Fig 1: Grading of immunostaining of human corneal endothelial cells (CECs) using epifluorescence microscopy.(A) Labeling of Neural Cell Adhesion Molecule (NCAM) with the MAB24081 from R&D systems gave a bright homogeneous staining of all CECs and had a clear subcellular localization in the lateral membranes. Left panel, low magnification, scale bar = 200 μm. Right panel, high magnification, scale bar = 5 μm. (Thirty-six years old patient with a keratoconus). (B) Labeling of aquaporin 1 with the ab9566 from Abcam was too heterogeneous and had no clear subcellular localization to be considered as reliable. Left panel, low magnification, scale bar = 50 μm. Right panel, high magnification, scale bar = 5 μm. (Sixteen years old patient with a keratoconus).