Anti-Endothelial Cell antibody [RECA-1] ab9774 from Abcam

Product Specs
ItemAnti-Endothelial Cell antibody [RECA-1]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab9774
Antibody TypePrimary
Quantity10 UG, 100 UG
ClonalityMonoclonal
HostMouse, Nude mouse
IsotypeIgG1
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry - Free Floating, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Immunohistochemistry (Glycolmethacrylate sections), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry - Free Floating, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Immunohistochemistry (Glycolmethacrylate sections)
CloneRECA-1
Conjugate/TagUnconjugated
ReactivityRat
TargetEndothelial Cell

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(21) Co‐overexpression of VEGF and GDNF in adipose‐derived stem cells optimizes therapeutic effect in neurogenic erectile dysfunction modelCell ProliferationFebruary 1, 2020Wende Yang, Zehong Chen, Xiaolei Ma, Xi Ouyang, Jiafeng Fang, Hongbo Weianti‐Desmin (1:200, Abcam, ab32362), anti‐HIF‐1α (1:50, Abcam, ab179483), anti‐RECA‐1 (1:50, Abcam, ab9774), anti‐VEGF (1:100, Affinity, AF5131) and anti‐GDNF (1:100, Servicebio, gb11403). DyLight 488‐ and 556‐conjugated

(22) Gene expression and growth factor analysis in early nerve regeneration following segmental nerve defect reconstruction with a mesenchymal stromal cell-enhanced decellularized nerve allograftPlastic and Reconstructive Surgery - Global OpenJanuary 21, 2020Nadia Rbia, Liselotte F. Bulstra, Patricia F. Friedrich, Allen T. Bishop, Tim H.J. Nijhuis, Alexander Y. Shin1:5,000; polyclonal PGP9.5 anti-rabbit (Dako Z5116) was used at 1:500; monoclonal RECA-1 anti–mouse (Abcam ab9774) was used at 1:200; monoclonal NGF anti-rabbit (Abcam ab52918) was used at 1:150 and polyclonal anti-rabbit

(23) Tuberous Sclerosis (tsc2+/-) Model Eker Rats Reveals Extensive Neuronal Loss with Microglial Invasion and Vascular Remodeling Related to Brain NeoplasiaNeurotherapeuticsJanuary 1, 2020Viera Kútna, Libor Uttl, Robert Waltereit, Zdenka Krištofiková, Daniel Kaping, Tomáš Petrásek, Cyril Hoschl, Saak V. Ovsepiancatalog# AB5733), and anti-Endothelial cell antibody (RECA-1, mouse monoclonal, 1:400, Abcam, catalog# ab9774). Before incubation with anti-Nestin antibody (rabbit monoclonal, 1:100, Abcam, catalog# ab221660),

(24) Rat Mesenchymal Stromal Cell Sheets Suppress Renal Fibrosis via Microvascular ProtectionStem cells translational medicineDecember 1, 2019Imafuku A, Oka M, Miyabe Y, Sekiya S, Nitta K, Shimizu T.antibody (M0851, Dako, Carpinteria, CA, USA), rat antiendothelial cell antigen‐1 (RECA‐1) antibody (ab9774, Abcam, Cambridge, UK), and rabbit anti‐phospho‐cMet antibody (ab5662; Abcam), detected using peroxidase‐conjugated

(25) Effect of extradural constriction on CSF flow in rat spinal cordFluids and Barriers of the CNSDecember 1, 2019Joel A. Berliner, Thomas Woodcock, Elmira Najafi, Sarah J. Hemley, Magdalena Lam, Shaokoon Cheng, Lynne E. Bilston, Marcus A. StoodleyEndothelial cells were identified using a mouse anti-endothelial cell monoclonal antibody (1:100; RECA-1, ab9774, Abcam, Australia); smooth muscle cells of arteries and arterioles were identified using a mouse monoclonal

(26) Renal subcapsular transplantation of hepatocyte growth factor-producing mesothelial cell sheets improves ischemia-reperfusion injuryAmerican Journal of Physiology-Renal PhysiologyAugust 1, 2019Yoei Miyabe, Sachiko Sekiya, Naoko Sugiura, Masatoshi Oka, Kazunori Karasawa, Takahito Moriyama, Kosaku Nitta, Tatsuya ShimizuFirst, DAB staining was performed on paraffin-embedded sections with anti-RECA-1 antibody (catalog no. ab9774, Abcam), which is a marker for endothelial cells, and five pictures were taken randomly in the area;

(27) Hepatocyte Growth Factor–Secreting Mesothelial Cell Sheets Suppress Progressive Fibrosis in a Rat Model of CKDJournal of the American Society of NephrologyFebruary 1, 2019Masatoshi Oka, Sachiko Sekiya, Ryoichi Sakiyama, Tatsuya Shimizu, Kosaku Nitta-smooth muscle actin (aSMA; M0851; Dako), SV40 T antigen (ab16879; Abcam), rat endothelial cell antigen-1 (ab9774; Abcam), or hHGF (provided by Kunio Matsumoto) and detected using a peroxidase-conjugated polymer reagent

(28) Physical positioning markedly enhances brain transduction after intrathecal AAV9 infusionScience advancesNovember 1, 2018Castle MJ, Cheng Y, Asokan A, Tuszynski MH.MilliporeSigma #A2052), mouse monoclonal anti–rat endothelial cell antigen-1 (RECA-1) (1:100; Abcam #ab9774), mouse monoclonal anti–tyrosine hydroxylase (TH) (1:1000; MilliporeSigma #MAB318), rabbit polyclonal

(29) Biodegradation of ECM hydrogel promotes endogenous brain tissue restoration in a rat model of stroke.Acta BiomaterialiaOctober 1, 2018Harmanvir Ghuman, Carrinton Mauney, Julia Donnelly, Andre R. Massensini, Stephen F. Badylak, Michel Modoand AAT58219.1 CNPase,1:200,Abcam,Ab6319,11–5B Fox3,1:500,Abcam,Ab104224,1B7 RECA-1,1:100,Abcam,Ab9774,RECA-1 CD86,1:200,Abcam,Ab53004,EP1159Y CD206,1:200,Santa Cruz,sc-34577,C-20 List of antibodies

(30) Delivery of immunoglobulin G antibodies to the rat nervous system following intranasal administration: Distribution, dose-response, and mechanisms of deliveryJournal of controlled release : official journal of the Controlled Release SocietySeptember 28, 2018Kumar NN, Lochhead JJ, Pizzo ME, Nehra G, Boroumand S, Greene G, Thorne RG.included the following: mouse anti-rat endothelial cell antigen-1 (RECA-1) monoclonal antibody (Abcam ab9774; 1:500 dilution), rabbit anti-protein gene product 9.5 (PGP 9.5) polyclonal antibody (Abcam ab27053;

(31) Morphometric analysis of astrocytes in brainstem respiratory regionsThe Journal of comparative neurologySeptember 1, 2018SheikhBahaei S, Morris B, Collina J, Anjum S, Znati S, Gamarra J, Zhang R, Gourine AV, Smith JC.al., 2010, 2013, 2016; Marchenko et al., 2016). The mouse monoclonal RECA‐1 (1:1000, Abcam, catalog #ab9774, RRID: http://scicrunch.org/resolver/AB_296613) antibody was raised against peripheral and mesenteric

(32) An allogeneic ‘off the shelf’ therapeutic strategy for peripheral nerve tissue engineering using clinical grade human neural stem cellsScientific ReportsFebruary 13, 2018C. O’Rourke, A. G. E. Day, C. Murray-Dunning, L. Thanabalasundaram, J. Cowan, L. Stevanato, N. Grace, G. Cameron, R. A. L. Drake, J. Sinden, J. B. Phillips

(33) Neural precursor cells form integrated brain-like tissue when implanted into rat cerebrospinal fluidCommunications biologyJanuary 1, 2018Pothayee N, Maric D, Sharer K, Tao-Cheng JH, Calac A, Bouraoud N, Pickel J, Dodd S, Koretsky A.number are indicated in parentheses): mouse IgG1 anti-rat endothelial cell antigen (RECA1) (Abcam, ab9774), mouse IgG1 anti-CD68/ED1 (Thermo Fisher Scientific, MA5-16654), mouse IgG1-calretinin (EMD Millipore,

(34) Bevacizumab Improves Achilles Tendon Repair in a Rat ModelCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyJanuary 1, 2018Tempfer H, Kaser-Eichberger A, Lehner C, Gehwolf R, Korntner S, Kunkel N, Wagner A, Gruetz M, Heindl LM, Schroedl F, et al.triple immunohistochemistry (overnight at 4°C) with antibodies against rat endothelial cell antigen (RECA-1 #AB9774, Abcam, Cambridge, UK; 1:500), CD68 (#sc20060, Santa Cruz, Dallas, USA; 1:50), lymphatic vessel endothelial

(35) The ultrastructure of spinal cord perivascular spaces: Implications for the circulation of cerebrospinal fluidScientific ReportsDecember 1, 2017Magdalena A. Lam, Sarah J. Hemley, Elmira Najafi, Nicole G. F. Vella, Lynne E. Bilston, Marcus A. Stoodleywalls of blood vessels, sections were incubated with Rat Endothelial Cell Antibody (1:100; RECA-1; AB9774 Abcam, Cambridge, United Kingdom) followed by a secondary antibody (1:400; anti-mouse IgG Alexa Fluor

(36) Long-term retention of ECM hydrogel after implantation into a sub-acute stroke cavity reduces lesion volumeActa BiomaterialiaNovember 1, 2017Harmanvir Ghuman, Madeline Gerwig, Francesca J. Nicholls, Jessie R. Liu, Julia Donnelly, Stephen F. Badylak, Michel Modoand AAT58219.1 CNPase,1:200,Abcam,Ab6319,11-5B Fox3,1:500,Abcam,Ab104224,1B7 RECA-1,1:100,Abcam,Ab9774,RECA-1 CD86,1:200,Abcam,Ab53004,EP1159Y CD206,1:200,Santa Cruz,sc-34577,C-20 List of antibodies

(37) Localized Down-regulation of P-glycoprotein by Focused Ultrasound and Microbubbles induced Blood-Brain Barrier Disruption in Rat BrainScientific ReportsNovember 1, 2016HongSeok Cho, Hwa-Youn Lee, Mun Han, Jong-ryul Choi, Sanghyun Ahn, Taekwan Lee, Yongmin Chang, Juyoung Parkand incubated for 16 hours at 4 degree in mouse anti-P-gp (C219, Enzo, New York, USA) or anti-RECA-1 (ab9774, Abcam, Cambridge, UK) 1:50 diluted in PBST. After thorough washing in PBST, slices were incubated in

(38) Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cellsScientific reportsMarch 16, 2016Zou L, Chen Q, Quanbeck Z, Bechtold JE, Kaufman DS.(M186, Takara), rabbit-anti-rat collagen-II Ab (AB2036, Millipore) or mouse-anti-rat endothelial cells (ab9774, Abcam), before being incubated with secondary antibody conjugated with Alexa-Fluro-488 (A-11008, or

(39) DNA Subtraction of In Vivo Selected Phage Repertoires for Efficient Peptide Pathology Biomarker Identification in Neuroinflammation Multiple Sclerosis ModelBiomarker InsightsFebruary 16, 2016Karina Vargas-Sanchez, Antonios Vekris, Klaus G. Petrypolyclonal to M13 bacteriophage (M13pVIII; Abcam ab6188) and 2 µg/mL mouse monoclonal antibody RECA-1 (Abcam ab9774) to endothelial cells for two hours at RT. Tissues were washed three times for 10 minutes and thereafter

(40) Potent spinal parenchymal AAV9-mediated gene delivery by subpial injection in adult rats and pigsMolecular therapy. Methods & clinical developmentJanuary 1, 2016Miyanohara A, Kamizato K, Juhas S, Juhasova J, Navarro M, Marsala S, Lukacova N, Hruska-Plochan M, Curtis E, Gabel B, et al.

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2/17/2022RECA-1 Antibody Suitable for IHC I RIbeiroRECA-1 can be used for staining of rat endothelial cells. We tested for IHC (IF) in rat samples.

Anti-Endothelial Cell antibody [RECA-1] from Abcam

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Anti-Endothelial Cell antibody [RECA-1] from Abcam

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