Fig 1: 6b attenuates HG-induced myocardial oxidative stress in H9c2 cells. H9c2 cells were pre-treated with indicated dose of 6b for 1 hr and then incubated with HG (33 mM) for 12 hrs. The cell lysates were immunoblotted for Nrf2, HO-1 and NQO-1, respectively (A). The mRNA expression levels of Nrf2 (B), NQO-1 (C) and HO-1 (D) were determined. Cells were subjected to immunofluorescence assay as described in Materials and methods. Images were acquired (E) and quantified (F). Normalized values are plotted. *P < 0.05, **P < 0.01 versus CON; ## P < 0.01, ### P < 0.001 versus HG.