Anti-TLR4 antibody ab13867 from Abcam

Product Specs
ItemAnti-TLR4 antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab13867
Antibody TypePrimary
ClonalityPolyclonal
Quantity100 UG
IsotypeIgG
HostRabbit
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
Concentration0.5 mg/ml
Research AreaImmuno-oncology
Gene NameTlr4
ReactivityHuman, Mouse
TargetTLR4

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Jianpi Yangxue Qufeng compound alleviates atopic dermatitis via TLR4/MyD88/NF-κB signaling pathwayHeliyonDecember 3, 2023Xuesong Yang, Zhimin Wang, Hong Huang, Guangyun Luo, Lin Cong, Jianting Yang, Jianzhou YeAmersham Imager 600. The primary antibodies used were as follows: GAPDH (6004-1, Proteintech), TLR4 (ab13867, Abcam), MyD88 (#4283, Cell Signaling Technology), NF-κB (#8242, Cell Signaling Technology), and Histone

(2) Rutin alleviates ventilator-induced lung injury by inhibiting NLRP3 inflammasome activationiScienceOctober 20, 2023Shengsong Chen, Yu Bai, Jingen Xia, Yi Zhang, Qingyuan ZhanUK), NLRP3 (Abcam, ab214185, UK), ASC (CST, 67824, USA), Caspase1 (Abcam, ab138483, UK), TLR4 (Abcam, ab13867, UK), MD2 (Abcam, ab24182, UK), p-P65 (Thermo, MA5-15160, USA) and P65 (Abcam, ab16502, UK) at 4°C overnight.

(3) Herbal melanin modulates PGE2 and IL-6 gastroprotective markers through COX-2 and TLR4 signaling in the gastric cancer cell line AGSBMC Complementary Medicine and TherapiesSeptember 1, 2023Adila El-Obeid, Yahya Maashi, Rehab AlRoshody, Ghada Alatar, Modhi Aljudayi, Hamad Al-Eidi, Nouf AlGaith, Altaf Husain Khan, Adil Hassib, Sabine Matou-Nasri(#TA328217, OriGene Technologies Inc., Rockville, MD, USA), rabbit monoclonal anti-TLR4 antibody (#ab13867, Abcam, Cambridge, UK), rabbit monoclonal phospho-p65 (Ser 529) NF-κB (#44711G, Invitrogen, Thermo Fischer

(4) Tuo-Min-Ding-Chuan Decoction Alleviates Airway Inflammations in the Allergic Asthmatic Mice Model by Regulating TLR4-NLRP3 Pathway-Mediated Pyroptosis: A Network Pharmacology and Experimental Verification StudyDrug Design, Development and TherapyJune 2, 2023Mingsheng Lyu, Jingbo Qin, Shuaiyang Huang, Dongmei Shao, Guirui Huang, Fan Yang, Xuefeng Gong, Shiyu Zhang, Zhijie Zhang, Ji Wang, et al.radio-immunoprecipitation assay (RIPA) lysis buffer [Beijing Applygen, C1053], anti-TLR4 antibody [Abcam, ab13867], anti-TRAF6 antibody [Abcam, ab33915], anti-NLRP3 antibody [Abcam, ab263899], anti-Caspase-1 antibody

(5) Mild treadmill exercise inhibits cartilage degeneration via macrophages in an osteoarthritis mouse modelOsteoarthritis and cartilage openJune 1, 2023Oka Y, Murata K, Ozone K, Minegishi Y, Kano T, Shimada N, Kanemura N.Cell Signaling Technology, Inc., MA, USA, 1:200), anti-CD206 (18704-1-AP; Proteintech Group, IL, USA, 1:500), anti-TLR4 (ab13867; Abcam plc. MA, USA, 1:250), and anti-IL-4 (GTX66741; Gene Tex Inc., CA, USA, 1:100) were used as primary

(6) Toll-like receptor 4 signaling in osteoblasts is required for load-induced bone formation in miceiScienceApril 21, 2023Ibtesam Rajpar, Gaurav Kumar, Paolo Fortina, Ryan E. Tomlinsonwith normal goat serum, before overnight incubation with a rabbit, anti-mouse TLR4 antibody (Abcam ab13867) at 4 °C. The next day, sections were washed and treated with a goat anti-rabbit IgG fluorescent secondary

(7) Tectorigenin alleviates the apoptosis and inflammation in spinal cord injury cell model through inhibiting insulin-like growth factor-binding protein 6Open Med (Wars)April 11, 2023Liqiang Zhou, Kui Yan, Shuxing Xing, Jun ChengCell signaling technology), caspase-9 (#9504, rabbit antibody, 49 kDa, dilution 1:1000, Cell signaling technology), TLR4 (ab13867, rabbit polyclonal, 90 kDa, dilution 1:500; Abcam, Cambridge, UK), p-IκBα (# 2859, rabbit monoclonal,

(8) Complement C3 mediates podocyte injury through TLR4/NFΚB-P65 signaling during ischemia–reperfusion acute kidney injury and post-injury fibrosisEuropean Journal of Medical ResearchMarch 27, 2023Yi Chen, Liyu Lin, Siyi Rao, Xuan Tao, Jiong Cui, Jianxin Wanpotential channel 6 (TRPC6) (Cat.ab233413, Abcam, 1:100), C3 (Cat.ab200999, Abcam, 1:200), TLR4 (Cat.ab13867, Abcam, 1:200), and NFκB-P65 (Cat.3033, CST, 1:200), at 37℃ for 1 h, and rinse with phosphate-buffered

(9) Extracellular vesicles derived from CD4(+) T cells carry DGKK to promote sepsis-induced lung injury by regulating oxidative stress and inflammationCellular & molecular biology lettersMarch 23, 2023Tu GW, Zhang Y, Ma JF, Hou JY, Hao GW, Su Y, Luo JC, Sheng L, Luo Z.non-fat milk. Next, it was added with primary antibodies against DGKK (Abcam; ab103681), TLR4 (Abcam; ab13867), NOX4 (Abcam; ab154244), NF-κBp65 (Cell Signaling Technology, Danvers, MA, USA; #8242), H3 (Cell Signaling Technology

(10) Gypenosides counteract hepatic steatosis and intestinal barrier injury in rats with metabolic associated fatty liver disease by modulating the adenosine monophosphate activated protein kinase and Toll-like receptor 4/nuclear factor kappa B pathwaysPharmaceutical BiologyDecember 31, 2022Shuhua Shen, Kungen Wang, Yihui Zhi, Yue Dongelement-binding protein 1c (SREBP-1c, ab28481, 68 kDa, 1:500, Abcam, Cambridge, MA), Toll-like receptor 4 (TLR4; ab13867, 90 kDa, 1:1000, Abcam, Cambridge, MA), phosphorylated (p)-p65 (ab76302, 65 kDa, 1:1000, Abcam, Cambridge,

(11) Spinal NLRP3 inflammasome activation mediates IL-1β release and contributes to remifentanil-induced postoperative hyperalgesia by regulating NMDA receptor NR1 subunit phosphorylation and GLT-1 expression in ratsMolecular PainNovember 24, 2022Yuan Yuan, Yue Zhao, Mengxi Shen, Chenxu Wang, Beibei Dong, Keliang Xie, Yang Yu, Yonghao YuAbcam), rabbit anti-GLT-1 (cat. no, ab106289; dilution, 1:1,000; Abcam), rabbit anti-TLR4 (cat. no, ab13867; dilution, 1:1,200; Abcam), rabbit anti-P2X7R (cat. no, ab48871; dilution, 1:1,000; Abcam), rabbit anti-NLRP3

(12) Osteocytes directly regulate osteolysis via MYD88 signaling in bacterial bone infectionNature communicationsNovember 4, 2022Yoshimoto T, Kittaka M, Doan AAP, Urata R, Prideaux M, Rojas RE, Harding CV, Henry Boom W, Bonewald LF, Greenfield EM, et al.were incubated with antibodies against MYD88 (LS-C357983, LSBio), TLR2 (NB100-56720, Novus), TLR4 (ab13867, Abcam), F4/80 (Clone A3-1, Bio-Rad), Ly-6G (sc-53515, Santa Cruz Biotechnology) or Pg (DMAB9447, Creative Diagnostics

(13) Timeâ€‘dependent changes in NLRP3 and Nrf2 levels in lipopolysaccharideâ€‘induced acute lung injuryInternational Journal of Molecular MedicineOctober 27, 2022Rana Dhar, Ning Li, Lejun Zhang, Yajun Li, Mohammad Nasiruddin Rana, Xinwei Cao, Zhengqiang Hu, Xuefeng Wang, Xuyang Zheng, Xuanli Xu, et al.BSA. The membranes were then incubated with the following primary antibodies: TLR-4 (1:1,000; cat. no. ab13867, Abcam), anti-NLRP3 (1:1,000; cat. no. AG-20B-0014, Adipogen Life Sciences); anti-procaspase-1/cleaved

(14) Activation of Toll-like receptor 4 by Ebola virus-shed glycoprotein is direct and requires the internal fusion loop but not glycosylationCell ReportsOctober 25, 2022Michael J. Scherm, Monique Gangloff, Nicholas J. Gay(ab238343, Abcam) and TLR4 (ab233665, Abcam) were incubated with 2 μL anti-MD2 (ab24182, Abcam), anti-TLR4 (ab13867, Abcam) and anti-His (51-9000012, BD-Pharmagen) antibodies at 4°C for 4 h followed by addition of 20

(15) Adiponectin reduces apoptosis of diabetic cardiomyocytes by regulating miR-711/TLR4 axisDiabetology & Metabolic SyndromeSeptember 16, 2022Yu Zuo, Tao Xiao, Xiangdong Qiu, Zuoliang Liu, Shengnan Zhang, Na Zhouin 5% skimmed milk at room temperature for 1 h and incubated with antibodies against TLR4 (1:1000, ab13867, Abcam, USA), phosphorylated (p)-NF-κB p65 (1:1000, ab76302, Abcam), Bax (1:1000, ab32503, Abcam), Bcl-2

(16) IL35 attenuated LPS-induced acute lung injury by regulating macrophage polarizationMolecular Biology ReportsJuly 1, 2022Shengsong Chen, Jingen Xia, Yi Zhang, Qingyuan Zhanp35 (R&D Systems, MAB6688, USA), Bax (Abcam, ab32503, USA), Bcl2 (Abcam, ab182858, USA), TLR4 (Abcam, ab13867, USA), MD2 (Abcam, ab24182, USA), p-P65 (Abclonal, AP0475, China) or P65 (Abcam, ab16502, USA) at 4

(17) Long noncoding RNA X-inactive specific transcript regulates NLR family pyrin domain containing 3/caspase-1-mediated pyroptosis in diabetic nephropathyWorld journal of diabetesApril 15, 2022Xu J, Wang Q, Song YF, Xu XH, Zhu H, Chen PD, Ren YP.(1:100, sc-514414, Santa Cruz Biotechnology, TX, United States), TLR4 rabbit polyclonal antibody (1:500, ab13867, Abcam, Cambridge, United Kingdom), and GAPDH (1:2500, ab9485, Abcam, Cambridge, United Kingdom). Immunofluorescence The

(18) Human umbilical cord mesenchymal stem cells contribute to the reconstruction of bladder function after acute spinal cord injury via p38 mitogen-activated protein kinase/nuclear factor-kappa B pathwayBioengineeredMarch 1, 2022Jue Li, Jiliang Huang, Ling Chen, Wei Ren, Wenzhi Caireceptor potential vallinoid 1 (TRPV1) (ab203103, Abcam, UK, 95 kDa, 1:1000), toll-like receptor 4 (TLR4) (ab13867, Abcam, UK, 90 kDa, 1:500), NF-κB (#8242, cell signaling technology (CST), USA, 65 kDa, 1:1000), p-NF-κB

(19) Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in MiceInternational journal of molecular sciencesFebruary 28, 2022Chang ZY, Liu HM, Leu YL, Hsu CH, Lee TY.(ab15098, Abcam, Trumpington, Cambridge, UK), occludin (ab216327, Abcam, Trumpington, Cambridge, UK), TLR4 (ab13867, Abcam, Trumpington, Cambridge, UK), MyD88 (ab28763, Abcam, Trumpington, Cambridge, UK), p-p38 (ab178867,

(20) HOXA11-AS aggravates microglia-induced neuroinflammation after traumatic brain injuryNeural Regeneration ResearchJanuary 1, 2022Xiang-Long Chen, Jian Wang, Xiang-Long Yang, Li-Gang Wang, Fu-Bing Yanganti-aquaporin-4 (AQP4) (1:1000, Cat# ab46182, RRID:AB_955676, Abcam), rabbit anti-TLR4 (1:1000, Cat# ab13867, RRID:AB_300696, Abcam), rabbit anti-phosphorylation-NF-κB p65 (p-NF-κB p65, 1:1000, Cat# ab97726, RRID:AB_10681170,)

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Anti-TLR4 antibody from Abcam

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Anti-TLR4 antibody from Abcam

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