Anti-ATPB antibody [3D5] - Mitochondrial Marker ab14730 from Abcam

Product Specs
ItemAnti-ATPB antibody [3D5] - Mitochondrial Marker
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab14730
Antibody TypePrimary
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Clone3D5
Quantity100 UG
IsotypeIgG1
ClonalityMonoclonal
HostMouse, Nude mouse
Conjugate/TagUnconjugated
FormatNear homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation
Concentration1 mg/ml
Gene NameATP5F1B
ReactivityCaenorhabditis elegans, Caenorhabditis elegans, Cow, Human, Mouse, Rat
TargetATPB

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Astrocyte and L-lactate in the anterior cingulate cortex modulate schema memory and neuronal mitochondrial biogenesiseLifeNovember 14, 2023Mastura Akter, Mahadi Hasan, Aruna Surendran Ramkrishnan, Zafar Iqbal, Xianlin Zheng, Zhongqi Fu, Zhuogui Lei, Anwarul Karim, Ying LiPolyclonal),Abcam,Cat #: ab191838,1: 500 (IHC) Antibody,Anti-ATPB (Mouse Monoclonal),Abcam,Cat #: ab14730,1: 500 (IHC) Antibody,Anti-MCT2 (Rabbit Polyclonal),Merck Millipore,Cat #: AB3542,1: 500 (WB) Antibody,Anti-β-actin

(2) NAD+ regulates nucleotide metabolism and genomic DNA replicationNature Cell BiologyNovember 13, 2023Sebastian Howen Nesgaard Munk, Joanna Maria Merchut-Maya, Alba Adelantado Rubio, Arnaldur Hall, George Pappas, Giacomo Milletti, MyungHee Lee, Lea Giørtz Johnsen, Per Guldberg, Jiri Bartek, et al.(Cell Signaling 4661, 1:500), CIV (Cell Signaling 11967, 1:500), OPA1 (Abcam ab157457, 1:1,000) and ATPB (Abcam ab14730, 1:50). Total NAD(H) quantification Total NAD(H) was extracted and measured using the colourimetric

(3) Mitochondrial defects caused by PARL deficiency lead to arrested spermatogenesis and ferroptosiseLifeJuly 28, 2023Enrico Radaelli, Charles-Antoine Assenmacher, Jillian Verrelle, Esha Banerjee, Florence Manero, Salim Khiati, Anais Girona, Guillermo Lopez-Lluch, Placido Navas, Marco Spinazzi,BD Biosciences,Cat#: 611562;RRID:AB_399008,"WB 1:50,000" Antibody,Anti-ATP5B (mouse monoclonal),Abcam,Cat#: ab14730;RRID:AB_301438,"WB 1:50,000" Antibody,Anti-TOMM20 (rabbit polyclonal),Santa Cruz,Cat#: sc-11415;RRID:AB_2207533,WB

(4) The Impact of Resveratrol-Enriched Bread on Cardiac Remodeling in a Preclinical Model of DiabetesAntioxidants (Basel)May 9, 2023Andreia F. R. Silva, Rita Silva-Reis, Rita Ferreira, Paula A. Oliveira, Ana I. Faustino-Rocha, Maria de Lurdes Pinto, Manuel A. Coimbra, Artur M. S. Silva, Susana M. Cardosowere incubated with primary antibody to detect ATP synthase subunit beta (mouse anti-ATPβ, 1:1000, ab14730, Abcam), phosphofructokinase, muscle isoform (rabbit anti-PFKM, 1:1000, ab154804, Abcam), CAAT enhancer-binding

(5) TSPO acts as an immune resistance gene involved in the T cell mediated immune control of glioblastomaActa neuropathologica communicationsMay 8, 2023Menevse AN, Ammer LM, Vollmann-Zwerenz A, Kupczyk M, Lorenz J, Weidner L, Hussein A, Sax J, Mühlbauer J, Heuschneider N, et al.4 °C with rabbit anti-TSPO antibody (Abcam, #ab10949, 1:500) and mouse anti-ATPB antibody (Abcam, #ab14730, 1:1000) in 0.1% Triton X-100, 2% donkey serum in 1x PBS. After three washing steps with 1x PBS, cells

(6) Mitochondrial control of microglial phagocytosis by the translocator protein and hexokinase 2 in Alzheimer’s diseaseProceedings of the National Academy of SciencesFebruary 21, 2023Lauren H. Fairley, Kei Onn Lai, Jia Hui Wong, Wei Jing Chong, Anselm Salvatore Vincent, Giuseppe D’Agostino, Xiaoting Wu, Roshan R. Naik, Anusha Jayaraman, Sarah R. Langley, et al.antibodies used were rabbit anti-hexokinase (1:500, Abcam, ab227198), mouse anti-ATPB (1:1,000, Abcam, ab14730), rabbit anti-IBA-1 (1:500, Wako, 019-19741), rabbit anti-GFAP (Abcam, ab7260), Alexa Fluor 488 conjugated

(7) C/EBPβ Regulates TFAM Expression, Mitochondrial Function and Autophagy in Cellular Models of Parkinson’s DiseaseInternational Journal of Molecular SciencesJanuary 11, 2023Ana Sierra-Magro, Fernando Bartolome, David Lozano-Muñoz, Jesús Alarcón-Gil, Elena Gine, Marina Sanz-SanCristobal, Sandra Alonso-Gil, Marta Cortes-Canteli, Eva Carro, Ana Pérez-Castillo, et al.(Santa Cruz, ref. sc166965), mouse anti-LC3 (Santa Cruz, ref. sc376404) mouse anti-ATPB (Abcam, ref. ab14730), rabbit anti-p62 (Sigma, ref. P00067), mouse anti-α-tubulin (Sigma, ref. T9026), and mouse anti-α-actin

(8) Sci-Seq of Human Fetal Salivary Tissue Introduces Human Transcriptional Paradigms and a Novel Cell PopulationFrontiers in Dental MedicineDecember 21, 2022Devon Duron Ehnes, Ammar Alghadeer, Sesha Hanson-Drury, Yan Ting Zhao, Gwen Tilmes, Julie Mathieu, Hannele Ruohola-Baker,R&D Systems(IC1420R-100UG)) Krt15,KRT15,Rabbit,1:1000,Sigma(HPA023910–25UL) Atpb,ATPB,Mouse,1:100,Abcam(ab14730) Nkccl,SLC12A2,Rabbit,1:100,Cell Signaling Technologies(8351S) Ezh2,EZH2,Rabbit,1:100,Cell Signaling Technologies

(9) DJ-1 is an essential downstream mediator in PINK1/parkin-dependent mitophagyBrain : a journal of neurologyDecember 19, 2022Imberechts D, Kinnart I, Wauters F, Terbeek J, Manders L, Wierda K, Eggermont K, Madeiro RF, Sue C, Verfaillie C, et al.goat anti-HSP60 (IF, 1/500; MyBioSource, MBS423631), mouse anti-ATP5F1B (WB, 1:1000, IF, 1:500; Abcam, ab14730), mouse antimitofusin 2 (WB, 1:1000; Abcam, ab56889), mouse anti-LC3B (WB, 1:2000; Novus Biologicals,

(10) BNIP3L/NIX regulates both mitophagy and pexophagyThe EMBO journalDecember 15, 2022Wilhelm LP, Zapata-Muñoz J, Villarejo-Zori B, Pellegrin S, Freire CM, Toye AM, Boya P, Ganley IG.(Santa Cruz sc‐20011, 1:500 for IF), anti‐GAPDH (Proteintech 60004‐1‐Ig, 1:10,000 for WB), anti‐ATPB (Abcam ab14730, 1:500 for IF), anti‐Ubiquitin (FK2) (Enzo life sciences BML‐PW8810‐0, 1:200 for IF and 1:1,000 for WB

(11) The mitochondrial chaperone TRAP1 regulates F-ATP synthase channel formationCell death and differentiationDecember 1, 2022Cannino G, Urbani A, Gaspari M, Varano M, Negro A, Filippi A, Ciscato F, Masgras I, Gerle C, Tibaldi E, et al.antibody cocktail (cat. #: ab110411) recognizing CI (NDUFB8), CIV (COXII) and CV (α), anti-β (cat. #: ab14730), anti-b (cat. #: ab117991), and anti-OSCP (cat. #: ab110276) mouse monoclonal antibodies, and anti

(12) HSF1 is a driver of leukemia stem cell self-renewal in acute myeloid leukemiaNature communicationsOctober 16, 2022Dong Q, Xiu Y, Wang Y, Hodgson C, Borcherding N, Jordan C, Buchanan J, Taylor E, Wagner B, Leidinger M, et al.(ab189945,1:800 dilution), UQCRC2 (ab14745,1:800 dilution), MTCO1 (ab14705,1:800 dilution), ATP5B (ab14730,1:800 dilution) (all from Abcam), DDK/FLAG (Origene, TA180144, 1:1000 dilution) or beta-actin (Santa Cruz

(13) Generation and characterization of human U-2 OS cell lines with the CRISPR/Cas9-edited protoporphyrinogen oxidase IX geneScientific reportsOctober 12, 2022Novakova Z, Milosevic M, Kutil Z, Ondrakova M, Havlinova B, Kasparek P, Sandoval-Acuña C, Korandova Z, Truksa J, Vrbacky M, et al.anti-MT-CO1 Ab (1 µg/ml; ab14705; Abcam, Cambridge, UK), mouse monoclonal anti-ATP5F1B Ab (0.5 µg/ml; ab14730; Abcam, Cambridge, UK), donkey anti-rabbit secondary Ab conjugated to AlexaFluor-568 (0.3 µg/ml; A10042;

(14) Hyperhomocysteinemia exacerbates acute kidney injury via increased mitochondrial damageFrontiers in PhysiologyOctober 5, 2022Mei Zhang, Rong Dong, Jingjing Da, Jing Yuan, Yan Zha, Yanjun LongMembranes were subsequently probed overnight at 4°C with primary antibodies against ATP β (1:2000, Abcam, ab14730), caspase-3 (1:2000, Abcam, ab184787), caspase-12 (1:2000, Abcam, ab62463), cleaved caspase-3 (1:1000,

(15) Metabolic Adaptation as Potential Target in Papillary Renal Cell Carcinomas Based on Their In Situ Metabolic CharacteristicsInternational Journal of Molecular SciencesSeptember 13, 2022Ildikó Krencz et al.Mitotracker CMXRos (1:10000, #M7512, Invitrogen, Waltham, MA, USA) or immunolabelling by anti-ATPB (1:100, #ab14730; Abcam), anti-COX IV (1:250, #4850, CST), anti-TOM20 (1:500, #42406, CST) primary antibodies, subsequent

(16) Co-transport of the nuclear-encoded Cox7c mRNA with mitochondria along axons occurs through a coding-region-dependent mechanismJournal of cell scienceAugust 15, 2022Cohen B, Altman T, Golani-Armon A, Savulescu AF, Ibraheem A, Mhlanga MM, Perlson E, Arava YS.overnight at 4°C with primary antibodies for ERK1/2 (1:10,000, Sigma Aldrich M5670) and ATPB (1:1000, Abcam ab14730). The membranes were next incubated with secondary anti-HRP antibody (1:10,000, Jackson Laboratories

(17) Mitochondrial matrix-localized Src kinase regulates mitochondrial morphologyCellular and molecular life sciences : CMLSMay 30, 2022Lurette O, Guedouari H, Morris JL, Martín-Jiménez R, Robichaud JP, Hamel-Côté G, Khan M, Dauphinee N, Pichaud N, Prudent J, et al.(#2108S, Cell Signaling), NDUFA9 (ab14713, Abcam), UQCRC2 (ab14742, Abcam), COXIV (ab16056, Abcam), ATPB (ab14730, Abcam), VDAC (ab14734, Abcam), TOM20 (sc-17764, Santa Cruz), SOD2 (#13194S, Cell Signaling), cytochrome

(18) DGAT1 activity synchronises with mitophagy to protect cells from metabolic rewiring by iron depletionThe EMBO JournalMay 16, 2022Maeve Long et al.For immunocytochemistry, the following primary antibodies were used: anti‐ATPB [clone 3D5] (Abcam, Cat# ab14730; Mouse mAb, 1:500; RRID:AB_301438); anti‐BNIP3L/Nix [clone D4R4B] (Cell Signaling Technology, Cat# 12396,

(19) Targeting Endogenous K-RAS for Degradation Through the Affinity-Directed Protein Missile SystemSSRN Electronic JournalApril 3, 2022Sascha Röth, Thomas J. Macartney, Agnieszka Konopacka, Markus A. Queisser, Gopal SapkotaAnti-HIF-1 alpha antibody [H1alpha67] Abcam Cat# Ab1; RRID: AB_296474 Anti-ATPB antibody [3D5] Abcam Cat# Ab14730; RRID: AB_301438 Monoclonal Anti-KRAS antibody SigmaAldrich Cat# WH0003845M1; RRID: AB_1842235 Monoclonal

(20) Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activationMolecular cancerApril 2, 2022Wang Z, Sun A, Yan A, Yao J, Huang H, Gao Z, Han T, Gu J, Li N, Wu H, et al.(ab140519, 1:2000, Abcam, Cambridge, MA, USA), TOX3 (ab216614, 1:2000, Abcam, Cambridge, MA, USA), ATP5B (ab14730, 1:2000, Abcam, Cambridge, MA, USA), APC (2504, 1:1000, CST, Danvers, MA, USA), CK1 (2655, 1:1000, CST,

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Anti-ATPB antibody [3D5] - Mitochondrial Marker from Abcam

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Anti-ATPB antibody [3D5] - Mitochondrial Marker from Abcam

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