Fig 1: p75NTR expression in human brain and platelets. (A) Healthy human cortex (C; 10 µg) and platelets lysates (platelets; 30 µg) were analyzed in denaturing and reducing conditions and blotted with antibodies raised against p75NTR. Left: molecular weight marker in kDa. Right: antibody catalog number. ß-actin was used as a loading control. (B) U251-MG cells and healthy human platelet lysates were either left untreated (untreated) or submitted to 37°C overnight in absence (Sham) or presence of the protein deglycosylation mix II (Deglyco). Membranes were blotted with the HPA004765 antibody against p75NTR ICD. Membranes were then stripped and reblotted for lysosomal-associated membrane protein 1 (LAMP-1) and CD41 as internal controls of enzymatic activity. (C) Human glioblastoma U87-MG cells and healthy human platelets isolated from whole blood were fixed or fixed and permeabilized. Cells were labeled with antibodies directed toward the p75NTR receptor and analyzed by flow cytometry. Results displayed are representative of (A) = 3 independent experiments and = 5 different platelet samples from different donors and (B, C) 3 independent experiments and 3 different donors for platelets samples.