Fig 1: Increased proliferation of cells appears to be mediated by enhanced SHH signalling. (A-D) RT-PCR analysis of SHH effector genes and target genes. (A, B) Gli1 and Gli2 were upregulated expressed in the mRNA level at P10 in the CTX-treated group. (C, D) The expression of SHH target genes N-Myc and Ccnd1 was upregulated significantly at P10, P < 0.001. (E) Immunoblotting of proteins related to SHH signalling at P10 showed that SHH signalling is enhanced to mediate the restoration of the CTX-induced deficit in the developing cerebella