Anti-Cofilin (phospho S3) antibody ab12866 from Abcam

Product Specs
ItemAnti-Cofilin (phospho S3) antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab12866
Antibody TypePrimary
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
ClonalityPolyclonal
IsotypeIgG
HostRabbit
Quantity50 UL
Conjugate/TagUnconjugated
ModificationsPhospho-S3
Gene NameCFL1
ReactivityAfrican green monkey, Dog, Human, Mouse, Rat
TargetCofilin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) PCDH12 loss results in premature neuronal differentiation and impeded migration in a cortical organoid modelCell reportsAugust 29, 2023Rakotomamonjy J, Rylaarsdam L, Fares-Taie L, McDermott S, Davies D, Yang G, Fagbemi F, Epstein M, Fairbanks-Santana M, Rozet JM, et al.7A8.2.1, Cytoskeleton, Inc., AAN02-S), cofilin (Abcam, ab11062), and phospho-cofilin (1:1000, Abcam, ab12866). Membranes were then incubated with relevant near-infrared dyes-conjugated secondary antibodies (1:10,000,

(2) miR-137-LAPTM4B regulates cytoskeleton organization and cancer metastasis via the RhoA-LIMK-Cofilin pathway in osteosarcomaOncogenesisMay 6, 2023Yan R, Liu D, Wang J, Liu M, Guo H, Bai J, Yang S, Chang J, Yao Z, Yang Z, et al.Heavy Chain II-A antibody from Covance (Cat#PRB-440P), Cofilin (Phospho S3) antibody from Abcam (Cat#ab12866), Cofilin antibody from Abcam (Cat#ab42824), Phospho-LIMK1(Thr508)/LIMK2(Thr505) antibody from Cell Signaling Technology

(3) LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the β-catenin pathwayOpen Med (Wars)March 7, 2022Zeyi Ling, Xiaoli Long, Ying Wu, Jie Li, Mingliang Feng(ab2302) and anti-cleaved PARP (ab4830) (1:3,500; Abcam); anti-cofilin (ab42824) and anti-p-cofilin (ab12866) (1:4,000; Abcam); anti-β-catenin (ab6302), anti-β-tubulin (ab6046), and anti-Histone H3 (ab18521) (1:4,500;

(4) Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin PathwayFrontiers in medicineJanuary 1, 2022Feng L, Li H, Du Y, Zhang T, Zhu Y, Li Z, Zhao L, Wang X, Wang G, Zhou L, et al.(Proteintech, 11603-1-AP), anti-cofilin (Abcam, ab54532), anti-phosphorylated cofilin (p-cofilin, Abcam, ab12866), anti-LIMK1 (Affinity, AF6345), anti-TESK1 (Affinity, DF4012), anti-SSH1 (Abcam, ab76943), and anti-GAPDH

(5) Cervical Cancer Cells-Derived Extracellular Vesicles Containing microRNA-146a-5p Affect Actin Dynamics to Promote Cervical Cancer Metastasis by Activating the Hippo-YAP Signaling Pathway via WWC2Journal of oncologyJanuary 1, 2022Wang W, Wu L, Tian J, Yan W, Qi C, Liu W, Xuan S, Shang A.#8654, CST), p-YAP (1 : 1000, #4911, CST), cofilin (1.0 μg/mL, ab42824, Abcam), p-cofilin (1 : 1000, ab12866, Abcam), F-actin (1 : 1000, ab205, Abcam), G-actin (1 : 1000, ab123034, Abcam), and GAPDH (1 : 5000,

(6) Environmental enrichment combined with fasudil promotes motor function recovery and axonal regeneration after strokeNeural regeneration researchDecember 1, 2021Zhu YT, Zhang Q, Xie HY, Yu KW, Xu GJ, Li SY, Wu Y.hour. Membranes were then incubated overnight at 4°C with rabbit anti-p-cofilin (phosphor S3; Cat# ab12866, 1:500, Abcam, Cambridge, MA, USA), anti-cofilin (Cat# ab42824, 1:2000, Abcam), anti-p-LIM domain kinase

(7) Crosstalk Between Abnormal TSHR Signaling Activation and PTEN/PI3K in the Dedifferentiation of Thyroid Cancer CellsFrontiers in OncologySeptember 28, 2021Fang Feng, Huiqin Han, Shuqi Wu, Hui Wangprotein (S235/236) rabbit monoclonal (Cell Signaling #4858S) at 1:1000, anti-Phospho-cofilin (Abcam #ab12866) at 1:1000 and anti-GAPDH rabbit monoclonal (Cell Signaling #2118) at 1:20000 dilution. Blots were scanned

(8) Mirtronic miR-4646-5p promotes gastric cancer metastasis by regulating ABHD16A and metabolite lysophosphatidylserinesCell death and differentiationSeptember 1, 2021Yang L, Hou Y, Du YE, Li Q, Zhou F, Li Y, Zeng H, Jin T, Wan X, Guan S, et al.anti-p-LIMK1 (1:1000; ab38508, Abcam), anti-cofilin (1:1000; ab42824, Abcam), anti-p-cofilin (1:1000; ab12866, Abcam) and anti-β-Actin (1:1000; Bioshap). Next, the membranes were incubated in a secondary antibody

(9) MiR-509-3p Induces Apoptosis and Affects the Chemosensitivity of Cervical Cancer Cells by Targeting the RAC1/PAK1/LIMK1/Cofilin PathwayChemical and Pharmaceutical BulletinApril 1, 2021Jia Xu, Xiangdong Ma, Hong Yang, Junru Zhang, Guoqing Cai, Nianling Yaoanti-Coﬁlin antibody (ab54532), and anti-Coﬁlin 332 Chem. Pharm. Bull. (phospho S3) antibody (ab12866). After being rinsed with TBST, the membranes were incubated for one hour with horseradish peroxidase

(10) The common YAP activation mediates corneal epithelial regeneration and repair with different-sized woundsNPJ Regenerative medicineMarch 26, 2021Li Y, Ge L, Chen X, Mao Y, Gu X, Ren B, Zeng Y, Chen M, Chen S, Liu J, et al.CK15 (Abcam, ab80522; 1:3000), rabbit Ki67 (Abcam, ab15580; 1:800), rabbit p-Cofilin (Ser3; Abcam, ab12866; 1:2000), rabbit Cofilin (Abcam, ab42824; 1:2500), rabbit p-LIMK (LIMK1 Thr 508, LIMK2 Thr 505; CST,

(11) Systemic HDAC3 inhibition ameliorates impairments in synaptic plasticity caused by simulated galactic cosmic radiation exposure in male miceNeurobiology of Learning and MemoryFebruary 1, 2021A.A. Keiser, E.A. Kramár, T. Dong, S. Shanur, M. Pirodan, N. Ru, M.M. Acharya, J.E. Baulch, C.L. Limoli, M.A. Woodbuffer and were then incubated in primary antibodies (1:1000, rabbit anti-cofilin (phospho S3), Abcam, ab12866); (1:5000, mouse anti- ß-Actin (AC-15), Abcam, ab6276) in 1.5% BSA/washing buffer overnight at 4 °C

(12) Environmental enrichment combined with fasudil treatment inhibits neuronal death in the hippocampal CA1 region and ameliorates memory deficitsNeural Regeneration ResearchJanuary 12, 2021Gao-Jing Xie, Qun Zhang, Gao-Jing Xu, Yi-Tong Zhang, Si-Yue Li, Chuan-Jie Wang, Hong-Yu Xie, Chuan-Jie Wang(1:1000; rabbit; ab42824, Abcam, Cambridge, MA, USA), anti-cofilin (phospho S3) antibody (1:1000; rabbit, ab12866, Abcam), and anti-beta-actin antibody (1:1000; rabbit; ab8227, Abcam). The membranes were then incubated

(13) Cyclovirobuxine D Induces Apoptosis and Mitochondrial Damage in Glioblastoma Cells Through ROS-Mediated Mitochondrial Translocation of CofilinFrontiers in oncologyJanuary 1, 2021Zhang L, Fu R, Duan D, Li Z, Li B, Ming Y, Li L, Ni R, Chen J.Cell Signaling Technology (Beverly, MA, USA); cofilin (Cat.no. ab42824), phospho-Cofilin (S3, Cat.no. ab12866), VDAC1 (Cat.no. ab14734), phospho-ERK (Cat.no. ab50011), AKT (Cat.no. ab200195) and ERK (Cat.no. ab17942)were

(14) ROCK1 activation-mediated mitochondrial translocation of Drp1 and cofilin are required for arnidiol-induced mitochondrial fission and apoptosisJournal of Experimental & Clinical Cancer ResearchDecember 1, 2020Jinjiao Hu, Hongwei Zhang, Jie Li, Xiuxing Jiang, Yanhao Zhang, Qin Wu, liwen Shen, Jingshan Shi, Ning GaoPARP (1078–1) was purchased from Epitomics (Burlingame, USA); ROCK1 (ab45171), phospho-Cofilin (S3, ab12866) were purchased from Abcam (Cambridge, UK); PP2A (610555) was purchased from BD Biosciences (Franklin,

(15) LINC00452 promotes ovarian carcinogenesis through increasing ROCK1 by sponging miR-501-3p and suppressing ubiquitin-mediated degradationAgingNovember 9, 2020Yang J, Wang WG, Zhang KQ.anti-phosphorylated (p)-LIMK1 (Abcam, #ab194798), anti-LIMK1 (Abcam, #ab81046), anti-p-cofilin (Abcam, #ab12866), anti-cofilin (Abcam, # ab42824) and anti-β-actin (Santa Cruz, #sc-47778) antibodies. Luciferase reporter assay

(16) Polyphyllin II inhibits liver cancer cell proliferation, migration and invasion through downregulated cofilin activity and the AKT/NF-κB pathwayBiology OpenFebruary 7, 2020Dejiang Pang, Chengcheng Yang, Chao Li, Yuanfeng Zou, Bin Feng, Lixia Li, Wentao Liu, Qihui Luo, Zhengli Chen, Chao Huang(S536) (Abcam, ab28856, 1:1000 dilution), Cofilin (Abcam, ab42824, 1:1000 dilution), pCofilin (Abcam, ab12866, 1:1000 dilution), LIMK1 (Abcam, ab108507, 8 Biology Open Western blotting analysis Cell viability assay

(17) Effects of Dexamethasone on Remodeling of the Hippocampal Synaptic Filamentous Actin Cytoskeleton in a Model of Pilocarpine-induced Status EpilepticusInternational journal of medical sciencesJanuary 1, 2020Yang N, Zhang Y, Wang JT, Chen C, Song Y, Liang JM, Ma DH, Zhang YF.primary antibodies included rabbit polyclonal anti-phosphorylated-cofilin (pCofilin) at serine 3 (1:500, ab12866, Abcam, USA) and mouse anti-beta-actin (1:2000, A2228, Sigma Aldrich, MO, USA). For quantification of

(18) S-Nitrosylation of Plastin-3 Exacerbates Thoracic Aortic Dissection Formation via Endothelial Barrier DysfunctionArteriosclerosis, Thrombosis, and Vascular BiologyJanuary 1, 2020Lihong Pan et al.CST, Danvers, MA), anti-plectin (no. ab32528, Abcam), anti-cofilin (no. ab11062, Abcam), anti-p-cofilin (no. ab12866, Abcam), anti-PLS3 (no. ab137585, Abcam), anti-iNOS (no. ab3523, Abcam), anti-eNOS (endothelial nitric

(19) Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat basolateral amygdalaLearning & memory (Cold Spring Harbor, N.Y.)November 1, 2019Bessières B, Jia M, Travaglia A, Alberini CM.cat# AB5406), Gephyrin (1:1000, Synaptic Systems, cat# 147-111), p-coﬁlin (Ser3) (1:1000, Abcam, cat# ab12866), coﬁlin (1:1000, Millipore, cat# AB3842), MAP2 (1:1000, Millipore, cat# MAB3418), mTOR (1:1000, Cell Signaling Technology

(20) Invadopodia are chemosensing protrusions that guide cancer cell extravasation to promote brain tropism in metastasisOncogeneMay 1, 2019Karla C. Williams et al.Cell Signaling Technology 2601S, Anti-PAK1 antibody Abcam ab40852, Cofilin (phospho S3) antibody Abcam ab12866, Anti-Cofilin antibody (ab42824) Abcam ab42824. All secondary antibodies and Alexa Fluor 647–labeled

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Anti-Cofilin (phospho S3) antibody from Abcam

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Anti-Cofilin (phospho S3) antibody from Abcam

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