Fig 1: (A) Structure of acetyl-lysine 3,5-dinitrobenzyl ester (AcK-DBE) and thioacetyl-lysine 3,5-dinitrobenzyl ester (ThioAcK-DBE). (B) Western Blots of single or dual ncAAs (AcK, ThioAcK) residues into H4 variants (H4K16_ThioAck/H4K91_AcK) with site-specific anti-acetyl histone antibodies (Abcam, ab109463, ab4627). 10 µL of the corresponding PURE reaction solution was loaded into each lane.
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