Fig 1: Validation of anti–chemokine (C‐X‐C motif) receptor 4 (CXCR4) antibodies. Human vascular smooth muscle cells were incubated with nontargeting (NT) or CXCR4 small interfering RNA (siRNA) and then used in proximity ligation assays (PLAs) for the detection of CXCR4 with goat anti‐CXCR4 (Abcam Ab1670) and rabbit anti‐CXCR4 (Alomone Labs ACR‐014). A, Representative PLA images showing merged PLA/4′,6‐diamidino‐2‐phenylindole dihydrochloride signals. Ctrl: Omission of primary antibody. Scale bars=10 μm. B, Quantification of PLA signals per cell, as in (A). n=10 images per condition. *: p<0.05 vs. cells incubated with NT siRNA.
Supplier Page from Abcam for Anti-CXCR4 antibody