Anti-COX IV antibody [20E8C12] ab14744 from Abcam

Product Specs
ItemAnti-COX IV antibody [20E8C12]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab14744
Antibody TypePrimary
ClonalityMonoclonal
Quantity100 UG
IsotypeIgG2a
HostMouse, Nude mouse
Clone20E8C12
ApplicationsFlow Cytometry, Western blot
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameCOX4I1
ReactivityCow, Human, Mouse, Rat
TargetCOX IV

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Tissue-specific differences in the assembly of mitochondrial Complex I are revealed by a novel ENU mutation in ECSITCardiovascular ResearchOctober 16, 2023Thomas Nicol, Sara Falcone, Andrew Blease, Pratik Vikhe, Gabriele Civiletto, Saleh Salman Omairi, Carlo Viscomi, Ketan Patel, Paul K Potter(FACS),N/A CLPP,Rabbit poly,"Abcam, Cambridge, UK",ab124822,1:5000,26 COXIV,Mouse mono,"Abcam, Cambridge, UK",ab14744,1:1000,17 ECSIT,Rabbit poly,"Abcam, Cambridge, UK",ab21288,1:1000,50/45 F4/80—PE,Rat mono,"Thermo Fisher

(2) Focal segmental glomerulosclerosis with a mutation in the mitochondrially encoded NADH dehydrogenase 5 gene: A case reportMolecular Genetics and Metabolism ReportsJune 1, 2023Tsukasa Naganuma, Toshiyuki Imasawa, Ikuo Nukui, Masakiyo Wakasugi, Hiroshi Kitamura, Yukiko Yatsuka, Yoshihito Kishita, Yasushi Okazaki, Kei Murayama, Yoshimi Jingujiwith anti-cytochrome c oxidase subunit 4 (COX IV) antibody [mouse anti-COX IV antibody (20E8C12)] (ab14744; Abcam PLC., Cambridge, UK; Fig. 1d). COX IV staining is recognised as a loading control for mitochondria

(3) Obesity impairs cardiolipin-dependent mitophagy and therapeutic intercellular mitochondrial transfer ability of mesenchymal stem cellsCell death & diseaseMay 13, 2023Sagar S, Faizan MI, Chaudhary N, Singh V, Singh P, Gheware A, Sharma K, Azmi I, Singh VP, Kharya G, et al.1,Abcam,ab207612 9,Anti-VDAC1/Porin,Abcam,ab15895 10,Anti-PGC1 alpha,Abcam,ab106814 11,Anti-PINK1,Abcam,ab75487 12,Anti-COX IV,Abcam,ab14744 13,Anti-FUNDC1,Abcam,ab74834 14,Anti-DRP1,Abcam,ab140494 15,Anti-SQSTM1/p62,Abcam,ab56416 16,Anti-Uteroglobin

(4) Deficits in mitochondrial TCA cycle and OXPHOS precede rod photoreceptor degeneration during chronic HIF activationMolecular neurodegenerationMarch 7, 2023Todorova V, Stauffacher MF, Ravotto L, Nötzli S, Karademir D, Ebner LJA, Imsand C, Merolla L, Hauck SM, Samardzija M, et al.}$$\end{document}∘C: rabbit anti-GLUT3 (1:250; Sigma-Aldrich, ab1344), mouse anti-COX4i1 (1:100; Abcam, ab14744), rabbit anti-CRE (1:300; Merck Millipore, 69050-3), rabbit anti-VDAC1 (1:250; Abcam, ab15895), rabbit

(5) Polynucleotide phosphorylase protects against renal tubular injury via blocking mt-dsRNA-PKR-eIF2α axisNature communicationsMarch 3, 2023Zhu Y, Zhang M, Wang W, Qu S, Liu M, Rong W, Yang W, Liang H, Zeng C, Zhu X, et al.eIF2α (CST, 5324 S, 1:1000), p-eIF2α (CST, 3398 S, 1:1000), ATF4 (Abcam, ab184909), COX IV (Abcam, ab14744, 1:1000), LaminB1 (Abcam, ab65986, 1:1000), and α-tubulin (Proteintech, 11224-1-AP, 1:2000) were used

(6) TERT Extra-Telomeric Roles: Antioxidant Activity and Mitochondrial ProtectionInternational journal of molecular sciencesFebruary 23, 2023Marinaccio J, Micheli E, Udroiu I, Di Nottia M, Carrozzo R, Baranzini N, Grimaldi A, Leone S, Moreno S, Muzzi M, et al.Santa Cruz Biotechnology), anti-Aco2 (NBP1-32781, Novus Biological), anti-CHIP (ab2917, Abcam), anti-COX IV (ab14744, Abcam) and anti-α-tubulin (T6199, Sigma-Aldrich). Finally, membranes were incubated with the appropriate

(7) Amyloid-β accumulation in human astrocytes induces mitochondrial disruption and changed energy metabolismJournal of neuroinflammationFebruary 20, 2023Zyśk M, Beretta C, Naia L, Dakhel A, Påvénius L, Brismar H, Lindskog M, Ankarcrona M, Erlandsson A.(1:200),Astrocytes,Millipore,ABD69 COX IV,Rabbit,"Immunocytochemistry (1:200), WB (1:500)",Mitochondria,Abcam,ab14744 VDAC-1,Mouse,"Immunocytochemistry (1:200), WB (1:500)",Mitochondria,Abcam,ab14734 pDRP-1 (S616),Rabbit,"Immunocytochemistry

(8) Thymidine Kinase 2 and Mitochondrial Protein COX I in the Cerebellum of Patients with Spinocerebellar Ataxia Type 31 Caused by Penta-nucleotide Repeats (TTCCA)(n)Cerebellum (London, England)February 1, 2023Aoki H, Higashi M, Okita M, Ando N, Murayama S, Ishikawa K, Yokota T.Sigma-Aldrich), mouse anti-COX IV antibody (for western blot, sc-376731 [1:1500], Santa Cruz, CA, USA; for IHC, ab14744 [1:100], Abcam), and mouse anti-COX I antibody (for western blot, ab14705 [1:5000], Abcam; for IHC,

(9) Pantothenate and L-Carnitine Supplementation Improves Pathological Alterations in Cellular Models of KAT6A SyndromeGenesDecember 6, 2022Munuera-Cabeza M, Álvarez-Córdoba M, Suárez-Rivero JM, Povea-Cabello S, Villalón-García I, Talaverón-Rey M, Suárez-Carrillo A, Reche-López D, Cilleros-Holgado P, Piñero-Pérez R, et al.(NFS1) (ab58623), superoxide dismutase 2 (SOD2) (ab68155), anti-cytochrome C oxidase subunit 4 (COX4) (ab14744), anti-nicotinamide phosphoribosyltransferase 1 (NAMPT1) (ab236874), and anti-mitochondrial ferritin

(10) A library of base editors for the precise ablation of all protein-coding genes in the mouse mitochondrial genomeNature Biomedical EngineeringDecember 5, 2022Pedro Silva-Pinheiro, Christian D. Mutti, Lindsey Van Haute, Christopher A. Powell, Pavel A. Nash, Keira Turner, Michal Minczuk1:2,000 (Abcam, ab14714); mouse anti-UQRC2, 1:1,000 (Abcam, ab14745); mouse anti-COX IV, 1:1,000 (Abcam, ab14744) and mouse anti-ATP5A, 1:1,000 (Abcam, ab14748) all diluted in 5% milk. Membranes were then washed three

(11) Targeting the Autophagy-Lysosome Pathway in a Pathophysiologically Relevant Murine Model of Reversible Heart FailureJACC. Basic to translational scienceDecember 1, 2022Evans S, Ma X, Wang X, Chen Y, Zhao C, Weinheimer CJ, Kovacs A, Finck B, Diwan A, Mann DL.University, St. Louis, Missouri, USA); Ubiquitin (ab179434, Abcam); cytochrome C oxidase-IV (COX-IV, Abcam, ab14744); and translocase of the outer mitochondrial membrane 20 (Tomm20, Sigma, MFCD04118702). Transmission electron microscopy

(12) PGC-1Î± in the myofibers regulates the balance between myogenic and adipogenic progenitors affecting muscle regenerationiScienceNovember 18, 2022Marc BeltrÃ , Fabrizio Pin, Domiziana Costamagna, Robin Duelen, Alessandra Renzini, Riccardo BallarÃ², Lorena Garcia-Castillo, Ambra Iannuzzi, Viviana Moresi, Dario Coletti, et al.antibodies directed against specific proteins: α-Tubulin (1/1000; T5168, Sigma-Aldrich), COX-IV (1/1000; ab14744, Abcam), GAPDH (1/10,000; G8795, Sigma-Aldrich), MyoG (1/500; sc-12732, Santa Cruz Biotech), Pax7 (½0;

(13) Mitochondrial Dysfunction in a High Intraocular Pressure-Induced Retinal Ischemia Minipig ModelBiomoleculesOctober 21, 2022Michael PasÃ¡k, Marie VaniÅ¡ovÃ¡, Lucie TichotovÃ¡, Jana KÅ™ÃÅ¾ovÃ¡, Taras Ardan, Yaroslav Nemesh, Jana ÄŒÃÅ¾kovÃ¡, Anastasiia Kolesnikova, Ruslan Nyshchuk, Natasha Josifovska, et al.c reductase core protein II antibody (MS304, Mitosciences 1:5000); Complex IV anti-COX IV antibody (Ab14744, Abcam; 1:1000); Complex V anti-ATP50 antibody (MS507, Mitosciences; 1:2000). Mt quality control proteins:

(14) Tissue-specific mitochondrial HIGD1C promotes oxygen sensitivity in carotid body chemoreceptorseLifeOctober 18, 2022Alba TimÃ³n-GÃ³mez, Alexandra L Scharr, Nicholas Y Wong, Erwin Ni, Arijit Roy, Min Liu, Julisia Chau, Jack L Lampert, Homza Hireed, Noah S Kim, et al.anti-β-ACTIN,Abcam,ab8227 Mouse anti-CORE2,Abcam,ab8227 Mouse anti-COX1,Abcam,ab14705 Mouse anti-COX4I1,Abcam,ab14744 Rabbit anti-COX4I2,Abnova,H00084701-M01 Mouse anti-COX5B,Santa Cruz,sc-374417 Mouse anti-β-TUBULIN,Sigma-Aldrich,C4585 Rabbit

(15) Effects of Lactate Administration on Mitochondrial Respiratory Function in Mouse Skeletal MuscleFrontiers in PhysiologyJune 30, 2022Kenya Takahashi, Yuki Tamura, Yu Kitaoka, Yutaka Matsunaga, Hideo Hatta(ubiquinone) iron-sulfur protein 4 (NDUFS4; ab139178, Abcam), cytochrome c oxidase subunit IV (COX IV; ab14744, Abcam), and CS (ab129095; Abcam). The following secondary antibodies were used in the present study:

(16) Effects of endurance training on metabolic enzyme activity and transporter protein levels in the skeletal muscles of orchiectomized miceThe journal of physiological sciences : JPSJune 29, 2022Takahashi K, Kitaoka Y, Hatta H.[ATP5F1A/ATP5A]; #ab110413; Abcam, Cambridge, UK), cytochrome c oxidase subunit 4I1 (COX4I1/COXIV; #ab14744; Abcam), glucose transporter 1 (GLUT1; #sc-377228; Santa Cruz Biotechnology, Santa Cruz, CA, USA), glucose

(17) Dynamic full-field optical coherence tomography allows live imaging of retinal pigment epithelium stress modelCommunications biologyJune 10, 2022Groux K, Verschueren A, Nanteau C, Clémençon M, Fink M, Sahel JA, Boccara C, Paques M, Reichman S, Grieve K.ATP synthase Subunit Beta Mouse Monoclonal Antibody (A21351 Life Technologies) 1/500 and mCoxIV (mouse monoclonal [20E8] ab14744 Abcam) 1/250Nuclei: Hoescht 1/1000Actin filaments: phalloidin 1/40 (10634053 Fisher Scientific)Microvilli:

(18) Increased fatty acid metabolism attenuates cardiac resistance to β-adrenoceptor activation via mitochondrial reactive oxygen species: A potential mechanism of hypoglycemia-induced myocardial injury in diabetesRedox biologyJune 1, 2022Huang L, Chen Z, Chen R, Lin L, Ren L, Zhang M, Liu L.1:1000; Abcam), complex I (COX I, cat.19703-1-AP, 1:1000; Proteintech) and complex IV (COX IV, cat.ab14744, 1:1000; Abcam). The expression of all detected proteins was normalized to that of β-actin (Cat.A2103,

(19) Compound Heterozygous COX20 Variants Impair the Function of Mitochondrial Complex IV to Cause a Syndrome Involving Ophthalmoplegia and Visual FailureFrontiers in NeurologyMay 16, 2022Peizheng Li, Dandan Guo, Xiufang Zhang, Kunqian Ji, Hongbo Lv, Yanli Zhang, Zhichao Chen, Jun Ma, Yaofeng Fang, Yiming Liumembranes were incubated with primary antibodies against COX2 (1:1,000, ab15191, Abcam) and COX4 (1:1,000, ab14744, Abcam), followed by incubation with secondary antibodies from the same species. Complex II (CII) (1:1,000,

(20) Upregulation of mitochondrial ATPase inhibitory factor 1 (ATPIF1) mediates increased glycolysis in mouse heartsThe Journal of clinical investigationMay 16, 2022Zhou B, Caudal A, Tang X, Chavez JD, McMillen TS, Keller A, Villet O, Zhao M, Liu Y, Ritterhoff J, et al.Cell Signaling Technology; OXPHOS cocktail (catalog ab110413), cytochrome c core 1 (catalog ab110252), COX-4 (catalog ab14744), and HIF1α (catalog ab1) were from Abcam; NDUFS4 (catalog MA5-19432) and COX-1 (catalog PA5-26688)

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Anti-COX IV antibody [20E8C12] from Abcam

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Anti-COX IV antibody [20E8C12] from Abcam

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