Fig 1: POGZ binds euchromatic loci and HP1? in human fetal cortex(A) ISH of POGZ antisense probe (purple) in 18 gw fetal cortex tissue(B) Co-IPs for HP1a and HP1? from nuclear extracts of 18 gw cortex.(C) Heatmap of POGZ, HP1g, and IgG control C&R reads across POGZ consensus peaks (n = 9,089) in human mid-fetal cortex. ATAC-seq and H3K27ac and H4K20me3 ChIP-seq reads from mid-fetal cortex samples (Markenscoff-Papadimitriou et al., 2020). RPM reads per million.(D) Gene set enrichment analysis for genes proximal (within 50 kb) to POGZ C&R peaks, H4K20me3 peaks as control. Gene sets that are significantly enriched in POGZ C&R-proximal genes are indicated with a star (q-value < 0.001 after multiple testing correction).
Fig 2: CBX3 expression level in osteosarcoma tissues was significantly higher than this level in corresponding non-cancerous bone tissues. *P<0.05 compared with the paired adjacent bone tissues. CBX3, chromobox protein homolog 3.
Fig 3: CBX3 modulates the cell cycle distribution and apoptosis of osteosarcoma MG63 cells. (A) Flow cytometric analysis of cell cycle distribution of the MG63 cells transfected with CBX3 siRNA or NC siRNA. (B) Flow cytometric analysis of the percentage of apoptotic MG63 cells transfected with CBX3 siRNA or NC siRNA. *P<0.05 compared with the NC siRNA group. CBX3, chromobox protein homolog 3.
Fig 4: CBX3 siRNA was constructed to inhibit the proliferation of osteosarcoma cells. The knockdown efficiency of CBX3 was assessed by (A) western blotting and (B) qPCR assay, respectively. (C) CCK-8 assay shows that knockdown of CBX3 by CBX3 siRNA decreased the proliferation of osteosarcoma MG63 cells when compared to the NC siRNA group. *P<0.05 compared with the NC siRNA group. CBX3, chromobox protein homolog 3; CCK-8, Cell-Counting Kit-8.
Fig 5: CBX3 is overexpressed in sarcoma tissues. CBX3 mRNA expression is significantly upregulated in (A) dedifferentiated liposarcoma, (B) myxoid/round cell liposarcoma, (C) myxofibrosarcoma and (D) pleomorphic liposarcoma compared with that in paired normal tissues. Data were collected and analyzed via the Oncomine database. CBX3, chromobox protein homolog 3.
Supplier Page from Abcam for Anti-HP1 gamma/CBX3 antibody