Anti-Fibrillarin antibody - Nucleolar Marker ab5821 from Abcam

Product Specs
ItemAnti-Fibrillarin antibody - Nucleolar Marker
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab5821
Antibody TypePrimary
Quantity100 UG
IsotypeIgG
ClonalityPolyclonal
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot
HostRabbit
Conjugate/TagUnconjugated
Concentration0.5 mg/ml
Research AreaEpigenetics
Gene Namefbl
ReactivityDrosophila melanogaster, Human, Mouse
TargetFibrillarin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Chromosome-specific maturation of the epigenome in the Drosophila male germlineeLifeNovember 30, 2023James T Anderson, Steven Henikoff, Kami AhmadCell Signalling Technology C36B11 anti-H3-K27-trimethyl, Abcam ab109463 anti-H4-K16-acetyl, and Abcam ab5821 anti-fibrillarin. Imaging whole testes Testes from 1-day-old adult males were dissected and fixed

(2) NAD+ regulates nucleotide metabolism and genomic DNA replicationNature Cell BiologyNovember 13, 2023Sebastian Howen Nesgaard Munk, Joanna Maria Merchut-Maya, Alba Adelantado Rubio, Arnaldur Hall, George Pappas, Giacomo Milletti, MyungHee Lee, Lea Giørtz Johnsen, Per Guldberg, Jiri Bartek, et al.detection of BrUTP incorporation into nascent transcripts together with an anti-Fibrillarin antibody (Abcam ab5821, 1:500). Images were acquired on a LSM800 confocal microscope with a Plan-Apochromat 63x/1.4 numerical

(3) Affinity hierarchies and amphiphilic proteins underlie the co-assembly of nucleolar and heterochromatin condensatesResearch SquareNovember 9, 2023Srivarsha Rajshekar, Srivarsha Adame-Arana, Gaurav Adame-Arana, Gaurav Bajpai, Serafin Colmenares, Serafin Colmenares, Samuel SafranReagent or Resource,Source,Identifier Antibodies,, Rabbit anti-Fibrillarin,Abcam,ab5821 Mouse anti-H3K9me2,Abcam,ab8898 Mouse anti-Modulo,"Gift from Mellone Lab (gift of Jacques Pradel,

(4) Affinity hierarchies and amphiphilic proteins underlie the co-assembly of nucleolar and heterochromatin condensatesbioRxivSeptember 30, 2023Srivarsha Rajshekar, Omar Adame-Arana, Gaurav Bajpai, Kyle Lin, Serafin Colmenares, Samuel Safran, Gary H KarpenReagent or Resource,Source,Identifier Antibodies,, Rabbit anti-Fibrillarin,Abcam,ab5821 Mouse anti-H3K9me2,Abcam,ab8898 Mouse anti-Modulo,"Gift from Mellone Lab (gift of Jacques Pradel,

(5) Human nucleolar protein SURF6/RRP14 participates in early steps of pre-rRNA processingPLOS ONEJuly 14, 2023Anastasiia Moraleva, Alexander Deryabin, Maria Kordyukova, Mikhail Polzikov, Kseniya Shishova, Kira Dobrochaeva, Yury Rubtsov, Maria Rubtsova, Olga Dontsova, Olga Zatsepinaantibodies to SURF6 [21] (diluted 1:100) and rabbit antibodies to Fibrillarin (diluted 1:200; Abcam, ab5821) for 1h at room temperature. Then washed for 3x5 min in PBS and stained with a mixture of antibodies

(6) Centromere proteins are asymmetrically distributed between newly divided germline stem and daughter cells and maintain a balanced niche in Drosophila malesMolecular Biology of the CellMay 1, 2023Antje M. Kochendoerfer, Rachel S. Keegan, Elaine M. Dunleavy1:700), mouse anti-1B1 (Hts; DSHB; 1:100), mouse anti-BAM (DSHB; 1:50), mouse anti-FIBRILLARIN (Abcam; ab5821; 1:500), mouse anti-HA (ThermoFisher; 26183; 1:500), mouse anti-gamma-tubulin (Abcam; ab44928; 1:500),

(7) DDX47, MeCP2, and other functionally heterogeneous factors protect cells from harmful R loopsCell reportsMarch 28, 2023Marchena-Cruz E, Camino LP, Bhandari J, Silva S, Marqueta-Gracia JJ, Amdeen SA, Guillén-Mendoza C, García-Rubio ML, Calderón-Montaño JM, Xue X, et al.Anti-FLAG,Abcam,Cat# ab1257;RRID: AB_299216 Polyclonal antibody Rabbit anti-Fibrillarin,Abcam,Cat# ab5821;RRID:AB_2105785 Monoclonal antibody anti-Histidine,Sigma,Cat# GE27-4710-01 Polyclonal antibody Rabbit

(8) A Dynamic rRNA Ribomethylome Drives Stemness in Acute Myeloid LeukemiaCancer discoveryFebruary 6, 2023Zhou F, Aroua N, Liu Y, Rohde C, Cheng J, Wirth AK, Fijalkowska D, Göllner S, Lotze M, Yun H, et al.in PBS containing 0.5% (v/v) Tween-20 with 5% milk powder. Primary antibodies against anti-FBL (cat. ab5821, Abcam), anti-V5 tag (cat. ab9116, Abcam), anti–β-Actin (cat. ab6276, Abcam), anti-PRPF39 (cat. GTX104949,

(9) Expression of RNA polymerase I catalytic core is influenced by RPA12PloS oneJanuary 1, 2023Ford BL, Wei T, Liu H, Scull CE, Najmi SM, Pitts S, Fan W, Schneider DA, Laiho M.monoclonal D10 1:100; sc-393406 Santa Cruz Biotechnology) and fibrillarin (rabbit polyclonal 1:500; ab5821 Abcam). Secondary antibodies used were Alexa 488 and Alexa 594-conjugated anti-mouse and anti-rabbit

(10) Nucleophagy delays aging and preserves germline immortalityNat AgingJanuary 1, 2023Papandreou ME, Konstantinidis G, Tavernarakis N.anti-p62/SQSTM1 (catalog no. GP62-C; Progen); rabbit anti-Syne-1 or rabbit anti-fibrillarin (catalog no. ab5821; Abcam). Coverslips were then washed with PBS, followed by secondary antibody staining for 1 h at room

(11) MALAT1-dependent hsa_circ_0076611 regulates translation rate in triple-negative breast cancerCommunications BiologyDecember 1, 2022Chiara Turco, Gabriella Esposito, Alessia Iaiza, Frauke Goeman, Anna Benedetti, Enzo Gallo, Theodora Daralioti, Letizia Perracchio, Andrea Sacconi, Patrizia Pasanisi, et al.(Cell Signaling: #2469), anti-SRSF1 (Santa Cruz: SF2/ASF96 sc-33652) and anti-Fibrillarin (Abcam: FBL ab5821) were used. ChIRP assay of circ_0076611, RNAseq and bioinformatic analysis ChIRP was performed as

(12) Low level of Fibrillarin, a ribosome biogenesis factor, is a new independent marker of poor outcome in breast cancerBMC CancerDecember 1, 2022Flora Nguyen Van Long et al.immunohistochemical staining FBL immunohistochemistry (IHC) was performed using two different lots of FBL antibody for CLB-1 (ab5821 lot GR1979-001, Abcam) and IGR-1 series (ab5821 lot GR253838-1, Abcam). FBL staining corresponds to

(13) HITS-CLIP analysis of human ALKBH8 reveals interactions with fully processed substrate tRNAs and with specific noncoding RNAsRNADecember 1, 2022Ivana Cavallin, Marek Bartosovic, Tomas Skalicky, Praveenkumar Rengaraj, Martin Demko, Martina Christina Schmidt-Dengler, Aleksej Drino, Mark Helm, Stepanka VanacovaSigma Aldrich F3165), anti-tubulin (1:10,000, Sigma Aldrich T6074), anti-fibrillarin (1:1000, Abcam ab5821), anti-ALKBH8 (1:500, Abcam ab113512). Washes and incubation of HRP-conjugated secondary antibodies

(14) A Spontaneous Inversion of the X Chromosome Heterochromatin Provides a Tool for Studying the Structure and Activity of the Nucleolus in Drosophila melanogasterCellsDecember 1, 2022Tatyana D. Kolesnikova, Mikhail S. Klenov, Alina R. Nokhova, Sergey A. Lavrov, Galina V. Pokholkova, Veit Schubert, Svetlana V. Maltseva, Kevin R. Cook, Michael J. Dixon, Igor F. Zhimulevwere performed according to the protocol described in [38]. Rabbit polyclonal anti-Fibrillarin (Abcam, ab5821, 1:5000) or guinea pig anti-Udd (1:300, [46], kindly provided by M. Buszczak) primary antibodies and

(15) AATF /Cheâ€1 localizes to paraspeckles and suppresses Râ€loops accumulation and interferon activation in Multiple MyelomaThe EMBO JournalNovember 17, 2022Tiziana Bruno, Giacomo Corleone, Valeria Catena, Clelia Cortile, Francesca De Nicola, Francesca Fabretti, Svitlana Gumenyuk, Francesco Pisani, Andrea Mengarelli, Claudio Passananti, et al.anti‐αTubulin (DM1A) (WB 1:500),Calbiochem,Cat# CP06 Rabbit Polyclonal anti‐Fibrillarin (IF 1:100),Abcam,Cat# ab5821 Alexa Fluor 594‐anti‐Rabbit (IF 1:500),ThermoFisher,Cat# A11037 Alexa Fluor 594‐anti‐Mouse (IF 1:500),ThermoFisher,Cat#

(16) Control of ribosomal RNA synthesis by hematopoietic transcription factorsMolecular cellOctober 20, 2022Antony C, George SS, Blum J, Somers P, Thorsheim CL, Wu-Corts DJ, Ai Y, Gao L, Lv K, Tremblay MG, et al.manufacturer protocol. Precleared cell extract was incubated with 5 µg of anti-Fibrillarin (Abcam, #ab5821), or anti-FLAG-M2 (Sigma, #F1804) antibodies, or Rabbit Polyclonal IgG (Invitrogen, #02–6102), or Mouse

(17) Epithelial cell size dysregulation in human lung adenocarcinomaPLOS ONEOctober 6, 2022Clifford W. Sandlin, Song Gu, Jun Xu, Charuhas Deshpande, Michael D. Feldman, Matthew C. GoodA+C),Rabbit,Monoclonal/-,Abcam,ab108595,1° IF / 1:200 Proprietary synthetic Fibrillarin peptide (Fibrillarin),Rabbit,Polyclonal/-,Abcam,ab5821,1° IF / 1:200 Human SON/DBP5 1463–1598 (SON),Rabbit,Polyclonal/-,Abcam,ab121759,1° IF / 1:200 "Keratin,

(18) Adeno-associated virus type 2 (AAV2) uncoating is a stepwise process and is linked to structural reorganization of the nucleolusPLOS PathogensJuly 11, 2022Sereina O. Sutter, Anouk Lkharrazi, Elisabeth M. Schraner, Kevin Michaelsen, Anita Felicitas Meier, Jennifer Marx, Bernd Vogt, Hildegard Büning, Cornel Fraefel1:10), anti-AAV2 Rep (Fitzgerald Industries, 10R-A111A, dilution for IF 1:10), anti-fibrillarin (Abcam ab5821; dilution for IF 1:200; dilution for Western blotting [WB] 1:650), anti-cyclin A (Santa Cruz sc-751,

(19) Vulnerability of HIF1α and HIF2α to damage by proteotoxic stressorsToxicology and Applied PharmacologyJune 15, 2022Lauren M. Meyers, Casey Krawic, Michal W. Luczak, Anatoly ZhitkovichImmunoBlot polyvinylidene difluoride membranes (1620177, Bio-Rad). Primary antibodies: fibrillarin (ab5821) and phospho-S326-HSF1 (ab76076) from Abcam; HIF1α (610958) from BD Biosciences; HA-tag (3724), HIF1α

(20) Promyelocytic leukemia nuclear body-like structures can assemble in mouse oocytesBiology OpenJune 15, 2022Osamu Udagawa, Ayaka Kato-Udagawa, Seishiro Hiranotelangiectasia (CREST) human autoantibody (HCT-0100: Immuno Vision, Springdale, USA), anti-fibrillarin (ab5821), anti-SUMO-conjugating enzyme (Ubc9), anti-proteasome 20S alpha 1+2+3+5+6+7 (20S) (Abcam, Cambridge,

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Anti-Fibrillarin antibody - Nucleolar Marker from Abcam

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Anti-Fibrillarin antibody - Nucleolar Marker from Abcam

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