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Anti-SOX9 antibody
Anti-SOX9 antibody from Abcam
Be the first to write a review!
Citations:
(66)
Supplier Page
Supplier Page from
Abcam for
Anti-SOX9 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-SOX9 antibody
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab3697
Antibody Type
Primary
Clonality
Polyclonal
Host
Rabbit
Quantity
50 UG
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Isotype
IgG
Conjugate/Tag
Unconjugated
Concentration
0.2 mg/ml
Gene Name
Sox9
Reactivity
Human, Mouse
Target
SOX9
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (66)
(1)
Spidroin striped micropattern promotes chondrogenic differentiation of human Wharton’s jelly mesenchymal stem cells
Scientific Reports
December 1, 2022
Anggraini Barlian, Dinda Hani’ah Arum Saputri, Adriel Hernando, Candrani Khoirinaya, Ekavianty Prajatelistia, Hutomo Tanoto
antibody with 3% Bovine Serum Albumin (BSA, in PBST) for 60 min. The primary antibody against SOX9 (
ab3697
,
Abcam
) was then added to the samples and incubated 4 °C overnight. The samples were washed with PBS
(2)
Cyclic uniaxial mechanical load enhances chondrogenesis through entraining the molecular circadian clock
Journal of pineal research
November 1, 2022
Vágó J, Katona É, Takács R, Dócs K, Hajdú T, Kovács P, Zákány R, van der Veen DR, Matta C.
polyclonal,
1:25
PER3
,
ab177482
,
Abcam
,Rabbit polyclonal,
1:25
RUNX2
,
8486S
,
Cell Signalling
Technology,Rabbit monoclonal,
1:25
SOX9,
ab3697
,
Abcam
,Rabbit polyclonal,
1:25
GAPDH,
ab9485
,
Abcam
,Rabbit polyclonal,
1:25
Antibodies and their dilutions
(3)
Adaptation of the protein translational apparatus during ATDC5 chondrogenic differentiation
Non-coding RNA Research
June 1, 2022
Mandy M.F. Steinbusch, Guus G.H. van den Akker, Andy Cremers, Adhiambo M.A. Witlox, Heleen M. Staal, Mandy J. Peffers, Lodewijk W. van Rhijn, Marjolein M.J. Caron, Tim J.M. Welting
monoclonal anti-UBF1 (F-9,
Santa Cruz
#
SC-13125
;
1:250
dilution), rabbit polyclonal anti-Sox9 (
Abcam
#
AB3697
;
1:100
dilution) and
mouse
monoclonal anti-
Puromycin
(12D10,
Merck Millipore
, Billerica, MA, USA, #
MABE343
;
(4)
Hypermethylation of miR-145 promoter-mediated SOX9-CLDN8 pathway regulates intestinal mucosal barrier in Crohn's disease
EBioMedicine
February 1, 2022
Zhuang X, Chen B, Huang S, Han J, Zhou G, Xu S, Chen M, Zeng Z, Zhang S.
Pierce Magnetic ChIP Kit
(
Thermo Fisher Scientific
, Waltham, MA, USA) with an anti-
SOX9
antibody (ChIP Grade;
Abcam
, Cat#
ab3697
, RRID: AB_304012) according to the manufacturer's instructions. ChIP-qPCR assay was carried out using
(5)
A low dose cell therapy system for treating osteoarthritis: In vivo study and in vitro mechanistic investigations
Bioactive Materials
January 1, 2022
Bin Wang, Wei Liu, Jiao Jiao Li, Senlin Chai, Dan Xing, Hongsheng Yu, Yuanyuan Zhang, Wenjin Yan, Zhihong Xu, Bin Zhao,
et al.
chondrocytes
and MSCs
Protein expression
levels of type II collagen (
SC-52658
,
Santa Cruz
, USA), SOX9 (
ab3697
,
Abcam
, USA), and GAPDH in co-cultured
chondrocytes
and MSCs were measured using
Western blot
. Cytosolic
(6)
Circular RNA mitochondrial translation optimization 1 homologue (CircMTO1) induced by zinc finger protein 460 (ZNF460) promotes oral squamous cell carcinoma progression through the microRNA miR-320a / alpha thalassemia/mental retardation, X-linked (ATRX) axis
Bioengineered
December 20, 2021
Chen Zou, Xia Li, Xiaozhi Lv, Siyuan Wu, Jing Song, Zhe Tang, Hailing Luo, Haigang Wei, Yilong Ai
glycine. Then, the lysates were sonicated, digested, and incubated with ChIP-grade ZNF460 antibody (#
ab3697
,
Abcam
, Cambridge, UK) and negative control IgG at 4°C overnight. The next day, protein A agarose was
(7)
Runx1 protects against the pathological progression of osteoarthritis
Bone research
December 7, 2021
Zhou C, Cui Y, Yang Y, Guo D, Zhang D, Fan Y, Li X, Zou J, Xie J.
slice pretreatment was the same as that
for immunohistochemistry
. The primary antibodies included SOX9 (
ab3697
,
Abcam
,
1:200
dilution), Collagen X (
ab58632
,
Abcam
,
1:200
dilution), and MMP13 (
ab39012
,
Abcam
,
1:200
(8)
Effects of maternal consumption of morphine on rat skeletal system development
BMC Musculoskeletal Disorders
December 1, 2021
Maryam Saeidinezhad, Vahid Razban, Hosein Safizadeh, Massood Ezzatabadipour
from
Abcam
company (Cat#
ab34712
) and SRY-box transcription factor 9 (SOX9) from
Abcam
company (Cat#
ab3697
), as chondrogenic markers, were purchased from Abcam and Millipore companies [22, 33]. In
IHC
cell’s
(9)
Roles for B[a]P and FICZ in subchondral bone metabolism and experimental temporomandibular joint osteoarthritis via the AhR/Cyp1a1 signaling axis
Scientific Reports
December 1, 2021
Yuri Yoshikawa, Takashi Izawa, Yusaku Hamada, Hiroko Takenaga, Ziyi Wang, Naozumi Ishimaru, Hiroshi Kamioka
Dallas, TX, USA), aggrecan (
ab36861
;
Abcam
, Cambridge, United Kingdom),
Col2a1
(
ab21291
;
Abcam
), Sox9 (
ab3697
;
Abcam
), cleaved
caspase-3
(#
9661
;
Cell Signaling Technology
, Danvers, MA, USA), and AhR (
BML-SA210
;
(10)
Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis
Stem Cell Research & Therapy
December 1, 2021
Ning Li, Yichi Zhang, Narayan Nepal, Guoqing Li, Ningning Yang, Haoyuan Chen, Qiuchi Lin, Xuechun Ji, Sijia Zhang, Shizhu Jin
(
1:200
,
ab14389
,
Abcam
), anti-Musashi1 (
1:200
, c-135,721, Santa Cruz Biotechnology), anti-Sox9 (
1:100
,
ab3697
,
Abcam
) and anti-PCNA antibodies (
1:200
,
ab92552
,
Abcam
) at 4 °C overnight. Next, the sections were
(11)
NF-κB activation impedes the transdifferentiation of hypertrophic chondrocytes at the growth plate of mouse embryos in diabetic pregnancy
Journal of orthopaedic translation
November 1, 2021
Liu X, Qian F, Fan Q, Lin L, He M, Li P, Cai H, Ma L, Cheng X, Yang X.
was performed at 4 °C overnight, using monoclonal primary antibodies against: Sox9 (
1:200
,
Abcam
,
ab3697
, Cambridge, MA),
Runx2
(
1:200
,
Abcam
,
ab114133
, USA), Osterix (
1:300
,
Abcam
,
ab22552
, UK),
Bmp2
(
1:100
,
(12)
MIR106A-5p
upregulation suppresses autophagy and accelerates malignant phenotype in nasopharyngeal carcinoma
Autophagy
July 1, 2021
Zhu Q, Zhang Q, Gu M, Zhang K, Xia T, Zhang S, Chen W, Yin H, Yao H, Fan Y,
et al
.
antibodies against
EGR1
(
Santa Cruz Biotechnology
,
sc-101033
; 5 µg; polyclonal
mouse
), SOX9 (
Abcam
,
ab3697
; 5 µg; polyclonal rabbit), or an isotype control IgG (Millipore; 345701, 1 µg; mouse IgG).
PCR
was performed
(13)
CAP2 contributes to tumorigenesis in gastric cancer by targeting transcription factor SOX9
Journal of Gastrointestinal Oncology
May 20, 2021
Ying Wan, Shengkui Qiu, Lei Yin, Xuesong Gao, Yasu Jiang, Shichun Feng, Chong Tang
2 h with 5% non-fat milk and then incubated with primary antibodies
CAP2
(
ab236590
,
Abcam
)and SOX9 (
ab3697
,
Abcam
) at 4 ℃ overnight. Then,
horseradish
peroxidase-conjugated secondary antibodies were used to
(14)
Stromal Galectin-1 Promotes Colorectal Cancer Cancer-Initiating Cell Features and Disease Dissemination Through SOX9 and β-Catenin: Development of Niche-Based Biomarkers
Frontiers in oncology
January 1, 2021
Peng KY, Jiang SS, Lee YW, Tsai FY, Chang CC, Chen LT, Yen BL.
No.
ABE993
;
Millipore
), E-cadherin (
1:1,000
; Cat. No.
MAB3199
;
Millipore
), SOX9 (
1:1,000
; Cat. No.
ab3697
;
Abcam
),
GAPDH
(
1:8,000
; Cat. No.
14-9523-80
;
eBioscience
, CA, USA), α-Tubulin (
1:8,000
; Cat. No.
14-4502-82
;
(15)
Noncoding mutations target cis-regulatory elements of the FOXA1 plexus in prostate cancer
Nature Communications
December 1, 2020
Stanley Zhou et al.
(FOXA1 5 µg,
Abcam
,
ab23738
; AR 5 µg, Abcam, ab1083241;
HOXB13
5 µg,
Abcam
,
ab201682
; SOX9 5 µg,
Abcam
,
ab3697
; GATA2 5 µg,
Abcam
,
ab22849
; FOXP1 5 µg,
Abcam
,
ab16645
; NKX3.1 10 µl, Cell Signalling Technology, #83700)
(16)
CircSERPINE2 weakens IL-1β-caused apoptosis and extracellular matrix degradation of chondrocytes by regulating miR-495/TGFBR2 axis
Bioscience Reports
November 27, 2020
Qingpu Zhang, Xiaomiao Qiao, Wenwei Xia
non-fat milk, and then interacted with primary antibodies anti-Sry-type high-mobility-group box 9 (SOX9) (
ab3697
,
1:1000
dilution,
Abcam
),
anti-collagen type II
α 1 (COL2A1) (
AB761
,
1:100
dilution,
Sigma–Aldrich
),
(17)
CYP19A1 (aromatase) dominates female gonadal differentiation in chicken (
Gallus gallus
) embryos sexual differentiation
Bioscience Reports
October 30, 2020
Kai Jin, Qisheng Zuo, Jiuzhou Song, Yani Zhang, Guohong Chen, Bichun Li
temperature for 1 h. Then slides were stained with anti-SOX9 antibody (
1:500
, Abcam, Cambridge, U.K.,
ab3697
) or anti-aromatase antibody (
1:250
,
Abcam
, Cambridge, U.K., ab139492) overnight at 4◦ C. After three washes
(18)
JNK-mediated disruption of bile acid homeostasis promotes intrahepatic cholangiocarcinoma
Proceedings of the National Academy of Sciences
July 14, 2020
Elisa Manieri et al.
with antibodies against
PCNA
(biotinylated from
Thermofisher
MS-106-B; RRID:AB_64272), SOX9 (
Abcam
ab3697
; RRID:AB_304012), glutamine synthetase (
Abcam
ab73593
; RRID:AB_2247588), cytokeratin 19 (
Abcam
ab15463
;
(19)
SOX9 interacts with FOXC1 to activate MYC and regulate CDK7 inhibitor sensitivity in triple-negative breast cancer
Oncogenesis
May 12, 2020
Lin Tang, Juan Jin, Kun Xu, Xin Wang, Jinhai Tang, Xiaoxiang Guan
(#
7074
), mouse IgG (#
7076
) and
GAPDH
(#
5174
) were obtained from
Cell Signaling Technologies
; Anti-SOX9 (
ab3697
), anti-MYC (
ab32072
), anti-RUNX1 (
ab138377
) anti-Snail (
ab53519
), anti-E-cadherin (
ab133597
), anti-N-cadherin
(20)
A kinome-wide screen identifies a CDKL5-SOX9 regulatory axis in epithelial cell death and kidney injury
Nature communications
April 21, 2020
Kim JY, Bai Y, Jayne LA, Hector RD, Persaud AK, Ong SS, Rojesh S, Raj R, Feng MJHH, Chung S,
et al
.
M2 Affinity Gel, Sigma-Aldrich), anti-CDKL5 (
Millipore
,
MABS1132
), and anti-SOX9 antibodies (
Abcam
,
ab3697
). Invitrogen Bis-tris gradient mini or midi-gels were used
for western blot
analysis, followed by detection
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Anti-SOX9 antibody from Abcam
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