Fig 1: Correlation between Vav1 and Akt isoforms in MDA-MB-231 cells. (A) Representative immunochemical analysis of lysates from MDA-MB-231 cells transfected with siRNAs specific for Vav1 (Vav1 siRNAs) or with a construct expressing Vav1 (Over Vav1). Scramble siRNAs (Ctrl siRNAs) and an empty vector were used as controls. ß-Tubulin was blotted as an internal control of loaded proteins. (B) qRT-PCR analysis of mRNA (2-?Ct method) and (C) representative Western blot of Akt1, Akt2 and Akt3 in MDA-MB-231 cells cultured under the above reported experimental conditions. (D) Levels of Akt isoforms as deduced from the chemiluminescence signal normalized with ß-Tubulin. Asterisks indicate statistically significant differences with respect to corresponding controls taken as 1. All the data are the means of 3 separate experiments ± SD. * p < 0.05.