Anti-AANAT antibody ab3505 from Abcam

Product Specs
ItemAnti-AANAT antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab3505
Antibody TypePrimary
ClonalityPolyclonal
Quantity50 UG
ApplicationsWestern blot
HostRabbit
IsotypeIgG
Conjugate/TagUnconjugated
FormatTotal anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific amino-terminal IgG was subsequently isolated on resin-coupled phosphopeptide
Concentration1 mg/ml
Gene NameAANAT
ReactivityRat
TargetSerotonin N-acetyltransferase

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Website: http://www.abcam.com

(1) The Uterine Melatonergic Systems of AANAT and Melatonin Membrane Receptor 2 (MT2) Are Essential for Endometrial Receptivity and Early Implantation in MiceInternational journal of molecular sciencesApril 12, 2023Ma X, Wang J, Wang L, Yan L, Liu Y, Ma W, Ji P, Zhang L, Liu G.hydrogen peroxide in the dark for 15 min and washed with PBS. The anti-AANAT antibody (1:200 dilution; ab3505; Abcam, Cambridge, UK), anti-estrogen receptor alpha antibody (1:200 dilution; ab32063; Abcam, Cambridge,

(2) Melatonin Supplementation during the Late Gestational Stage Enhances Reproductive Performance of Sows by Regulating Fluid Shear Stress and Improving Placental Antioxidant CapacityAntioxidants (Basel)March 10, 2023Likai Wang, Laiqing Yan, Qi Han, Guangdong Li, Hao Wu, Xiao Ma, Mengmeng Zhao, Wenkui Ma, Pengyun Ji, Ran Zhang, et al.were purchased from Beijing North Biotechnology Research Institute Co., Ltd. Antibodies against AANAT (ab3505), MTNR1A (ab203038) and MTNR1B (ab203346) were purchased from Abcam (Cambridge, MA). The secondary antibody

(3) Circ-ERC2 Is Involved in Melatonin Synthesis by Regulating the miR-125a-5p/MAT2A AxisInternational journal of molecular sciencesDecember 7, 2022Guo HX, Zheng Y, Zhao GK, Wang HQ, Yu S, Gao F, Zhang JB, Zhang YH, Yuan B.2118S), MAT2A + MAT1A antibody was from Abcam (1:1000, ab177484), AANAT antibody was from Abcam (1:1000, ab3505), and ASMT antibody was from GeneTex (1:1000, GTX130292). The membrane was then washed three times with

(4) Prolonged Administration of Melatonin Ameliorates Liver Phenotypes in Cholestatic Murine ModelCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Ceci L, Chen L, Baiocchi L, Wu N, Kennedy L, Carpino G, Kyritsi K, Zhou T, Owen T, Kundu D, et al.Antibodies,Application,Description,Catalog number,Company AANAT,IHC,Rabbit polyclonal,ab3505,"Abcam (Cambridge, UK)" ARNTL,IF,Rabbit polyclonal,PA1-523,"Invitrogen (Waltham, MAMassachusetts, US)" B-actin,WB,Mouse

(5) Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT PathwayNutrientsDecember 28, 2021Lijin Song, Meibo He, Qinghua Sun, Yujing Wang, Jindong Zhang, Yuan Fang, Shuangjiang Liu, Liping Duan°C for 1 h, and then incubated overnight at 4 °C with primary antibodies, including AANAT (1:1000, #ab3505, Abcam, Cambridge, UK), CREB (1:1000, #9197, Cell Signaling Technology, Danvers, MA, USA), phosphorylated

(6) Melatonin delays ovarian aging in mice by slowing down the exhaustion of ovarian reserveCommunications BiologyDecember 1, 2021Chan Yang et al.For immunohistochemistry, the sections were incubated with rabbit polyclonal antibodies (SNAT: 1 : 100; ab3505, Abcam; PCNA: 1 : 100, GT2253, Genetech, Shanghai, China) overnight at 4 °C; then, the sections were

(7) Melatonin Alleviates the Suppressive Effect of Hypoxanthine on Oocyte Nuclear Maturation and Restores Meiosis via the Melatonin Receptor 1 (MT1)-Mediated PathwayFrontiers in Cell and Developmental BiologyApril 15, 2021Jing Wang et al.PBS for 20 min, and 0.3% BSA in PBS for 1 h and then incubated with anti-AANAT antibody (1:100 dilution; ab3505; Abcam), anti-MT1 antibody (1:200 dilution; ab203038; Abcam), and antiMT2 antibody (1:200 dilution;

(8) Increased Expression of Colonic Mucosal Melatonin in Patients with Irritable Bowel Syndrome Correlated with Gut DysbiosisGenomics, Proteomics & BioinformaticsFebruary 17, 2021Ben Wang, Shiwei Zhu, Zuojing Liu, Hui Wei, Lu Zhang, Meibo He, Fei Pei, Jindong Zhang, Qinghua Sun, Liping Duan250 mA for 90 min. The membranes were incubated with rabbit anti-AANAT antibody (1:1000; catalog No. ab3505, Abcam) overnight at 4 °C. β-Actin was chosen as the internal reference protein. The primary antibodies

(9) Loading-induced antitumor capability of murine and human urineFASEB journal : official publication of the Federation of American Societies for Experimental BiologyJune 1, 2020Wu D, Fan Y, Liu S, Woollam MD, Sun X, Murao E, Zha R, Prakash R, Park C, Siegel AP, et al.Western blotting, shRNA transfection, ELISA, and protein array analysis We used antibodies against AANAT (ab3505), CD105 (ab54338) (Abcam, Cambridge, MA, USA), Lrp5 (5731s), Lrp6 (3395T), Runx2 (8486s), Snail (3879s)

(10) A study of the mechanism of small-molecule soybean-protein-derived peptide supplement to promote sleep in a mouse modelRSC AdvancesMarch 16, 2020Guofu Yi, Bushra Safdar, Yihao Zhang, You Li, Xinqi Liupurchased from Biologicals Novus (Litton, Colorado, USA). Antibodies against TPH (ab52954), AANAT (ab3505), MT1 (ab87639), MT2 (ab56308) were obtained from Abcam (Cambridge, UK). 2.2 Molecular weight distribution

(11) Pinealectomy or light exposure exacerbates biliary damage and liver fibrosis in cholestatic rats through decreased melatonin synthesisBiochimica et Biophysica Acta (BBA) - Molecular Basis of DiseaseJune 1, 2019Lixian Chen et al.Louis, MO) unless otherwise indicated. The antibodies for cytokeratin-19 (CK-19, ab 52625), AANAT (ab3505), MT1 (ab203038), MT2 (ab203346), cyclin-dependent kinase inhibitor 1A (p16, ab189043), CLOCK (ab3517),

(12) Melatonin synthesis in the human ciliary body triggered by TRPV4 activation: Involvement of AANAT phosphorylationExperimental Eye ResearchJune 23, 2017Hanan Awad Alkozi, María J. Perez de Lara, Jesús Pintor

(13) TRPV4 Stimulation Induced Melatonin Secretion by Increasing Arylalkymine N-acetyltransferase (AANAT) Protein LevelInternational Journal of Molecular SciencesApril 1, 2017Hanan Awad Alkozi, Maria J. Perez de Lara, Juan Sánchez-Naves, Jesús Pintorh at room temperature. Then, the primary antibody rabbit anti-serotonin N-acetyltrasnferase (AANAT, ab3505, Abcam, Cambrigde, UK) was incubated at a 1:500 dilution at 4 °C overnight. Sections were washed in

(14) Homeostatic Plasticity Mediated by Rod-Cone Gap Junction Coupling in Retinal Degenerative Dystrophic RCS RatsFrontiers in cellular neuroscienceJanuary 1, 2017Hou B, Fu Y, Weng C, Liu W, Zhao C, Yin ZQ.X-100 for 15 min and then blocked in 5% goat serum for 1 h. Primary antibodies against AANAT (1:1,000, ab3505, Abcam), p-AANAT (phospho T29, 1:1,000, ab3439, Abcam) were diluted in blocking buffer, and the samples

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Anti-AANAT antibody from Abcam

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Anti-AANAT antibody from Abcam

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