PE anti-HA.11 Epitope Tag 901518 from BioLegend

Product Specs
ItemPE anti-HA.11 Epitope Tag
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number901518
Quantity100 µg
ApplicationsFlow Cytometry (FC), Intracellular Staining for Flow Cytometry (ICFC)
Concentration0.20 mg/mL
Conjugate/TagPE
IsotypeIgG1, Kappa
Clone16B12
HostMouse
ImmunogenMonoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL
ClonalityMonoclonal
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
TargetHA.11

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Stringent and complex sequence constraints of an IGHV1-69 broadly neutralizing antibody to influenza HA stembioRxivJuly 13, 2023Qi Wen Teo, Yiquan Wang, Huibin Lv, Timothy J.C. Tan, Ruipeng Lei, Kevin J. Mao, Nicholas C. Wuresuspended in 1 mL of 1× PBS. For expression sort, PE anti-HA.11 (epitope 16B12, BioLegend, Cat. No. 901517) that was buffer-exchanged into 1× PBS was added to the cells at a final concentration of 1 µg/mL. For

(2) Computational design of dynamic receptor—peptide signaling complexes applied to chemotaxisNature CommunicationsMay 19, 2023Robert E. Jefferson, Aurélien Oggier, Andreas Füglistaler, Nicolas Camviel, Mahdi Hijazi, Ana Rico Villarreal, Caroline Arber, Patrick Barthwith a PE selection kit (EasySep, ref: 17684) and an anti-HA PE-conjugated antibody (Biolegend, ref: 901518, clone: 16B12) at a 1:50 dilution to enrich transduced T cells. Enrichment of surface expression in

(3) Itch receptor MRGPRX4 interacts with the receptor activity-modifying proteins (RAMPs)Journal of Biological ChemistryMay 1, 2023Ilana B. Kotliar, Emilie Ceraudo, Kevin Kemelmakher-Liben, Deena A. Oren, Emily Lorenzen, Tea Dodig-Crnković, Mizuho Horioka-Duplix, Thomas Huber, Jochen M. Schwenk, Thomas P. Sakmar(926-32210) were from LI-COR Biosciences. PE-conjugated rat anti-FLAG (637309), PE-conjugated mouse anti-HA (901517), and mouse anti-HA (16B12) were from BioLegend. Sheep anti-RAMP1, anti-RAMP2, and anti-RAMP3 (AF6428,

(4) Prevalence and mechanisms of evolutionary contingency in human influenza H3N2 neuraminidaseNature communicationsOctober 28, 2022Lei R, Tan TJC, Hernandez Garcia A, Wang Y, Diefenbacher M, Teo C, Gopan G, Tavakoli Dargani Z, Teo QW, Graham CS, et al.resuspended in 1 mL FACS buffer. Cells were incubated with 1 μg mL−1 PE anti-HA.11 (BioLegend, catalog #: 901518) at 4 °C with rocking for 1 h. Subsequently, cells were pelleted via centrifugation at 300 \documentclass[12pt]{minimal}

(5) Sequence signatures of two IGHV3-53/3-66 public clonotypes to SARS-CoV-2 receptor binding domainbioRxivJuly 12, 2022Timothy J.C. Tan et al.ml of 1x PBS. Then, for expression assay, 1 μg of PE anti-HA.11 (epitope 16B12, BioLegend, Cat. No. 901517) buffer-exchanged into 1x PBS was added to the cells. A negative control was set up with nothing added

(6) CRISPR/Cas9 unveils the dynamics of the endogenous µ‐opioid receptors on neuronal cells under continuous opioid stimulationPharmacology Research & PerspectivesApril 1, 2022Satoshi Shimizu, Atsuko ShirakiAbcam). For flow cytometry analysis, we used phycoerythrin (PE) anti‐HA.11 epitope tag antibody (1:100, 901518; BioLegend) PE anti‐mouse IgG1, and κ Isotype Ctrl Antibody (1:100, 400111; BioLegend). 2.6 Flow cytometry analysis

(7) Pharmacological chaperones for the oxytocin receptor increase oxytocin responsiveness in myometrial cellsThe Journal of biological chemistryMarch 1, 2022Malik M, Fang Y, Wakle-Prabagaran M, Roh M, Prifti K, Frolova AI, Imoukhuede PI, England SK.19). An empirically determined saturating concentration of phycoerythrin-conjugated anti-HA antibody (901518, Biolegend) was used for each cell type (16 ng/μl for transfected HEK293T cells, 10 ng/μl for hTERT-HM

(8) Sequence signatures of two public antibody clonotypes that bind SARS-CoV-2 receptor binding domainNature CommunicationsDecember 1, 2021Timothy J. C. Tan et al.resuspended in 1 ml of 1× PBS. Then, for expression assay, PE anti-HA.11 (epitope 16B12, BioLegend, Cat. No. 901517) that was buffer-exchanged into 1× PBS was added to the cells at a final concentration of 1 µg/ml. A

(9) Naturally Occurring Genetic Variants in the Oxytocin Receptor Alter Receptor Signaling ProfilesACS Pharmacology & Translational ScienceOctober 8, 2021Manasi Malik et al.determined saturating concentration (8–16 μg/mL) of phycoerythrin (PE)-conjugated anti-HA antibody (901518, Biolegend, San Diego, CA) in staining buffer (0.5% BSA and 0.1% sodium azide in Ca2+/Mg2+-free PBS)

(10) Optimization of AsCas12a for combinatorial genetic screens in human cellsNature BiotechnologyJanuary 1, 2021Peter C. DeWeirdt et al.temperature, washed with chilled PBS, permeabilized (ab185917) and stained with PE anti-HA (Biolegend #901518) for 30 minutes at room temperature, washed with chilled PBS twice to remove residual antibody, and

(11) Genotypic Regulation of Type I Interferon Induction Pathways by Frameshift (F) Proteins of Hepatitis C VirusJournal of virologyJuly 16, 2020Lai YT, Liou YM, Hsin F, Liu HM.cultured cells were stained with phycoerythrin (PE)-conjugated anti-HA.11 epitope tag (catalog no. 901517; BioLegend) at 4°C in the dark for 30 min. BD FACSCalibur was used for analyzing the ﬂuorescence. PE-positive

(12) HMCES Functions in the Alternative End-Joining Pathway of the DNA DSB Repair during Class Switch Recombination in B CellsMolecular cellJanuary 16, 2020Shukla V, Halabelian L, Balagere S, Samaniego-Castruita D, Feldman DE, Arrowsmith CH, Rao A, Aravind L.AB_2794375" anti-mouse IgM APC conjugated,Biolegend,"#406509, RRID:" ,,AB_315059 anti-HA PE conjugated,Biolegend,"#901518, RRID:AB_2629623" anti-IgG1 PE conjugated,Biolegend,"#406608, RRID: AB_10551618" ,, "Chemicals, Peptides,

(13) Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migrationNature communicationsMay 23, 2014Majeed SR, Vasudevan L, Chen CY, Luo Y, Torres JA, Evans TM, Sharkey A, Foraker AB, Wong NM, Esk C, et al.

(14) Splice variants of SmgGDS control small GTPase prenylation and membrane localizationThe Journal of biological chemistryNovember 12, 2010Berg TJ, Gastonguay AJ, Lorimer EL, Kuhnmuench JR, Li R, Fields AP, Williams CL.

(15) Gly-46 and His-50 of yeast maltose transporter Mal21p are essential for its resistance against glucose-induced degradationThe Journal of biological chemistryJune 5, 2009Hatanaka H, Omura F, Kodama Y, Ashikari T.

(16) Critical role of the pleckstrin homology domain in Dbs signaling and growth regulationThe Journal of biological chemistryJune 6, 2003Fuentes EJ, Karnoub AE, Booden MA, Der CJ, Campbell SL.

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PE anti-HA.11 Epitope Tag from BioLegend

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PE anti-HA.11 Epitope Tag from BioLegend

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