Anti-Glucose Transporter GLUT4 antibody ab654 from Abcam

Product Specs
ItemAnti-Glucose Transporter GLUT4 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab654
Antibody TypePrimary
Quantity250 UL
HostRabbit
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
IsotypeIgG
ClonalityPolyclonal
Conjugate/TagUnconjugated
Gene NameSLC2A4
ReactivityHuman
TargetGlucose Transporter GLUT4

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Glucose Restriction Enhances Oxidative Fibre Formation: A multi-omic signal network involving AMPK and CaMK2iScienceNovember 29, 2023Kaiyi Zhang, Ning Xie, Huaqiong Ye, Jiakun Miao, Boce Xia, Yu Yang, Huanqi Peng, Shuang Xu, Tianwen Wu, Cong Tao, et al.of denatured total protein were loaded into each line. Primary antibodies recognizing GLUT4 (Abcam ab654), GLUT1 (Abcam ab115730), COX IV (CST #11967), SCD-1(CST #2794), phospho-AMPK (CST #2535), total AMPK

(2) Changes to insulin sensitivity in glucose clearance systems and redox following dietary supplementation with a novel cysteine-rich protein: A pilot randomized controlled trial in humans with type-2 diabetesRedox BiologyNovember 1, 2023W.M. Peeters, M. Gram, G.J. Dias, M.C.M. Vissers, M.B. Hampton, N. Dickerhof, A.E. Bekhit, M.J. Black, J. Oxbøll, S. Bayer, et al.visualization of insulin signalling: 1:250 anti-GLUT4 rabbit polyclonal primary antibody (Abcam, catalogue # ab654) [34], 1:500 mouse polyclonal anti-dystrophin (Sigma-Aldrich, catalogue #D8168), 1:500 rabbit polyclonal

(3) GABA Prevents Age-Related Sarcopenic Obesity in Mice with High-Fat-Diet-Induced ObesityCellsAugust 25, 2023Heegu Jin, Hyun-Ji Oh, Boo-Yong Lee(ab202344), UCP1, NRF1 (ab175932), TFAM (ab131607), UCP3 (ab10985), p-PI3K (Tyr 607, ab182651), GLUT4 (ab654), Atrogin-1 (ab168372), MuRF1 (ab172479), myostatin (ab203076), and Myf5 (ab125301) were purchased from

(4) GDF15 promotes weight loss by enhancing energy expenditure in muscleNatureJuly 6, 2023Dongdong Wang, Logan K. Townsend, Geneviève J. DesOrmeaux, Sara M. Frangos, Battsetseg Batchuluun, Lauralyne Dumont, Rune Ehrenreich Kuhre, Elham Ahmadi, Sumei Hu, Irena A. Rebalka, et al.(1:500, OXPHOS cocktail, Abcam, Ab110413), COXIV (1:30,000, Invitrogen, A21347), GLUT4 (1:2,500, Abcam, Ab654), calnexin (1:2,000, Sigma-Aldrich, C4731) and SERCA2 (1:1,000, Abcam ab2861). Respiration in permeabilized

(5) Brain insulin signaling suppresses lipolysis in the absence of peripheral insulin receptors and requires the MAPK pathwayMolecular MetabolismJuly 1, 2023Matthäus Metz, James O'Hare, Bob Cheng, Michelle Puchowicz, Christoph Buettner, Thomas Scherer4 °C. Primary antibodies against IRβ (sc-57342, Santa Cruz Biotechnology, Santa Cruz, CA), Glut 4 (ab654, Abcam, Cambridge UK), Akt (#9272), phos Akt (#4060), Erk (#9102), phos Erk (#9101), Hsl (#4107), phos

(6) [(18)F]FDG Uptake and Expression of Immunohistochemical Markers Related to Glycolysis, Hypoxia, and Proliferation in Indeterminate Thyroid NodulesMolecular imaging and biologyJune 1, 2023de Koster EJ, van Engen-van Grunsven ACH, Bussink J, Frielink C, de Geus-Oei LF, Kusters B, Peters H, Oyen WJG, Vriens D, EFFECTS trial study group.dilution 1:500), GLUT3 (RB-9096-P, Immunologic, WellMed BV, Duiven, the Netherlands; 1:300), GLUT4 (ab654, Abcam, Cambridge, UK: 1:1000), HK1 (MA5-15,680, Invitrogen™, Thermo Fisher Scientific; 1:7500), HK2

(7) Naringenin and β-carotene convert human white adipocytes to a beige phenotype and elevate hormone- stimulated lipolysisFront Endocrinol (Lausanne)April 17, 2023Ann A. Coulter, Frank L. Greenway, Dachuan Zhang, Sujoy Ghosh, Cathryn R. Coulter, Sarah L. James, Yanlin He, Luke A. Cusimano, Candida J. RebelloLouis, Mo) unless otherwise indicated. Primary antibodies used were UCP1 (#MAB6158, R&D Systems), GLUT4 (Ab654 Abcam), PGC-1α (ST1202, Sigma) and β-Actin (A5316, Sigma), or monoclonals from Santa Cruz against ATGL

(8) Desmin Knock-Out Cardiomyopathy: A Heart on the Verge of Metabolic CrisisInternational Journal of Molecular SciencesOctober 10, 2022Barbara Elsnicova, Daniela Hornikova, Veronika Tibenska, David Kolar, Tereza Tlapakova, Benjamin Schmid, Markus Mallek, Britta Eggers, Ursula SchlÃ¶tzer-Schrehardt, Viktoriya Peeva, et al.ab78259, 1:50, respectively), rabbit polyclonal anti-Glut1 and anti-Glut4 (Abcam, ab115730, 1:50, and ab654, 1:50, respectively), and rabbit polyclonal anti-CKB and anti-CKM (Abcam, ab92452, 1:50, and ab189438,

(9) The conserved Mediator subunit cyclin C (CCNC) is required for brown adipocyte development and lipid accumulationMolecular metabolismOctober 1, 2022Song Z, Xiaoli AM, Li Y, Siqin G, Wu T, Strich R, Pessin JE, Yang F.1:250), Anti-FAS (3180, 1:1000) and Anti-ACC1 (3676, 1:1000) from Cell Signaling Technology; Anti-GLUT4 (ab654, 1:500) from Abcam; Anti-SREBP1 (sc-13551, 1:200) and Anti-CPT1B (sc-393070, 1:500) from Santa Cruz Biotechnology

(10) Hyperuricemia contributes to glucose intolerance of hepatic inflammatory macrophages and impairs the insulin signaling pathway via IRS2-proteasome degradationFrontiers in ImmunologySeptember 13, 2022Hairong Zhao et al.(antibody, dilution, catalog number, company): anti-glucose transporter 4 (anti-GLUT4, 1:400, Cat# ab654, RRID: AB_305554, Abcam, USA), LAMP1 (1:200, Cat# ab24170, RRID: AB_775978, Abcam), and anti-F4/80 macrophage

(11) Stage-Specific Effect of Inositol Hexaphosphate on Cancer Stem Cell Pool during Growth and Progression of Prostate Tumorigenesis in TRAMP ModelCancersAugust 30, 2022Raina K, Kandhari K, Jain AK, Ravichandran K, Maroni P, Agarwal C, Agarwal R.System (#G7130) was purchased from Promega (Madison, WI, USA). Antibodies for VEGF (#ab46154), GLUT4 (#ab654), Sox-2 (#ab97959), and Oct-4 (#ab184665) were from Abcam (Cambridge, MA, USA). Antibody for iNOS (#NB300605)

(12) Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβNature communicationsJuly 25, 2022Zhou Q, Sun WW, Chen JC, Zhang HL, Liu J, Lin Y, Lin PC, Wu BX, An YP, Huang L, et al.(Danvers, Massachusetts, USA) . Antibodies against PAH (ab178430, 1:3000), IRβ (ab69508, 1:1000), GLUT4 (ab654, 1:3000) was purchased from Abcam (Cambridge, UK) . The antibodies against FARSA (18121-1-AP, 1:1000),

(13) Fluidic Flow Enhances the Differentiation of Placental Trophoblast-Like 3D Tissue from hiPSCs in a Perfused Macrofluidic DeviceFrontiers in Bioengineering and BiotechnologyJune 30, 2022Pengwei Deng, Kangli Cui, Yang Shi, Yujuan Zhu, Yaqing Wang, Xiaoguang Shao, Jianhua Qin(rabbit, 1:300, Abcam, ab53087), ZO-1 (rabbit, 1:100, Abcam, ab221547), and GLUT-4 (rabbit, 1:100, Abcam, ab654). The secondary antibodies were Alexa Fluor 488- and 594 conjugated anti-donkey (CST, 1:500). 2.8 Western Blot Analysis

(14) Development of a physiological insulin resistance model in human stem cell–derived adipocytesScience AdvancesJune 17, 2022Max Friesen, Andrew S. Khalil, M. Inmaculada Barrasa, Jacob F. Jeppesen, David J. Mooney, Rudolf JaenischAfter blocking, the cells were incubated in primary antibodies [anti-glucose transporter GLUT4 (Abcam, ab654; 1:500 dilution) or rabbit anti-C/EBPα (CCAAT Enhancer Binding Protein Alpha) (Cell Signaling Technology,

(15) 17β-Estradiol Treatment Improves Acetylcholine-Induced Relaxation of Mesenteric Arteries in Ovariectomized UC Davis Type 2 Diabetes Mellitus Rats in Prediabetic StateFrontiers in PhysiologyJune 17, 2022Md Rahatullah Razan, Farjana Akther, Rifat A. Islam, James L. Graham, Kimber L. Stanhope, Peter J. Havel, Roshanak Rahimian(Danvers, MA). Antibodies against NADPH oxidase-1 (NOX-1) (#ab131088) and glucose transporter-4 (GLUT-4) (#ab654) were obtained from Abcam (Cambridge, MA). All primary antibodies were diluted to 1:1,000 except for

(16) Increased fatty acid metabolism attenuates cardiac resistance to β-adrenoceptor activation via mitochondrial reactive oxygen species: A potential mechanism of hypoglycemia-induced myocardial injury in diabetesRedox biologyJune 1, 2022Huang L, Chen Z, Chen R, Lin L, Ren L, Zhang M, Liu L.acyl-CoA dehydrogenase (MCAD, Cat.55210-1-AP, 1:1000; Proteintech), glucose transporter 4 (GLUT4, Cat.ab654, 1:1000; Abcam), complex I (COX I, cat.19703-1-AP, 1:1000; Proteintech) and complex IV (COX IV, cat.ab14744,

(17) Methylene-bridge tryptophan fatty acylation regulates PI3K-AKT signaling and glucose uptakeCell reportsMarch 15, 2022Hu SH, He XD, Nie J, Hou JL, Wu J, Liu XY, Wei Y, Tang HR, Sun WX, Zhou SX, et al.Cell Signaling Technology, 4685), anti-PDK1 (1:100, Cell Signaling Technology, 5662), anti-GLUT4 (1:100, Abcam, ab654), mouse anti-E-cadherin (1:100, Cell Signaling Technology, 14472). The secondary antibodies (1:1000,

(18) VEGF alleviates lower limb ischemia in diabetic mice by altering muscle fiber typesExperimental and Therapeutic MedicineJanuary 31, 2022Lijing Jia, Peilin Zheng, Hongbo Wang, Lin Kang, Han Wu, Xiaobing Fucoactivator 1-α (PGC-1α; cat. no. ab54481, 1:1,000), anti-glucose transporter member (GLUT) 4 (cat. no. ab654, 1:1,000) antibodies were purchased from Abcam. The anti-PI3K (cat. no. 4292, 1:1,000), phosphorylated

(19) Alterations in Energy Metabolism, Mitochondrial Function and Redox Homeostasis in GK Diabetic Rat Tissues Treated with AspirinLife (Basel)January 12, 2022Annie John, Layla Amiri, Jasmin Shafarin, Saeed Tariq, Ernest Adeghate, Frank Christopher Howarth, Haider RazaCruz, CA, USA) and against heme oxygenase-1 (HO-1) (# ab13248) and glucose transporter-4 (Glut-4) (# ab654) from Abcam (Cambridge, UK). Kits for superoxide dismutase (SOD) (#ab65354) and glutamate dehydrogenase

(20) LRP1 mediates the IGF-1-induced GLUT1 expression on the cell surface and glucose uptake in Müller glial cellsScientific ReportsDecember 1, 2021Virginia Actis Dato, María Cecilia Sánchez, Gustavo Alberto Chiabrando(#ab182408) and mouse monoclonal anti-GLUT1 (#ab40084), rabbit anti-GLUT2 (#ab95256) and rabbit anti-GLUT4 (#ab654) were from Abcam (Cambridge, MA). Mouse monoclonal anti-APT1A1 (#M7-PB-E9) was from ThermoFisher Scientific

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11/3/2021Dependable GLUT4 Antibody Zewen JiangInvestigating insulin-dependent and insulin-independent signaling pathways under the context of obesity.

Anti-Glucose Transporter GLUT4 antibody from Abcam

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Anti-Glucose Transporter GLUT4 antibody from Abcam

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