Anti-hCG beta antibody [5H4-E2] ab9582 from Abcam

Product Specs
ItemAnti-hCG beta antibody [5H4-E2]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab9582
Antibody TypePrimary
HostMouse, Nude mouse
ApplicationsELISA, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Western blot
IsotypeIgG1
Quantity100 UG
Clone5H4-E2
ClonalityMonoclonal
Conjugate/TagUnconjugated
Concentration0.2 mg/ml
Gene NameCGB3
ReactivityHuman
TargethCG beta

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Development of a human iPSC-derived placental barrier-on-chip modeliScienceJuly 21, 2023Agathe Lermant, Gwenaëlle Rabussier, Henriëtte L. Lanz, Lindsay Davidson, Iain M. Porter, Colin E. MurdochAB_10989596 Goat polyclonal anti-GATA-3,R&D Systems,Cat#AF2605 Mouse monoclonal anti-hCG beta (5H4-E2),Abcam,Cat#ab9582 Rabbit monoclonal anti-E-Cadherin (24E10),Cell Signaling Technology,Cat#3195 Donkey anti-Goat IgG

(2) A placental model of SARS-CoV-2 infection reveals ACE2-dependent susceptibility and differentiation impairment in syncytiotrophoblastsNature Cell BiologyJuly 13, 2023J. Chen, J. A. Neil, J. P. Tan, R. Rudraraju, M. Mohenska, Y. B. Y. Sun, E. Walters, N. G. Bediaga, G. Sun, Y. Zhou, et al.temperature. The sections were then incubated with primary antibodies (ACE2, ab15438, Abcam, 1:200; HCG beta, ab9582, Abcam, 1:200; HLA-G, ab7759, Abcam, 1:50) for 1 h at 37 °C; rabbit or mouse IgG (X0936 and X0931 respectively,

(3) Laminin switches terminal differentiation fate of human trophoblast stem cells under chemically defined culture conditionsJournal of Biological ChemistryMay 1, 2023Victoria Karakis, Mahe Jabeen, John W. Britt, Abigail Cordiner, Adam Mischler, Feng Li, Adriana San Miguel, Balaji M. Rao(38),RRID:CVCL_IT66 Antibodies,, Anti-KRT7,Cell Signaling Technologies,"Cat#4465, RRID:AB_11178382" Anti-hCG,Abcam,"Cat#ab9582, RRID:AB_296507" Anti-P63,Cell Signaling Technologies,"Cat#13109, RRID:AB_2637091" Anti-TEAD4,Abcam,"Cat#ab58310,

(4) Thyroid hormone enhances stem cell maintenance and promotes lineage-specific differentiation in human embryonic stem cellsStem Cell Research & TherapyDecember 1, 2022Chunhao Deng, Zhaoying Zhang, Faxiang Xu, Jiaqi Xu, Zhili Ren, Carlos Godoy-Parejo, Xia Xiao, Weiwei Liu, Zhou Zhou, Guokai Chenflow cytometer (Beckman Coulter). Primary antibodies used include NANOG (R&D, AF1997) and CGB (Abcam, AB9582). Cell cycle analysis Cell cycle analysis was conducted using Click-iT® EdU Flow Cytometry Assay Kits

(5) Protocol to derive human trophoblast stem cells directly from primed pluripotent stem cellsSTAR protocolsSeptember 16, 2022Wei Y, Xiao L, Ma L, Wang Z, Huang L, Li H, Pan G, Lye SJ, Shan Y.,Santa Cruz Biotechnology,Cat#sc-5279 HLA-G (1:500 dilution),Novus Biologicals,Cat#NB500-314 CGB (1:500 dilution),Abcam,Cat#ab9582 "Goat anti-Rabbit IgG (H+L), Alexa Fluor 488(1:500 dilution)",Thermo Fisher Scientific,Cat#A-11008 "Goat

(6) S100P promotes trophoblast syncytialization during early placenta development by regulating YAP1Front Endocrinol (Lausanne)September 14, 2022Hanjing Zhou et al.overnight at 4°C. The following primary antibodies were used: S100P (ab133554, 1:100, Abcam, USA), β-hCG (ab9582, 1:50, Abcam, USA), YAP1 (sc101199, 1:50, Santa Cruz, USA), TEAD4 (ab58310, 1:50, Abcam, USA), HLA-G

(7) Calnexin Is Involved in Forskolin-Induced Syncytialization in Cytotrophoblast Model BeWo CellsBiomoleculesJuly 28, 2022Matsukawa H, Ikezaki M, Nishioka K, Iwahashi N, Fujimoto M, Nishitsuji K, Ihara Y, Ino K.antibodies were purchased from Proteintech (Cat No. 11615-1-AP, Rosemont, IL, USA) and Abcam (Cat No. ab9582, Cambridge, UK), respectively. Rabbit polyclonal and mouse monoclonal anti-luteinizing hormone/chorionic

(8) Establishment of human induced trophoblast stem-like cells from term villous cytotrophoblastsStem cell researchOctober 1, 2021Bai T, Peng CY, Aneas I, Sakabe N, Requena DF, Billstrand C, Nobrega M, Ober C, Parast M, Kessler JA.(1:150, Biocare Medical, cat#ACI3066A), HLA-G (1:6000, Abcam, cat#ab52455) and hCG (1:300, Abcam, cat#ab9582), and detected using an HRP-based reaction with the tyramide Alexa Fluor fluorescence system (ThermoFisher)

(9) Human naive epiblast cells possess unrestricted lineage potentialCell stem cellJune 3, 2021Guo G, Stirparo GG, Strawbridge SE, Spindlow D, Yang J, Clarke J, Dattani A, Yanagida A, Li MA, Myers S, et al.RRID:AB_2783822 CK18,Abcam,Cat#AB133263; RRID:AB_11155892 HLA-G,Abcam,Cat#AB52455; RRID:AB_880552 hCGB,Abcam,Cat#AB9582; RRID:AB_296507 PKCi,Novus Biologicals,Cat#NBP1-84959; RRID:AB_11033145 PAR6B,Santa Cruz,Cat#sc-166405;

(10) HIRA contributes to zygote formation in mice and is implicated in human 1PN zygote phenotypeReproduction (Cambridge, England)May 10, 2021Smith R, Pickering SJ, Kopakaki A, Thong KJ, Anderson RA, Lin CJ.ab3349). As a negative control the antibodies raised in rabbit were incubated with mouse anti hCG (Abcam ab9582) which should not be present in mouse tissues. All wash steps were with wash buffer A. After washing,

(11) Two distinct trophectoderm lineage stem cells from human pluripotent stem cellsThe Journal of biological chemistryJanuary 1, 2021Mischler A, Karakis V, Mahinthakumar J, Carberry CK, San Miguel A, Rager JE, Fry RC, Rao BM.RRID:AB_2265604" Anti-KRT7,Cell Signaling Technologies,"Cat# 4465, RRID:AB_11178382" Anti-hCG,Abcam,"Cat# ab9582, RRID:AB_296507" Anti-hCG,Abcam,"Cat# ab9376, RRID:AB_307221" Anti-P63,Cell Signaling Technologies,"Cat#

(12) Single-cell RNA sequencing reveals regulatory mechanism for trophoblast cell-fate divergence in human peri-implantation conceptusesPLOS BiologyOctober 9, 2019Bo Lv, Qin An, Qiao Zeng, Xunyi Zhang, Ping Lu, Yanqiu Wang, Xianmin Zhu, Yazhong Ji, Guoping Fan, Zhigang Xuetemperature in 5% BSA in PBS for 1 hour and incubated with primary antibodies (hCGβ, Abcam, United Kingdom, ab9582; TBX3, Abcam, United Kingdom, ab99302; HLA-G, Abcam, United Kingdom, ab52454; OCT4, Santa Cruz Biotechnology,

(13) Establishment of human trophoblast stem cells from human induced pluripotent stem cell-derived cystic cells under micromesh cultureStem cell research & therapyAugust 7, 2019Li Z, Kurosawa O, Iwata H.primary antibodies overnight at 4 °C. The following primary antibodies were used: anti-hCG (cat. no. ab9582; 1:100; Abcam, Cambridge, UK), anti-E-cadherin (cat. no. 3195; 1:100; Cell Signaling Technology, Danvers,

(14) Efficient production of trophoblast lineage cells from human induced pluripotent stem cellsLaboratory investigation; a journal of technical methods and pathologyOctober 1, 2017Kojima J, Fukuda A, Taira H, Kawasaki T, Ito H, Kuji N, Isaka K, Umezawa A, Akutsu H.Triton X-100 (Sigma) for 10 min at room temperature. After washing with PBS, cells were incubated with HCG (ab9582; Abcam, 1:400) in 3% bovine serum albumin with PBS for 40 min at room temperature. Cells were then incubated

(15) BRCA1 regulation on β-hCG: a mechanism for tumorigenicity in BRCA1 defective breast cancerOncogenesisSeptember 4, 2017S K Sengodan et al.microscope. Antibodies Antibodies used for all the experiments were as follows: for immunoflourescence, β-hCG (ab9582, Abcam), Vimentin (5144 S, Cell signaling technology), E-cadherin (3195 S, Cell signaling technology),

(16) Real-Time Tracking of BODIPY-C12 Long-Chain Fatty Acid in Human Term Placenta Reveals Unique Lipid Dynamics in Cytotrophoblast CellsPloS oneJanuary 1, 2016Kolahi K, Louey S, Varlamov O, Thornburg K.syncytial fragments was determined by staining for Vimentin (PA5-27231, Thermo Scientific) and BHCG (ab9582, Abcam). Our isolations comprised at least >90% pure, viable cytotrophoblasts. Cytotrophoblasts were

(17) MTA3 regulates differentiation of human cytotrophoblast stem cellsPlacentaSeptember 1, 2015Mariko Horii, Matteo Moretto-Zita, Katharine K Nelson, Yingchun Li, Mana M Parast

(18) Role of IGF2BP3 in trophoblast cell invasion and migrationCell death & diseaseJanuary 23, 2014Li W, Liu D, Chang W, Lu X, Wang YL, Wang H, Zhu C, Lin HY, Zhang Y, Zhou J, et al.antibodies against IGF2BP3 (1 : 400; 14642-1-AP, Proteintech, Chicago, IL, USA) or β-hCG (1 : 200; ab9582, Abcam, Cambridge, MA, USA) at 4°C overnight. The IGF2BP3-treated sections were then incubated with

(19) BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ΔNp63+ cytotrophoblast stem cell stateDevelopment (Cambridge, England)October 1, 2013Li Y, Moretto-Zita M, Soncin F, Wakeland A, Wolfe L, Leon-Garcia S, Pandian R, Pizzo D, Cui L, Nazor K, et al.

(20) CUL1 promotes trophoblast cell invasion at the maternal–fetal interfaceCell Death & DiseaseFebruary 21, 2013Q Zhang, Q Chen, X Lu, Z Zhou, H Zhang, H-Y Lin, E Duan, C Zhu, Y Tan, H WangSanta Cruz), cleaved caspase 3 (1 : 500, 9664, Cell Signaling Technology, Beverly, MA, USA), β-hCG (1 : 500, ab9582, Abcam), β-actin (1 : 2000, 4970, Cell Signaling Technology) and GAPDH (1 : 20000, ab37187, Abcam) overnight

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Anti-hCG beta antibody [5H4-E2] from Abcam

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Anti-hCG beta antibody [5H4-E2] from Abcam

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