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Anti-Clathrin heavy chain antibody [X22]
Anti-Clathrin heavy chain antibody [X22] from Abcam
Be the first to write a review!
Citations:
(70)
Supplier Page
Supplier Page from
Abcam for
Anti-Clathrin heavy chain antibody [X22]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Clathrin heavy chain antibody [X22]
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab2731
Antibody Type
Primary
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Clone
X22
Clonality
Monoclonal
Quantity
100 UL
Isotype
IgG1
Host
Mouse, Nude mouse
Conjugate/Tag
Unconjugated
Concentration
6 mg/ml
Gene Name
CLTC
Reactivity
Human, Xenopus laevis
Target
Clathrin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (70)
(1)
Loss of Tumour Suppressor TMEM127 Drives RET-mediated Transformation Through Disrupted Membrane Dynamics
bioRxiv
December 19, 2023
Timothy J. Walker, Eduardo Reyes-Alvarez, Brandy D. Hyndman, Michael G. Sugiyama, Larissa C.B. Oliveira, Aisha N. Rekab, Mathieu J.F. Crupi, Rebecca Cabral-Dias, Qianjin Guo, Patricia L.M. Dahia,
et al.
USA). The anti-γ-Tubulin (#
T6557
) antibody was from
Sigma Aldrich
. Clathrin Heavy Chain antibodies (#
ab2731
and #
ab21679
),
RET
(#
ab134100
), and Alpha-adaptin (
ab2730
) were from
Abcam
(Cambridge, UK). The
TMEM127
(2)
P21-activated kinase 1 (PAK1)-mediated cytoskeleton rearrangement promotes SARS-CoV-2 entry and ACE2 autophagic degradation
Signal Transduction and Targeted Therapy
October 9, 2023
Ming Liu, Bingtai Lu, Yue Li, Shuofeng Yuan, Zhen Zhuang, Guangyu Li, Dong Wang, Liuheyi Ma, Jianheng Zhu, Jinglu Zhao,
et al.
(
CST
, Cat#
2606
), anti-pPAK1(T423) (CST, Cat#
2606
), anti-human PAK1(
CST
, Cat#
2602
), anti-CHLC(
Abcam
, Cat#
ab2731
) (
CST
, Cat #
4796
), anti-CK2α (
CST
, Cat #
2656S
), anti-SARS-CoV-2 spike protein (
SinoBiological
, Cat#
40591-MM42
). The
(3)
MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling
Nature Communications
October 4, 2023
He Cao, Panpan Yang, Jia Liu, Yan Shao, Honghao Li, Pinglin Lai, Hong Wang, Anling Liu, Bin Guo, Yujin Tang,
et al.
rabbit polyclonal anti-clathrin (
1:200
,
ab21679
,
Abcam
),
mouse
monoclonal anti-clathrin (
1:200
, X22,
ab2731
,
Abcam
),
mouse
monoclonal anti-RAB5 (
1:200
, 3A4,
ab66746
,
Abcam
),
mouse
monoclonal
anti-RAB7
(
1:200
,
(4)
α-Synuclein colocalizes with AP180 and affects the size of clathrin lattices
Journal of Biological Chemistry
September 1, 2023
Karina J. Vargas, P.L. Colosi, Eric Girardi, Jae-Min Park, Leah E. Harmon, Sreeganga S. Chandra
reconstitution mixture. For triple immunostaining for PI(4,5)P2, primary antibodies consisted of clathrin
Abcam
ab2731
, α-synuclein Cell Signaling D37A6, PI(4,5)P2
Echelon Biosciences
Z-P045
; secondary antibodies consisted
(5)
Uric acid regulates α-synuclein transmission in Parkinsonian models
Frontiers in Aging Neuroscience
August 28, 2023
Yu Jin Shin, Yeon Ju Kim, Ji Eun Lee, Yi Seul Kim, Jung Wook Lee, HyeonJeong Kim, Jin Young Shin, Phil Hyu Lee
used for
staining
are as follows: rabbit anti-α-syn (
Abcam
,
ab138501
),
mouse
anti-clathrin (
Abcam
,
ab2731
), rabbit anti-Rab5 (
Abcam
,
ab18211
).
Immunofluorescence
labeling
was carried out by incubating cells
(6)
Single-frame deep-learning super-resolution microscopy for intracellular dynamics imaging
Nature communications
May 18, 2023
Chen R, Tang X, Zhao Y, Shen Z, Zhang M, Shen Y, Li T, Chung CHY, Zhang L, Wang J,
et al
.
Anti-EGFR antibody (R-1) (SCBT, sc-101) for EGFR and
1:200
anti-clathrin heavy chain antibody (
Abcam
,
ab2731
) for clathrin) in blocking buffer. After
incubation
with primary antibodies, the coverslips were rinsed
(7)
The TGEV Membrane Protein Interacts with HSC70 To Direct Virus Internalization through Clathrin-Mediated Endocytosis
Journal of Virology
April 27, 2023
Zhaoyang Ji, Hui Dong, Ruixue Jiao, Xiaoyuan Zhu, Hongyan Shi, Jianfei Chen, Da Shi, Jianbo Liu, Zhaoyang Jing, Jialin Zhang,
et al.
Anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (
ab8245
), anti-HSC70 (
ab1427
,
ab69558
), and anti-CLHC (
ab2731
) antibodies were purchased from
Abcam
. Alexa Fluor 488-labeled
rabbit anti-mouse IgG (H+L
), Alexa Fluor
(8)
Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs
Nature Communications
April 26, 2023
Filip Mihalič, Leandro Simonetti, Girolamo Giudice, Marie Rubin Sander, Richard Lindqvist, Marie Berit Akpiroro Peters, Caroline Benz, Eszter Kassa, Dilip Badgujar, Raviteja Inturi,
et al.
at 4 °C with primary antibodies
goat
-anti-FLAGtag (
ab1257
,
Abcam
) (
1:1000
) and
mouse
-anti-clathrin (
ab2731
,
Abcam
) (
1:200
) diluted in blocking buffer. The slides were washed 3 times 10 min in TBS plus 0,05%
(9)
Nanoparticle-mediated targeting of the fusion gene RUNX1/ETO in t(8;21)-positive acute myeloid leukaemia
Leukemia
April 1, 2023
Issa H, Swart LE, Rasouli M, Ashtiani M, Nakjang S, Jyotsana N, Schuschel K, Heuser M, Blair H, Heidenreich O.
monoclonal anti-
TERT
(
1:500
, #
sc-393013
,
Santa Cruz
),
mouse
monoclonal
anti-Clathrin heavy chain
(
1:1000
, #
ab2731
,
Abcam
),
mouse
monoclonal HRP-conjugated anti-actin (
1:1000
, #
ab49900
,
Abcam
), Rabbit monoclonal anti-GAPDH
(10)
Actin polymerization promotes invagination of flat clathrin-coated lattices in mammalian cells by pushing at lattice edges
Nature Communications
October 17, 2022
Changsong Yang, Patricia Colosi, Siewert Hugelier, Daniel Zabezhinsky, Melike Lakadamyali, Tatyana Svitkina
in PBS at 4 °C. Cells then were incubated with
mouse
monoclonal X-22 clathrin heavy chain antibody (#
Ab2731
,
Abcam
;
1:1000
in 5% BSA in PBS) for 45 min at room temperature. After washing five times with 0.2%
(11)
A method for Boolean analysis of protein interactions at a molecular level
Nature communications
August 13, 2022
Raykova D, Kermpatsou D, Malmqvist T, Harrison PJ, Sander MR, Stiller C, Heldin J, Leino M, Ricardo S, Klemm A,
et al
.
anti-Calreticulin CloneD3E6,
1:100
,"
Cell Signaling
, #
12238
, 5"
Mouse
anti-Clathrin
Clone
X22,
1:500
,"
Abcam
,
ab2731
, GR3412763-1" Rabbit anti-PDGFRβ
Clone
28E1,
1:200
,"
Cell Signaling
, #
3169
, 13"
Mouse
anti-
ACE2
Clone
(12)
Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields
Nature Communications
December 1, 2021
Adrien Mau, Karoline Friedl, Christophe Leterrier, Nicolas Bourg, Sandrine Lévêque-Fort
with intermediary washing steps. α-tubulin (
Sigma Aldrich
,
T6199
) and clathrin heavy-chain (
Abcam
,
ab2731
) primary antibodies were conjugated with Rb-AF647 (
Life Technologies
,
A21237
). Cells were finally postfixed
(13)
Rapid tool for cell nanoarchitecture integrity assessment
Journal of Structural Biology
December 1, 2021
Guido Gaietta, Mark F. Swift, Niels Volkmann, Dorit Hanein
media. The antibodies used in these immunostainings were: (LM
assays
)
mouse
monoclonal anti clathrin (
ab2731
;
Abcam
); rabbit anti giantin (
ab80864
;
Abcam
);
goat
anti
mouse
, Alexa 488 (R37120; Alexa Fluor 488,
(14)
Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes
iScience
November 19, 2021
Kazan JM, Desrochers G, Martin CE, Jeong H, Kharitidi D, Apaja PM, Roldan A, St Denis N, Gingras AC, Lukacs GL,
et al
.
FRIA,
Invitrogen
,
14-0048-82
Flag-Tag (M2),
Sigma
,
F1804
eEF2
,
Cell Signaling
,
2332
a-Tubulin,
Sigma
,
T6074
Clathrin (X22),
Abcam
,
Ab2731
EEA1
,
Invitrogen
,
14-9114-82
anti-
Rat
HRP F(ab')2,
Jackson ImmunoResearch
,
712-036-153
anti-Mouse HRP
(15)
Mouse models for hereditary spastic paraplegia uncover a role of PI4K2A in autophagic lysosome reformation
Autophagy
November 2, 2021
Mukhran Khundadze et al.
rabbit anti-GAPDH,
1:1,000
for WB
(
Santa Cruz Biotechnology
,
sc-25778
);
mouse
anti-clathrin (
Abcam
,
ab2731
)
1:1,000
for IF; rabbit anti-DNM2/Dynamin-2
1:15,000
(R2641, a generous gift of IGBMC). Horseradish
(16)
Green nanotechnology of MGF-AuNPs for immunomodulatory intervention in prostate cancer therapy
Scientific Reports
August 18, 2021
Menka Khoobchandani, Aslam Khan, Kavita K. Katti, Velaphi C. Thipe, Amal Y. Al-Yasiri, Darsha K. D. MohanDoss, Michael B. Nicholl, Ademar B. Lugão, Chetan P. Hans, Kattesh V. Katti
FITC Annexin V
Apoptosis Detection
Kit was obtained from
BD
Pharmingen, USA. X-22 anti-clathrin antibody (
ab2731
), anti-Caveolin-1 antibody (
ab2910
), anti-fibronectin antibody (
ab18265
), and
in vitro
angiogenesis
(17)
Growth cone repulsion to Netrin-1 depends on lipid raft microdomains enriched in UNC5 receptors
Cellular and molecular life sciences : CMLS
March 1, 2021
Hernaiz-Llorens M, Roselló-Busquets C, Durisic N, Filip A, Ulloa F, Martínez-Mármol R, Soriano E.
Abcam
), polyclonal rabbit anti-caveolin-1 (
ab2910
;
Abcam
), monoclonal
mouse
anti-clathrin heavy chain (
ab2731
;
Abcam
), polyclonal rabbit anti-GFP (
A11122
,
Invitrogen
-Thermo Fisher Scientific), wheat germ agglutinin
(18)
Peculiar protrusions along tanycyte processes face diverse neural and nonneural cell types in the hypothalamic parenchyma
The Journal of comparative neurology
February 1, 2021
Pasquettaz R, Kolotuev I, Rohrbach A, Gouelle C, Pellerin L, Langlet F.
western blots
of
Hela Cell
Extracts. The
mouse
monoclonal antibody to CLTC (Clathrin Heavy Chain) (
Abcam
Cat#
ab2731
, RRID:AB_303256) detects clathrin heavy chain and stains the expected band at 190 kDa on
western blots
(19)
A Super-Resolved View of the Alzheimer's Disease-Related Amyloidogenic Pathway in Hippocampal Neurons
Journal of Alzheimer's disease : JAD
January 1, 2021
Yu Y, Gao Y, Winblad B, Tjernberg LO, Schedin-Weiss S.
of Aβ42,NA*,
1:200
/400,In house 22C11,M,APP-NT,3043557,
1:250
,MERCK Clathrin heavy chain,M,Clathrin,
Ab2731
,
1:100
,
Abcam
EEA1
,GP,Early
endosomes
,
237105
,
1:500
,
Synaptic Systems
Rab7,M,
Lysosomes
/LEs,
Ab50533
,
1:500
,
Abcam
Rab9,M,LEs/
lysosomes
,
Ab2810
,
1:200
,
Abcam
Rab3,M,
Synaptic vesicles
(20)
Complimentary action of structured and unstructured domains of epsin supports clathrin-mediated endocytosis at high tension
Communications Biology
December 8, 2020
Jophin G. Joseph, Carlos Osorio, Vivian Yee, Ashutosh Agrawal, Allen P. Liu
solution (in PBS). Primary antibodies against epsin (
Abcam
:
ab75879
(
1:500
dilution)),
CHC
(
Abcam
;
ab2731
(
1:500
dilution)), α-adaptin (
Abcam
:
ab2807
(
1:100
dilution)) were used to quantify the expression levels
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Anti-Clathrin heavy chain antibody [X22] from Abcam
Supplier Page
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Pricing Info
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Company Info
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