Fig 2: Retained expression of FAM107A in non-tumor controls (K samples; panel a) and lack of FAM107A expression in laryngeal cancer samples: cell lines (UT samples; panel b and c) and primary tumors (MK samples; panel c and d). GAPDH expression was used as the internal control. NTC – no template control. Size marker PerfectTM 100–1000 bp DNA Ladder (Eurx).

Fig 3: Mean methylation of FAM107A promoter region in analyzed cell lines, primary tumors and non-cancer controls. The flat black line indicates the cut off value of 41%, above which samples are assigned as hypermethylated.

Fig 4: The structure of FAM107A gene. Three marked CG dinucleotides within the promoter region of the gene were amplified for DNA methylation (intron - exon not to scale). TSS –Transcription Start Site.

Fig 5: The relative expression of FAM107A indicated by two tags – (a) 209074_s_at and (b) 207547_s_at. Significantly downregulated expression of FAM107A in laryngeal primary tumors and cell lines is shown (p < 0.05; U Mann Whitney test; GraphPad Prism 7 demo version).