Fig 2: Altered mRNA expression of genes, encoding Z-disk proteins, in different parts of myocardium. (A) Actn2, Cmya5, and Nebl expression patterns after different period of aortic banding measured by qRT-PCR – downregulation after 1 week with subsequent return to normal level and further decrease after 10 weeks. Expression Ldb3 declined after 1, 2, and 10 weeks of aortic banding. (B) Altered expression of genes, encoding Z-disk proteins, in right ventricle (RV). Actn2, Cmya5, and Ldb3 expression was gradually increasing after 2 weeks up to 10 weeks of aortic constriction. Despite the gradual decrease of Nebl expression in RV, we showed no further Nebl reduction to the 10 week’s time point. Notably, expression pattern in RV is clearly distinct from pattern in LV. (C) Expression pattern of genes, encoding Z-disk proteins, in IVS are distinctly more close to RV than to LV profiles. Cmya and Nebl mRNA levels were elevated on later phases of hypertrophy progression (after 10 weeks). Opposite, Actn2 is upregulated only on the early phase (after 1 week). Ldb3 mRNA level was significantly increased after 1 week as well as after 10 weeks of aortic constriction. For all *P < 0.05.