and microglia polarization by suppressing neuroinflammationFrontiers in pharmacologyJanuary 1, 2023Zhang YY, Wang L, Guo H, Han TT, Chang YH, Cui XC.min to 1 h. The primary antibodies included pJNK (ab124956, Abcam) and JNK (ab179461, Abcam), p-p38 (ab4822, Abcam), p38 (14064-1-AP, Proteintech), p-p65 (ab76302, Abcam), p65 (ab16502, Abcam), MyD88 (4283S,

(12) Protective Effect of Polyphenols Purified from Mallotus oblongfolius on Ethanol-Induced Gastric Mucosal Injury by Regulating Nrf2 and MAPKs PathwaysAntioxidants (Basel)December 12, 2022Shasha Yu, Zhouwei Duan, Peng Li, Shiping Wang, Lijun Guo, Guanghua Xia, Hui XieBiotech Co., Ltd. (Shanghai, China). The antibodies (Nrf2: ab92946, Keap1: #8047S, HO-1: ab189491, p38: ab4822, p-p38: ab4822, JNK: ab179461, p-JNK: ab76572, ERK: ab184699 and p-ERK: ab201015) were purchased from

(13) Elucidating the role of circNFIB in myocardial fibrosis alleviation by endogenous sulfur dioxideBMC Cardiovasc DisordNovember 20, 2022Jia Liu, Ranran Zhang, Dahai Wang, Yi Lin, Cui Bai, Nana Nie, Shan Gao, Qiuye Zhang, Hong Chang, Chongmin Renab7778, 1:500), α-SMA (Abcam, ab5694, 1:500), β-catenin (Abcam, ab6302, 1:500), p-P38 MAPK (Abcam, ab4822, 1:500), P38 MAPK (Abcam, ab170099, 1:500) and GAPDH (Abcam, ab181602, 1:2000) diluted in PBS with 0.05%

(14) Caffeine reduces oxidative stress to protect against hyperoxia-induced lung injury via the adenosine A2A receptor/cAMP/PKA/Src/ERK1/2/p38MAPK pathwayRedox RepNovember 11, 2022Xijuan Wang, Shuai Lv, Jianwei Sun, Meihui Zhang, Lei Zhang, Yan Sun, Ziyan Zhao, Dandan Wang, Xinjing Zhao, Jiajie Zhang(anti-p-ERK1/2) (CST, #4370, USA), anti-phospho-P38 mitogen-activated protein kinases (anti-p-p38 MAPK) (Abcam, ab4822, USA), anti-cleaved caspase-3 (Bioss, bsm-33199M, China), or anti-β-actin (CST, #3700, USA) antibodies

(15) Antinociceptive properties of losmapimod in two acute pain models in rats: behavioural analysis, immunohistochemistry, dose response, and comparison with usual analgesic drugs☆BJA OpenSeptember 1, 2022Mickaël Soued, Leila Hamdi, Mouna Ben Rehouma, Jean-Xavier Mazoit, Dan Benhamou(7 μm thickness). Sections were incubated with a rabbit polyclonal anti-p38 phosphorylated antibody (ab4822, 1:250; Abcam, Cambridge, UK) and a mouse monoclonal Anti-GFAP antibody (AMAB91033, 1:500; Sigma, St.

(16) circCNN2 Accelerates Cell Proliferation and Invasion in Lung Squamous Cell Carcinoma via Regulating miR-184/E2F1 and Activating MAPK Signaling PathwayDisease MarkersAugust 16, 2022Shan Hu, Peng Cao, Kangle Kong, Peng Han, Jiaqi Yue, Yu Deng, Fan Li, Bo Zhao1 : 1000), anti-ASK1 (ab45178, 1 : 1000), anti-p38 (ab170099, 1 : 1000), anti-p-p38 (phospho T180 + Y182; ab4822, 1 : 1000), anti-Ki67 (ab15580, 1 : 1000), and the internal control GAPDH (ab181602, 1 : 1000). The

(17) Genistein improves mitochondrial function and inflammatory in rats with diabetic nephropathy via inhibiting MAPK/NF-κB pathwayActa Cirúrgica BrasileiraAugust 15, 2022Ying Li, Santao Ou, Qi Liu, Linwang Gan, Liling Zhang, Yujie Wang, Jianhua Qin, Jin Liu, Weihua Wu1:1000; Abcam; United States of America), p53 (ab26; 1:250; Abcam; United States of America), p38 MAPK (ab4822; 1:1000; Abcam; United States of America), and p65 (ab16502; 1:1000; Abcam; United States of America).

(18) CBX2 shapes chromatin accessibility promoting AML via p38 MAPK signaling pathwayMolecular cancerJune 9, 2022Del Gaudio N, Di Costanzo A, Liu NQ, Conte L, Dell'Aversana C, Bove G, Benedetti R, Montella L, Ciardiello F, Carafa V, et al.anti-KRAS (Abcam; #ab180772), anti-pERK1/2 (Cell Signaling Technology; #9101S), anti-phospho-p38 (Abcam; #ab4822), anti-p38 (Abcam; #ab170099). Primary antibodies used for CUT&RUN: anti-CBX2 (Bethyl Laboratories;

(19) Differential susceptibility to lipopolysaccharide affects the activation of toll-like-receptor 4 signaling in THP-1 cells and PMA-differentiated THP-1 cellsInnate ImmunityMay 25, 2022Young Kyu Kim, Jeong Ho Hwang, Hoon Taek LeeBiosciences), MyD88 (1:5000, ab133739, Abcam, Cambridge, UK), TRIF (1:1000, ab13810, Abcam), p-p38 (1:1000, ab4822, Abcam), p-JNK (1:5000, ab124956, Abcam), p-ERK1/2 (1:5000, ab201015, Abcam), p-NF-κB (1:1000, 3033,

(20) ARV-771 Acts as an Inducer of Cell Cycle Arrest and Apoptosis to Suppress Hepatocellular Carcinoma ProgressionFrontiers in PharmacologyMay 4, 2022Yuanfei Deng, Cuifu Yu, Lushi Chen, Xin Zhang, Qiucheng Lei, Qing Liu, Gengxi Cai, Fang Liuanti-USP14 (ab235960), anti-UCH37 (ab133508), anti-p-ERK (ab32538), anti-ERK (ab184699), anti-p-p38 (ab4822), and anti-p38 (ab170099) were obtained from Abcam (Cambridge, MA). Anti-PARP (#9532), anti-USP1 (#8033),

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9/18/2019Average Antibody to Detect p-p38 MAPK in Human Cell Line Raj KumarThe indicated antibody has detected a specific band of p38 (phospho T180 + Y182), but the band was very light.

Anti-p38 (phospho T180 + Y182) antibody from Abcam

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Anti-p38 (phospho T180 + Y182) antibody from Abcam

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