Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade ab14984 from Abcam

Product Specs
ItemAnti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab14984
Antibody TypePrimary
ApplicationsChIP, Western blot, ChIP, Western blot
Quantity10 UG, 100 UG
HostMouse, Nude mouse
IsotypeIgG1
ClonalityMonoclonal
Clone3G230 / NM-11
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaEpigenetics
Gene NameEP300
ReactivityHuman
TargetKAT3B / p300

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) HDAC8-mediated inhibition of EP300 drives a transcriptional state that increases melanoma brain metastasisNature CommunicationsNovember 29, 2023Michael F. Emmons, Richard L. Bennett, Alberto Riva, Kanchan Gupta, Larissa Anastasio Da Costa Carvalho, Chao Zhang, Robert Macaulay, Daphne Dupéré-Richér, Bin Fang, Edward Seto, et al.Agarose and a 1/20 aliquot was saved as input control. DNA were immunoprecipitated using an anti-EP300 (ab14984, Abcam) and anti-IgG2a (ab18413, Abcam) antibody overnight at 4 °C. Following a 4-h protein A/G incubation,

(2) METTL3‐mediated N6‐methyladenosine exacerbates ferroptosis via m6A‐IGF2BP2‐dependent mitochondrial metabolic reprogramming in sepsis‐induced acute lung injuryClinical and Translational MedicineSeptember 15, 2023Hao Zhang, Dan Wu, Yanghanzhao Wang, Kefang Guo, Charles B. Spencer, Lilibeth Ortoga, Mengdi Qu, Yuxin Shi, Yuwen Shao, Zhiping Wang, et al.chemiluminescence kit (Tanon). The following primary antibodies were used: anti‐P300 antibody (1:1000, ab14984, Abcam), anti‐METTL3 antibody (1:1000, ab195352, Abcam), anti‐Histone H3 antibody (1:1000, 17168‐1‐AP,

(3) Multiple Fra-1-bound enhancers showing different molecular and functional features can cooperate to repress gene transcriptionCell & BioscienceJuly 18, 2023Fabienne Bejjani, Emilie Evanno, Samantha Mahfoud, Claire Tolza, Kazem Zibara, Marc Piechaczyk, Isabelle Jariel-Encontreand the anti-H3 antibody (05–928) from Millipore. The anti-H3K27ac (Ab4729) and -p300/CBP antibodies (Ab14984) were from Abcam. The anti-rabbit (sc-2313) and anti-mouse (sc-2954) horseradish peroxidase (HRP)-conjugated

(4) Paradoxical Induction of ALOX15/15B by Cortisol in Human Amnion Fibroblasts: Implications for Inflammatory Responses of the Fetal Membranes at ParturitionInternational journal of molecular sciencesJune 29, 2023Zhang F, Lu JW, Lei WJ, Li MD, Pan F, Lin YK, Wang WS, Sun K.optimal size of around 500 bp, which was then immunoprecipitated with antibodies against p300 (Abcam; #ab14984), H3K27ac (Abcam; #ab4729), GR (Cell Signaling; #12041S), total CREB (Cell Signaling; #9104S) or total

(5) A Tumor-suppressive Molecular Axis EP300/circRERE/miR-6837-3p/MAVS Activates Type I IFN Pathway and Antitumor Immunity to Suppress Colorectal CancerClinical cancer research : an official journal of the American Association for Cancer ResearchJune 1, 2023Ding N, You AB, Yang H, Hu GS, Lai CP, Liu W, Ye F.chromatin DNA into 300–500 bp. Supernatants were immunoprecipitated with anti-EP300 antibody (Abcam #ab14984, RRID:AB_301550) or control IgG overnight at 4°C, followed by incubation with protein G magnetic beads

(6) Hypoxia Increases ATX Expression by Histone Crotonylation in a HIF-2α-Dependent MannerInternational journal of molecular sciencesApril 11, 2023Qu M, Long Y, Wang Y, Yin N, Zhang X, Zhang J.against β-actin (sc-8432) were purchased from Santa Cruz (Santa Cruz, CA, USA). Antibodies against p300 (ab14984), H3K9ac (ab4441), H3K18ac (ab1191), and H3K27ac (ab4729) were obtained from Abcam (Cambridge, UK).

(7) Comprehensive 3D epigenomic maps define limbal stem/progenitor cell function and identityNature CommunicationsDecember 1, 2022Mingsen Li et al.CTCF (Millipore, 07-729, 10 μg/ChIP), SMC1 (Bethyl Laboratories, A300-055A, 10 μg/ChIP), EP300 (Abcam, ab14984, 10 μg/ChIP) and H3K9me2 (Abcam, ab1220, 10 μg/ChIP). ChIP-seq data analysis ChIP-Seq data analysis

(8) LINC00472 inhibits cell migration by enhancing intercellular adhesion and regulates H3K27ac level via interacting with P300 in renal clear cell carcinomaCell death discoveryNovember 12, 2022Wang S, Luo C, Li B, Zhang S, Liao W, Xin Q, Xu N, Xie W, Zhu Y, Zhang Y.inhibitor calculator). Then lysed cells were incubated with 5 μg EZH2 (Abcam, ab191250) or P300 (Abcam, ab14984) antibody overnight at 4 °C. Next, using polysome lysis buffer to wash the non-specifically binding

(9) SOX transcription factors direct TCF-independent WNT/β-catenin responsive transcription to govern cell fate in human pluripotent stem cellsCell reportsAugust 23, 2022Mukherjee S, Luedeke DM, McCoy L, Iwafuchi M, Zorn AM.,Bethyl Laboratories,Cat#A300–774A; RRID:AB_669756 NIPBL,Bethyl Laboratories,Cat#A301–779A; RRID:AB_1211232 p300,Abcam,Cat#ab14984; RRID:AB_301550 PYGO2,Novus Biologicals,Cat#NBP1–46171; RRID:AB_10009403 SMC1,Bethyl Laboratories,Cat#A300–055A;

(10) Involvement of activator protein-1 family members in β-catenin and p300 association on the genome of PANC-1 cellsHeliyonFebruary 2, 2022Tomomitsu Doi, Hironori Hojo, Shinsuke Ohba, Kunie Obayashi, Motoyoshi Endo, Toshimasa Ishizaki, Akira Katoh, Hiroyuki Koujiwas immunoprecipitated with anti-β-catenin (Cell signaling, D10A8), CBP (Abcam, ab2832), p300 (Abcam, ab14984), and c-JUN (Abcam, ab31419) antibodies. Libraries were prepared with ThruPLEX DNA-seq Kit (Takara-bio,

(11) Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagyActa pharmaceutica Sinica. BFebruary 1, 2022Sun X, Shu Y, Ye G, Wu C, Xu M, Gao R, Huang D, Zhang J.12789-1-AP), COXⅣ (CST, 11967), cleaved-PARP-1 (CST, 5625), cleaved-caspase3 (CST, 9661), EP300 (Abcam, ab14984), FLAG (Sangon Biotech, D191041), anti-FLAG® M2 Affinity Gel (MERCK, F2426), GAPDH (Proteintech, 600004-1-lg),

(12) P-gp expression inhibition mediates placental glucocorticoid barrier opening and fetal weight lossBMC MedicineDecember 8, 2021Caiyun Ge, Dan Xu, Pengxia Yu, Man Fang, Juanjuan Guo, Dan Xu, Yuan Qiao, Sijia Chen, Yuanzhen Zhang, Yuanzhen ZhangCo., Ltd. (Beijing, China). Primary antibodies anti-P-gp (ab170904), anti-YB-1 (ab76149), anti-P300 (ab14984), anti-acetyl H3K9 (ab10812), anti-acetyl H3K14 (ab52946), and IgG (ab172730) antibody were purchased

(13) Fms-like tyrosine kinase 3-internal tandem duplications epigenetically activates checkpoint kinase 1 in acute myeloid leukemia cellsScientific ReportsDecember 1, 2021Yudong Zhang, Lingli Yuansize. The fragments were incubated with specific antibodies (anti-CBP (ab 119,488, Abcam), anti-p300 (ab14984, Abcam), anti-H3K9ac (ab272150, Abcam), anti-H3K27ac (ab203953, Abcam) at 4 degrees overnight. Rabbit

(14) Acute depletion of the ARID1A subunit of SWI/SNF complexes reveals distinct pathways for activation and repression of transcriptionCell ReportsNovember 2, 2021Seraina Blümli et al.monoclonal anti-EP300,Santa Cruz Biotechnology,sc-48343; RRID:AB_62807 Mouse monoclonal anti-EP300,Abcam,ab14984; RRID:AB_301550 Rabbit polyclonal anti-H3K27ac,Abcam,ab4729; RRID:AB_2118291 Rabbit monoclonal anti-H3K27me3,Cell Signaling Technology

(15) Enhancer recruitment of transcription repressors RUNX1 and TLE3 by mis-expressed FOXC1 blocks differentiation in acute myeloid leukemiaCell reportsSeptember 21, 2021Simeoni F, Romero-Camarero I, Camera F, Amaral FMR, Sinclair OJ, Papachristou EK, Spencer GJ, Lie-A-Ling M, Lacaud G, Wiseman DH, et al.RRID:AB_2118291 anti-HDAC1,Abcam,Cat#ab46985; RRID:AB_880347 anti-RUNX1,Abcam,Cat#ab23980; RRID:AB_2184205 anti-EP300,Abcam,Cat#ab14984; RRID:AB_301550 anti-TLE3,Abcam,Cat#ab94972; RRID:AB_10860535 anti-CBFb,Abcam,Cat#ab125191; RRID:AB_10999861 anti-MYB,Abcam,Cat#ab117635;

(16) Pyruvate dehydrogenase kinase 1 and 2 deficiency reduces high-fat diet-induced hypertrophic obesity and inhibits the differentiation of preadipocytes into mature adipocytesExperimental & molecular medicineSeptember 1, 2021Kang HJ, Min BK, Choi WI, Jeon JH, Kim DW, Park S, Lee YK, Kim HJ, Byeon JE, Go Y, et al.anti-H4ac (06–598, Millipore), anti-H3K4me3 (07–473, Millipore), anti-PCAF (ab12188, Abcam), anti-p300 (ab14984, Abcam), anti-H3K27ac (ab177178, Abcam) and control mouse IgG (I5381, Sigma-Aldrich)) overnight at 4

(17) PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3Cell death & diseaseJuly 21, 2021Chen D, Zhao Z, Chen L, Li Q, Zou J, Liu S.(#9402, Cell Signaling Technology), MED1 (#17-10530, EMD Millipore, Temecula, CA, USA), and EP300 (#ab14984, Abcam) were used. The primers against the promoter regions and distal desert regions were listed as

(18) Epigenetic regulation of intestinal peptide transporter PEPT1 as a potential strategy for colorectal cancer sensitizationCell Death & DiseaseJune 1, 2021Yanhong Wang, Jiaqi Wang, Lingrong Yang, Liqing Qiu, Yuhui Hua, Shixiu Wu, Su Zeng, Lushan Yu, Xiaoli Zheng(1:1000, Abcam Cat# ab13537), anti-HDAC1 (1:2000, Abcam Cat# ab7028), anti-KAT3B/p300 (1;1000, Abcam Cat# ab14984), and anti-GAPDH (1:3000, Multi Sciences, Cat# Mab5465)] were incubated with PVDF membranes overnight

(19) CDX2 enhances natural killer cell–mediated immunotherapy against head and neck squamous cell carcinoma through up‐regulating CXCL14Journal of Cellular and Molecular MedicineMay 1, 2021Haitao Wang, Shanji Nan, Ying Wang, Chengbi XuHEPES, pH 7.9, 0.2 mM EDTA, 0.5 mM DTT, 20% glycerol, 0.2% NP‐40 and 200 mM KCl). Then, 5 µg p300 (ab14984; Abcam) or IgG control (ab157532; Abcam) was incubated with Dynabeads for at least 6 hours. 200 µg portions

(20) FGF23 ameliorates ischemia-reperfusion induced acute kidney injury via modulation of endothelial progenitor cells: targeting SDF-1/CXCR4 signalingCell death & diseaseApril 17, 2021Chang HM, Peng KY, Chan CK, Sun CY, Chen YY, Chang HM, Huang CL, Liu PC, Chen PY, Wang KC, et al.(Santa Cruz, sc-8414X, 1:500), GLP (Abcam, ab41969, 1:250), H3K9me2 (Abcam, ab1220, 1:250), p300 (Abcam, ab14984, 1:250) or control (Cell Signaling, #2729, 1:500) antibodies. PCR analyses were carried out for 36 cycles

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Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade from Abcam

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Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade from Abcam

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