Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker ab9023 from Abcam

Product Specs
ItemAnti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab9023
Antibody TypePrimary
CloneM20
IsotypeIgG1
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Western blot
Quantity100 UL
ClonalityMonoclonal
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameKRT8
ValidationKnockOut Validated
ReactivityHuman
TargetCytokeratin 8

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Single-cell transcriptome analysis indicates fatty acid metabolism-mediated metastasis and immunosuppression in male breast cancerNature CommunicationsSeptember 11, 2023Handong Sun, Lishen Zhang, Zhonglin Wang, Danling Gu, Mengyan Zhu, Yun Cai, Lu Li, Jiaqi Tang, Bin Huang, Bakwatanisa Bosco, et al.temperature. The samples were incubated with a primary antibody (CD3, #17617-1-AP, Proteintech, 1:500; KRT8, #ab9023, Abcam, 1:200) overnight at 4 °C (Supplementary Data 12). The following day, after washing with PBS,

(2) YAP is required for prostate development, regeneration, and prostate stem cell functionCell Death DiscoverySeptember 9, 2023Hui Xie, Linpei Guo, Qianwang Ma, Wenyi Zhang, Zhao Yang, Zhun Wang, Shuanghe Peng, Keruo Wang, Simeng Wen, Zhiqun Shang, et al.(Cell Signaling Technology 12202 S), CK5 (Abcam ab52635), CK14 (Proteintech Group 60320-1-Ig) and CK8 (Abcam ab9023 or ab53280). Then second antibody was added and incubated for 1 h. Development was achieved through

(3) Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoidsSTAR protocolsSeptember 16, 2022Ryosaka M, Mae SI, Osafune K.AB_2810957 Goat anti-BRACHYURY (1:500),R&D,Cat#AF2085;RRID: AB_2200235 Mouse anti-CK8 (1:500),Abcam,Cat#ab9023;RRID: AB_306948 Mouse anti-E-CADHERIN (1:500),BD,Cat#610181;RRID: AB_397580 Goat anti-E-CADHERIN (1:200),R&D,Cat#AF648;RRID:

(4) Reducing tumor invasiveness by ramucirumab and TGF‐β receptor kinase inhibitor in a diffuse‐type gastric cancer patient‐derived cell modelCancer MedicineOctober 1, 2021Song‐Yi Lee, Seonggyu Byeon, Jihoon Ko, Sujin Hyung, In‐Kyoung Lee, Noo Li Jeon, Jung Yong Hong, Seung Tae Kim, Se Hoon Park, Jeeyun Lee(Ser 465/467)/Smad3 (Ser 423/425) (#8828, 1:1000; CST), β‐catenin (#8480, 1:1000; CST), cytokeratin (ab9023, 1:2000; Abcam), and β‐actin (AC‐15, 1:5000; Sigma) An ECL system was used for protein detection (Invitrogen). 2.4

(5) A multi-scale integrated analysis identifies KRT8 as a pan-cancer early biomarkerPac Symp BiocomputMarch 10, 2021Madeleine K. D. Scott, Michael G. Limaye, Pauline Schaffert, Maneesha Limaye, Michael G Ozawa, Pauline Schaffert, Albert C. Koong, Albert C Koong, Purvesh Khatri(ab109452, Burlingame, CA). Mouse monocolonal Anti-Cytokeratin 8 antibody was also obtained from Abcam (ab9023, Burlingame, CA). The intensity of KRT8 immunostaining was graded from 1–4 as determined by an independent

(6) Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury ModelingCell reportsDecember 15, 2020Uchimura K, Wu H, Yoshimura Y, Humphreys BD.,R&D Systems,Cat#bba7; RRID: AB_356960 anti-NPHS1,R&D Systems,Cat#AF4269; RRID: AB_2154851 anti-CK8,Abcam,Cat#ab9023; RRID: AB_306948 anti-Na/K/ATPase,Abcam,Cat#ab76020; RRID: AB_1310695 anti-HAVCR1,R&D Systems,Cat#AF1750;

(7) Inorganic arsenic promotes luminal to basal transition and metastasis of breast cancer.The FASEB JournalDecember 1, 2020Jeanne M. Danes, Andre L. P. de Abreu, Romica Kerketta, Yunping Huang, Flavio R. Palma, Benjamin N. Gantner, Angela J. Mathison, Raul A. Urrutia, Marcelo G. Bonini3 min, incubated with primary antibody (dilution 1:75), CK5 (Abcam, catalog# 17130) and CK8 (Abcam ab9023) overnight at 4°C. Mammospheres were then washed 3x with 1X TBST and incubated with secondary antibody

(8) Estrogen attenuates TGF-β1-induced EMT in intrauterine adhesion by activating Wnt/β-catenin signaling pathwayBrazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicasJanuary 1, 2020Cao J, Liu D, Zhao S, Yuan L, Huang Y, Ma J, Yang Z, Shi B, Wang L, Wei J.Number,Company,Specificity,Antibody dilution,Antibody dilution ,,,,WB,IF GAPDH,ab128915,Abcam,Rabbit monoclonal,1:5000,1:200 CK8,ab9023,Abcam,Mouse monoclonal,1:1000,1:200 CK7,ab181598,Abcam,Rabbit monoclonal,1:1000,1:200 EPCAM,ab71916,Abcam,Rabbit

(9) Perfusion culture maintained with an air-liquid interface to stimulate epithelial cell organization in renal organoids in vitroBMC Biomedical EngineeringDecember 1, 2019Sachiko Sekiya, Tetsutaro Kikuchi, Tatsuya ShimizuAldrich, St. Louis, MO, USA) for 1 h. They were then incubated with Laminin (L9393, Sigma-Aldrich), CK8 (ab9023, Abcam, Cambridge UK), and podocalyxin (AF1658 R&D Systems, Minneapolis, MN, USA) antibodies at 4 °C.

(10) The vacuolated morphology of chordoma cells is dependent on cytokeratin intermediate filamentsJournal of cellular physiologyApril 1, 2019Resutek L, Hsieh AH.(Thermo Fisher Scientific, Cat# MA5-16409 , AB_2537928), or mouse IgG1 anti-cytokeratin-8 [M20] (Abcam, Cat# ab9023 RRID:AB_306948) antibodies. A biotinylated (anti-rabbit IgG) secondary antibody (Vector Laboratories,

(11) Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversityNature communicationsMay 23, 2018Nguyen QH, Pervolarakis N, Blake K, Ma D, Davis RT, James N, Phung AT, Willey E, Kumar R, Jabart E, et al.scWest chip was washed 2 × 10 min in 1× Wash Buffer, then probed for mouse anti-cytokeratin 8 (Abcam ab9023) at 200 µg/mL and rabbit anti-β-tubulin (Abcam ab6046) at 100 µg/mL for 2 h at room temperature. Primary

(12) Annexin A1 contributes to pancreatic cancer cell phenotype, behaviour and metastatic potential independently of Formyl Peptide Receptor pathwayScientific reportsJuly 14, 2016Belvedere R, Bizzarro V, Forte G, Dal Piaz F, Parente L, Petrella A.

(13) Potentially positive ageing-related variations of medial smooth muscle cells in the saphenous veins used as aortocoronary bypass graftsFolia Histochemica et CytobiologicaJanuary 13, 2016Bartlomiej Perek, Agnieszka Malinska, Jerzy Gasowski, Danuta Ostalska-Nowicka, Anna Perek, Marek Jemielity, Maciej Zabel, Michal Nowickiantibodies: anti-a-SM actin (M0851, Dako), anti-SM-MHC (MAB13431, Dako), anti-CALP (M3556, Dako), anti-CK-8 (ab9023; Abcam, Cambridge, UK), anti-MMP-2 (MAB13431, Millipore, Darmstadt, Ger- www.fhc.viamedica.pl 93 Venous

(14) ZnT4 (SLC30A4)-null (“lethal milk”) mice have defects in mammary gland secretion and hallmarks of precocious involution during lactationAmerican Journal of Physiology-Regulatory, Integrative and Comparative PhysiologyJanuary 1, 2016Nicholas H. McCormick, Sooyeon Lee, Stephen R. Hennigar, Shannon L. Kelleherprimary antibodies for 1 h: ZnT4, [1:1,000, (19)], ZnT2 [1 ␮g/ml, (17)], cytokeratin 8 (CK8, 1:1,000, no. ab9023; Abcam, Cambridge, MA) phospho-STAT3 (1: 1,000, no. 9131; Cell Signaling Technology, Danvers, MA), STAT3 (1:1,000;

(15) Inflammation decreases keratin level in ulcerative colitis; inadequate restoration associates with increased risk of colitis-associated cancerBMJ Open GastroenterologyDecember 31, 2015Bernard M Corfe, Debabrata Majumdar, Arash Assadsangabi, Alexandra M R Marsh, Simon S Cross, Joanne B Connolly, Caroline A Evans, Alan J Loboand immunoblotting were as previously described.4 Primary antibodies were mouse monoclonal: anti-K8 (ab9023); K18 (ab668), K19 (ab7754); K8 phospho-specific rabbit antiphospho S73 antibody (ab32579) and phospho

(16) Expression analysis of α-smooth muscle actin and tenascin-C in the periodontal ligament under orthodontic loading or in vitro cultureInternational Journal of Oral ScienceDecember 18, 2015Hui Xu, Ding Bai, L-Bruno Ruest, Jian Q Feng, Yong-Wen Guo, Ye Tian, Yan Jing, Yao He, Xiang-Long Han

(17) Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in cultureNature communicationsNovember 13, 2015Fridriksdottir AJ, Kim J, Villadsen R, Klitgaard MC, Hopkinson BM, Petersen OW, Rønnov-Jessen L.use",—,— ERα,1D5,"Dako, M7047",1:100,1:25,— GATA3,HG3–31,"Santa Cruz, sc-268",—,1:25,— Keratin 8,M20,"Abcam, ab9023",1:50,1:50,— Keratin 8,TS1,"Novocastra, NCL-CK8-TS1",1:50,—,— Keratin 20*,Ks20.8,"Dako, M7019",1:25,1:10,— Keratin

(18) Reduced keratin expression in colorectal neoplasia and associated fields is reversible by diet and resectionBMJ open gastroenterologyJanuary 1, 2015Evans CA, Rosser R, Waby JS, Noirel J, Lai D, Wright PC, Williams EA, Riley SA, Bury JP, Corfe BM.

(19) Chlamydia infection depends on a functional MDM2-p53 axisNature CommunicationsDecember 1, 2014Erik González, Marion Rother, Markus C. Kerr, Munir A. Al-Zeer, Mohammad Abu-Lubad, Mirjana Kessler, Volker Brinkmann, Alexander Loewer, Thomas F. MeyerSanta Cruz Biotechnology Inc.; anti-Chlamydia trachomatis MOMP (ab20881, 1:2,000), anti-cytokeratin-8 (ab9023, 1:8,000) and anti-vimentin (ab16700, 1:2,000) antibodies were purchased from Abcam, mouse monoclonal

(20) Transient induction of ING4 by Myc drives prostate epithelial cell differentiation and its disruption drives prostate tumorigenesisCancer researchJune 15, 2014Berger PL, Frank SB, Schulz VV, Nollet EA, Edick MJ, Holly B, Chang TT, Hostetter G, Kim S, Miranti CK.

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Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker from Abcam

Abcam

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Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker from Abcam

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