Anti-HEC1/HEC antibody [9G3] ab3613 from Abcam

Product Specs
ItemAnti-HEC1/HEC antibody [9G3]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab3613
Antibody TypePrimary
Quantity100 UL
ClonalityMonoclonal
HostMouse, Nude mouse
IsotypeIgG2a
Clone9G3
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameNDC80
ReactivityHuman, Pig
TargetHEC1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Dynamic phosphorylation of CENP-N by CDK1 guides accurate chromosome segregation in mitosisJournal of molecular cell biologyNovember 27, 2023Liu R, Dou Z, Tian T, Gao X, Chen L, Yuan X, Wang C, Hao J, Gui P, Mullen M, et al.Cell Signaling Technology, 49426), anti-Nuf2 (Abcam, ab122962), ACA (Immunovision, HCT-0100), anti-HEC1 (Abcam, ab3613), anti-CENP-L (kindly gifted by Dr Iain Cheeseman), and anti-CENP-N (kindly gifted by Dr Iain Cheeseman)

(2) A bifunctional kinase–phosphatase module balances mitotic checkpoint strength and kinetochore–microtubule attachment stabilityThe EMBO JournalOctober 16, 2023Andrea Corno, Marilia H Cordeiro, Lindsey A Allan, Qian‐Wei Lim, Elena Harrington, Richard J Smith, Adrian T Saurin(ab200061 from Abcam, 1:1,000), mouse anti‐MAD1 (MABE867 from Millipore, 1:1,000), mouse anti‐HEC1 (ab3613 from Abcam, 1:2,000) and mouse anti‐FLAG(M2) (F3165‐.2MG from Sigma, 1:2,000). The rabbit anti‐pMELT‐KNL1

(3) Oocytes can repair DNA damage during meiosis via a microtubule-dependent recruitment of CIP2A-MDC1-TOPBP1 complex from spindle pole to chromosomesNucleic acids researchJune 9, 2023Leem J, Kim JS, Oh JS.in an IBMX-free medium for 4 h and then microinjected with CENP-A (2048S, Cell Signaling) or HEC1 (ab3613, Abcam) antibodies at a final concentration of 100 ng/μl. Control oocytes were injected with normal

(4) Coordination between the Ndc80 complex and dynein is essential for microtubule plus-end capture by kinetochores during early mitosisJournal of Biological ChemistryJune 1, 2023Mohammed Abdullahel Amin, Manas Chakraborty, Destiny Ariel Wallace, Dileep VarmaCLIP-170 (200 nM). Antibodies The primary monoclonal mouse antibodies used were anti-Hec1 9G3 (Abcam, ab3613) for immunoflourescence (IF) at 1:400 and for WB at 1:1000; anti-dynein intermediate chain (DIC) clone

(5) PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle sizeThe Journal of cell biologyMay 1, 2023Sobajima T, Kowalczyk KM, Skylakakis S, Hayward D, Fulcher LJ, Neary C, Batley C, Kurlekar S, Roberts E, Gruneberg U, et al.sc-515550; for immunoprecipitation only: rabbit pAb, ProteinTech, 18932-1-AP and mouse mAb, Abcam, ab3613; for immunofluorescence: mouse mAb [clone 9G3], ab3613, Abcam), MPS1 (rabbit pAb, 10381-1-AP; ProteinTech),

(6) Semi-automated 3D fluorescence speckle analyzer (3D-Speckler) for microscope calibration and nanoscale measurementJournal of Cell BiologyApril 3, 2023Jonathan Loi, Xiaofei Qu, Aussie SuzukiDepartment of Biochemistry, Stanford University, Stanford, CA, USA; Wan et al., 2009), mouse anti-Hec1 (9G3, ab3613; Abcam), and mouse anti-tubulin (#3873; DM1A, mAb). Minimum cross-react Donkey anti-rabbit and mouse

(7) Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblyNature communicationsNovember 27, 2022Matković J, Ghosh S, Ćosić M, Eibes S, Barišić M, Pavin N, Tolić IM.Abcam, Cambridge, UK), diluted 1:500, Rabbit anti-Aurora B (ab239837, Abcam), diluted 1:100, anti-Hec1 (ab3613, Abcam), diluted 1:100, anti-Spindly (A301-354A, Biomolecules), diluted 1:100, anti-phospho-CENP-A (Ser7)

(8) Micronuclei from misaligned chromosomes that satisfy the spindle assembly checkpoint in cancer cellsCurrent BiologyOctober 10, 2022Ana Margarida Gomes, Bernardo Orr, Marco Novais-Cruz, Filipe De Sousa, Joana MacÃ¡rio-Monteiro, Carolina Lemos, Cristina FerrÃ¡s, Helder MaiatoRRID:AB_10693556 mouse anti-Aim1,BD Biosciences,Cat#611083; RRID:AB_398396 mouse anti-Hec1 (9GA),Abcam,Ab3613; RRID:AB_303949 mouse anti-Dsn1,Gift from A. Musacchio,N/A mouse anti-ATRX,Santa Cruz Biotechnology,sc-55584;

(9) Phosphorylation of CENP-R by Aurora B regulates kinetochore-microtubule attachment for accurate chromosome segregationJournal of molecular cell biologySeptember 27, 2022Sedzro DM, Yuan X, Mullen M, Ejaz U, Yang T, Liu X, Song X, Tang YC, Pan W, Zou P, et al.Technology), mouse anti-FLAG (F3165, Sigma), anti-Cyclin B1 (12231, Cell Signaling Technology), anti-Hec1 (AB3613, Abcam), anti-Aurora B (611082, BD), anti-CENP-U (HPA022048, Atlas), and human anti-centromere auto-antibody

(10) Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubulesNature CommunicationsAugust 10, 2022Cédric Castrogiovanni, Alessio V. Inchingolo, Jonathan U. Harrison, Damian Dudka, Onur Sen, Nigel J. Burroughs, Andrew D. McAinsh, Patrick Meraldianti-α-tubulin (1:30054), rabbit anti-α-tubulin (1:1000; Abcam; ab18251), mouse anti-Hec1 (1:700; Abcam; ab3613), rabbit anti-Mad2 (1:1000; Bethyl; A300-301A), and mouse anti-EB1 (1:200; BD biosciences; 610535).

(11) The Astrin-SKAP complex reduces friction at the kinetochore-microtubule interfaceCurrent BiologyJune 1, 2022Miquel Rosas-Salvans, Renaldo Sutanto, Pooja Suresh, Sophie DumontJ. DeLuca25), CREST (1:100, human, Antibodies Incorporated, 15–234-0001), Hec1 (1:200, mouse, Abcam, ab3613), tubulin (1:2000, rat, Bio-rad, MCA77G). There washes in IF buffer (10 min each) were done before incubation

(12) Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammalsCell reportsApril 5, 2022Almeida AC, Soares-de-Oliveira J, Drpic D, Cheeseman LP, Damas J, Lewin HA, Larkin DM, Aguiar P, Pereira AJ, Maiato H.polyclonal anti-TPX2,Proteintech,"Cat# 11741-1-AP, RRID:AB_2208895" Mouse monoclonal anti-HEC1,Abcam,"Cat# ab3613, RRID:AB_303949" Rabbit polyclonal anti-Mis12,"Gift from C. Sunkel (Feijão et al., 2013)",N/A Rabbit

(13) Non-centrosomal microtubules at kinetochores promote rapid chromosome biorientation during mitosis in human cellsCurrent BiologyMarch 1, 2022Fioranna Renda, Christopher Miles, Irina Tikhonenko, Rebecca Fisher, Lina Carlini, Tarun M. Kapoor, Alex Mogilner, Alexey Khodjakovwere obtained as previously described26. KTs and MTs were visualized with monoclonal 9G3/Hec1 (Abcam ab3613) at 1:200 and DM1α antibody (Sigma T9026) antibodies followed by isotype-specific secondary antibodies

(14) Recruitment of two Ndc80 complexes via the CENP-T pathway is sufficient for kinetochore functionsNature communicationsFebruary 14, 2022Takenoshita Y, Hara M, Fukagawa T.rabbit anti-chicken Aurora B34 (1:5000), mouse anti-H3T3ph34 (1:10000), mouse anti-human Ndc80 (Abcam, ab3613) (1:1000), mouse anti-FLAG-M2 (Sigma, F1804) (1:1000), mouse anti-α-tubulin (Sigma T9026) (1:5000),

(15) Subcellular Euclidean distance measurements with multicolor fluorescence localization imaging in cultured cellsSTAR ProtocolsDecember 1, 2021Tsvetelina E. Germanova, Emanuele Roscioli, Jonathan U. Harrison, Andrew D. McAinsh, Nigel J. Burroughsmonoclonal anti-CenpA (1:300),Enzo,Cat#ADI-KAM-CC006-E "Mouse monoclonal anti-Hec1 (9G3, 1:1000)",Abcam,Cat#ab3613 Mouse monoclonal anti-Bub1 (1:200),Abcam,Cat#ab54893 Mouse monoclonal anti-Rod (1:50),Abcam,Cat#ab56745 Mouse

(16) Chromosome oscillation promotes Aurora A-dependent Hec1 phosphorylation and mitotic fidelityThe Journal of cell biologyJuly 5, 2021Iemura K, Natsume T, Maehara K, Kanemaki MT, Tanaka K.methods Plasmids and antibodies Monoclonal mouse antibodies were used as follows: anti-Hec1 9G3 (Abcam; ab3613) for Western blotting (WB) 1:2,000, for IF 1:1,000; anti–α-tubulin B-5-1-2 (Merck; T5168) WB 1:5,000,

(17) VTT-006, an anti-mitotic compound, binds to the Ndc80 complex and suppresses cancer cell growth in vitroOncoscienceJanuary 1, 2021Laine LJ, Mäki-Jouppila JHE, Kutvonen E, Tiikkainen P, Nyholm TKM, Tien JF, Umbreit NT, Härmä V, Kallio L, Davis TN, et al.phospho-Thr232 (Rockland 600-401-677), Bub1 (Upstate, 05-899), BuBR1 (Abcam, ab4637), Hec1 (Abcam, ab3613), pericentrin (Abcam, ab4448), tubulin (Abcam, ab7291) or human autoimmune serum (Crest, Antibodies

(18) H3K9me3 maintenance on a Human Artificial Chromosome is required for segregation but not centromere epigenetic memoryJournal of Cell ScienceJuly 15, 2020Nuno M. C. Martins et al.anti-Aurora B, 1:1000 dilution (ab2254, Abcam, Cambridge, MA); rabbit anti-Hec1, 1:10,000 dilution (Ab3613, Abcam), rabbit anti-Gallus gallus CENP-A, 1:500 dilution (Régnier et al., 2003); human, ACA 1:100 dilution

(19) Effects of malleable kinetochore morphology on measurements of intrakinetochore tensionOpen biologyJuly 1, 2020Renda F, Magidson V, Tikhonenko I, Fisher R, Miles C, Mogilner A, Khodjakov A.Thermo Fisher Scientific). Outer kinetochore domain was stained with a monoclonal 9G3/Hec1 antibody (Abcam ab3613) at 1 : 1000 or a rabbit polyclonal antibody against a C-terminal of CenpF (NB500–101; Novus Biological

(20) Mitotic checkpoint protein Mad1 is required for early Nup153 recruitment to chromatin and nuclear envelope integrityJournal of Cell ScienceMay 21, 2020Ikram Mossaid, Guillaume Chatel, Valérie Martinelli, Marcela Vaz, Birthe Fahrenkrog(Abcam, Cambridge, UK; ab40567; IF 1:60); mouse anti-FLAG (Sigma-Aldrich; F3165; WB 1:2000); mouse anti-Hec1 (Abcam; ab3613; IF 1:200); mouse mAb414 (Covance, Mechelen, Belgium; IF 1:5000); mouse anti-importin β (BD Biosciences,

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Anti-HEC1/HEC antibody [9G3] from Abcam

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Anti-HEC1/HEC antibody [9G3] from Abcam

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