Fig 1: VFC CAR T cells induce greater cytolysis of GD2+ GSC spheroids when compared with RV CAR T cells. (A) Representative confocal images of immunostained GSC spheroids after 48 h of co-culture with 1:1 RV CAR T or VFC CAR T cells compared with no treatment GSC spheroids alone. Spheroids were stained for DAPI, GD2, CD3 and CC3. Scale bar, 200 mm. Spheroid imaging was used to quantify GSC cell density within each spheroid, (C) an average number of T-cell infiltrates per spheroid, (D) difference in the percentage of GD2+ GSCs per spheroid across treatments and (E) percentage of CC3+ GSCs per spheroid at 48-h endpoint. N = 20. Error bars represent mean ± standard deviation. ****P < 0.0001 determined by one-way ANOVA with post-hoc Tukey test.