AgeI-HF from New England Biolabs

Product Specs
ItemAgeI-HF
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR3552S
Quantity300 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Chemically induced revitalization of damaged hepatocytes for regenerative liver repairiScienceDecember 15, 2023Pengyan Lin, Yunfei Bai, Xinxin Nian, Jun Chi, Tianzhe Chen, Jing Zhang, Wenpeng Zhang, Bin Zhou, Yang Liu, Yang Zhao,Thermo fisher,Cat#15568025 EGTA,Sigma,Cat#67-42-5 DNaseI,Sigma-Aldrich,Cat#D5025 Tamoxifen,Sigma,Cat#10540-29 Salt-T4® DNA Ligase,NEB,Cat#M0467S Age I-HF,NEB,Cat#R3552S EcoR I-HF,NEB,Cat#R3101T DDC,"Research Diets, inc.",Cat#D111122201 CCl4,Rhawnseal,Cat#RH416503 PEG300,Sigma-Aldrich,Cat#8074841000 Tween

(2) A Novel Metastatic Estrogen Receptor-Expressing Breast Cancer Model with Antiestrogen ResponsivenessCancers (Basel)December 9, 2023Kendall L. Langsten, Lihong Shi, Adam S. Wilson, Salvatore Lumia, Brian Westwood, Alexandra M. Skeen, Maria T. Xie, Victoria E. Surratt, JoLyn Turner, Carl D. Langefeld, et al.#R3189L, 20K U/mL, Lot 10030794), NruI-HF (NEB #R3192S, 20K U/mL, Lot 10030601), and AgeI-HF (NEB #R3552S, 20K U/mL, Lot 10028839) was performed with a CutSmart Buffer at 37 °C for 5–15 min or overnight to

(3) Systematic characterization of the local evolutionary space available to human PKR and vaccinia virus K3bioRxivNovember 25, 2023Michael J. Chambers, Sophia Scobell, Meru J. Sadhu55°C, followed by gel purification. YCp50-RD was digested with restriction enzymes AgeI-HF (NEB Cat#R3552S) and BstEII-HF (NEB Cat#R3162S), followed by gel purification. The digested YCp50-RD and pGAL/CYC1-PKR

(4) A quinolin-8-ol sub-millimolar inhibitor of UGGT, the ER glycoprotein folding quality control checkpointiScienceOctober 20, 2023Kevin P. Guay, Roberta Ibba, J.L. Kiappes, Snežana Vasiljević, Francesco Bonì, Maria De Benedictis, Ilaria Zeni, James D. Le Cornu, Mario Hensen, Anu V. Chandran, et al.recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, AgeI-HF,New England Biolabs,Cat# R3552S KpnI-HF,New England Biolabs,Cat# R3142S CutSmart Buffer,New England Biolabs,Cat# B7204 actually replaced

(5) An efficient cloning method to expand vector and restriction site compatibility of Golden Gate AssemblyCell Reports MethodsAugust 22, 2023Masato Sorida, Roberto BonasioGeneRuler 1 kb Plus DNA Ladder,Thermo Scientific,SM1331 BsaI-HFv2,NEB,R3733S BbsI-HF,NEB,R3539S AgeI-HF,NEB,R3552S BamHI-HF,NEB,R3136S EcoRI-HF,NEB,R3101L HindIII-HF,NEB,R3104S NcoI-HF,NEB,R3193S NotI-HF,NEB,R3189S SalI-HF,NEB,R3138S SpeI-HF,NEB,R3133S XbaI,NEB,R0145S XhoI,NEB,R0146S XmaI,NEB,R0180S KpnI-HF,NEB,R3142S NdeI,NEB,R0111S Smal,NEB,R0141S BbsI-HF,NEB,R3539M T4 DNA Ligase

(6) Differentiation block in acute myeloid leukemia regulated by intronic sequences of FTOiScienceAugust 18, 2023Francesco Camera, Isabel Romero-Camarero, Bradley H. Revell, Fabio M.R. Amaral, Oliver J. Sinclair, Fabrizio Simeoni, Daniel H. Wiseman, Lovorka Stojic, Tim C.P. SomervailleCas9,Integrated DNA Technologies,Cat#1081059 XbaI,NEB,Cat#R0145L SbfI,NEB,Cat#R3642L Age1,NEB,Cat#R3552S EcoR1-HF,NEB,Cat#R3101L DpnII,NEB,Cat#R0543S NlaIII,NEB,Cat#R0125S ,, Critical commercial assays,Critical

(7) A metabolic map of the DNA damage response identifies PRDX1 in the control of nuclear ROS scavenging and aspartate availabilityMolecular Systems BiologyJuly 11, 2023Amandine Moretton, Savvas Kourtis, Antoni Gañez Zapater, Chiara Calabrò, Maria Lorena Espinar Calvo, Frédéric Fontaine, Evangelia Darai, Etna Abad Cortel, Samuel Block, Laura Pascual‐Reguant, et al.Express,HY‐16397A TTFA,abcam,ab223880 Aspartic acid,Sigma‐Aldrich/ Merck,A1330000/ A7219 Carboplatin,MedChem Express,HY‐17393 AgeI‐HF,NEB,R3552S EcoRI‐HF,NEB,R3101S BsmBI,NEB,R0580L Lipofectamine 2000 transfection reagent,Thermo Fisher Scientific,11668019 Puromycin dihydrochloride

(8) Non-viral nitric oxide-based gene therapy improves perfusion and liposomal doxorubicin sonopermeation in neuroblastoma modelsTheranosticsJune 4, 2023Aditi Bellary, Chance Nowak, Isabella Iwanicki, Fernando Flores-Guzman, Lydia Wu, Jessica J. Kandel, Theodore W. Laetsch, Leonidas Bleris, Sonia L. Hernandez, Shashank R. Sirsiamplicon containing the iNOS transcript and the mKate2 expression vector was digested with AgeI-HF (NEB, #R3552S) and MfeI-HF (NEB, #R3589S). The iNOS transcript was cloned into the expression vector with T4 DNA Ligase

(9) Integrative identification of non-coding regulatory regions driving metastatic prostate cancerbioRxiv : the preprint server for biologyJune 2, 2023Woo BJ, Moussavi-Baygi R, Karner H, Karimzadeh M, Garcia K, Joshi T, Yin K, Navickas A, Gilbert LA, Wang B, et al.purified using 1.2:1 HighPrep PCR reagent. pLS-SceI backbone was then digested with AgeI-HF (NEB Cat. # R3552S) and SbfI (0.5U/uL) overnight, and then purified using 0.65:1 HighPrep PCR reagent. Linearized pLS-SceI

(10) Mutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activityJournal of Biological ChemistryJune 1, 2023Michael J. Hollander, Stacy A. Malaker, Nicholas M. Riley, Idalia Perez, Nayla M. Abney, Melissa A. Gray, Julia E. Maxson, Jennifer R. Cochran, Carolyn R. Bertozzi(New England Biolabs R3136S)/MfeI-HF (New England Biolabs R3589S) (gp130), MfeI-HF/AgeI-HF (New England Biolabs R3552S) (LIFR), AgeI-HF/PsiI-v2 (Thermo Fisher Scientific NC1704764) (OSMR), BsiWI-HF (Thermo Fisher Scientific

(11) Tailoring Magnetite-Nanoparticle-Based Nanocarriers for Gene Delivery: Exploiting CRISPRa Potential in Reducing ConditionsNanomaterials (Basel)May 31, 2023David Arango, Javier Cifuentes, Paola Ruiz Puentes, Tatiana Beltran, Amaury Bittar, Camila Ocasión, Carolina Muñoz-Camargo, Natasha I. Bloch, Luis H. Reyes, Juan C. Cruzthe thiolated tag (used to bind the nanobioconjugate to the linearized plasmid), AgeI-HF Enzyme (NEB-R3552S), EcoRI-HF Enzyme (NEB-R3101S), PvuI-HF Enzyme (NEB-R3150S), Q5 High-Fidelity 2X Mastermix (NEB-M0515),

(12) Overexpression of salusin‑α upregulates AdipoR2 and activates the PPARα/ApoA5/SREBP‑1c pathway to inhibit lipid synthesis in HepG2 cellsInternational Journal of Molecular MedicineApril 6, 2023Huan Zhang, Chao Yang, Songjiao Wang, Aohong Xu, Qian Zhang, Xiuqun Duan, Guofu Gong, Yuxue WangDNA, which was then ligated with the line vector of pLKO.1 that had been digested by AgeI (cat. no. R3552S; New England BioLabs, Inc.) and EcoRI (cat. no. R3101V; New England BioLabs, Inc.). The constructed

(13) The ion channel CALHM6 controls bacterial infection‐induced cellular cross‐talk at the immunological synapseThe EMBO JournalApril 3, 2023Sara Danielli, Zhongming Ma, Eirini Pantazi, Amrendra Kumar, Benjamin Demarco, Fabian A Fischer, Usha Paudel, Jillian Weissenrieder, Robert J Lee, Sebastian Joyce, et al.transcriptase 20,000",Takara Bio Europe SAS,639524 Collagenase type 2,Worthington‐Biochem,CLS‐2 Agel‐HF,NEB,R3552S HindIII‐HF,NEB,R3104S SalI‐HF,NEB,R3138S Assays and kits,Assays and kits,Assays and kits IL‐1 beta mouse uncoated ELISA Kit

(14) LncGMDS-AS1 promotes the tumorigenesis of colorectal cancer through HuR-STAT3/Wnt axisCell death & diseaseFebruary 27, 2023Ye D, Liu H, Zhao G, Chen A, Jiang Y, Hu Y, Liu D, Xie N, Liang W, Chen X, et al.Supplementary Table 5. The following reagents were used: NEBuffer 2 (B7002S, New England Biolabs), Age I (R3552S, NEB), EcoR I (R3101S, NEB), BamH I (FD-0054, Thermo Fisher), DNA ligation kit reagents (6022, TaKaRa),

(15) Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBDiScienceJanuary 20, 2023Chen Y, Prévost J, Ullah I, Romero H, Lisi V, Tolbert WD, Grover JR, Ding S, Gong SY, Beaudoin-Bussières G, et al.,New England Biolabs,Cat # R0603S BsiWI-HF,New England Biolabs,Cat # R3553S AgeI-HF,New England Biolabs,Cat # R3552S NheI-HF,New England Biolabs,Cat # R3131S Octet® Amine Reactive Second Generation (AR2G) biosensors,Sartorius,Cat

(16) Scd1 Deficiency in Early Embryos Affects Blastocyst ICM Formation through RPs-Mdm2-p53 PathwayInternational journal of molecular sciencesJanuary 16, 2023Niu H, Lei A, Tian H, Yao W, Liu Y, Li C, An X, Chen X, Zhang Z, Wu J, et al.pST1374-NLS-flag-linker-Cas9 plasmid (Addgene#44758, Cambridge, MA, USA) was used, which was digested with Age I (R3552S, NEB, Ipswich, MA, USA) at 37 °C overnight. After the plasmid linearization, RNA secure reagent was

(17) A Novel in Duck Myoblasts: The Transcription Factor Retinoid X Receptor Alpha (RXRA) Inhibits Lipid Accumulation by Promoting CD36 ExpressionInternational journal of molecular sciencesJanuary 7, 2023Pan Z, Chen X, Wu D, Li X, Gao W, Li G, Du G, Zhang C, Jin S, Geng Z.the full-length RXRA sequence, and upper and lower primers were designed, including the AgeI (NEB, R3552S) and EcoRi (NEB, R310T) restriction sites at the 5 ft and 3 ft ends, respectively. The primers (Supplementary

(18) Systematic discovery and functional dissection of enhancers needed for cancer cell fitness and proliferationCell ReportsNovember 8, 2022Poshen B. Chen, Patrick C. Fiaux, Kai Zhang, Bin Li, Naoki Kubo, Shan Jiang, Rong Hu, Emma Rooholfada, Sihan Wu, Mengchi Wang, et al.FD0454 FastAP,Thermo Fisher,Cat# EF0651 MboI,NEB,Cat# R0147S SalI-HF,NEB,Cat# R3138S AgeI-HF,NEB,Cat# R3552S Gibson Assembly Master Mix,NEB,Cat# E2611S Quick ligation kit,NEB,Cat# M2200S T4 PNK,NEB,Cat# M0201S Protein A Magnetic Beads

(19) Potent monoclonal antibodies neutralize Omicron sublineages and other SARS-CoV-2 variantsCell ReportsNovember 1, 2022Zhaochun Chen, Peng Zhang, Yumiko Matsuoka, Yaroslav Tsybovsky, Kamille West, Celia Santos, Lisa F. Boyd, Hanh Nguyen, Anna Pomerenke, Tyler Stephens, et al.,New England BioLabs,R3131S NotI,New England BioLabs,R3189S ApaI,New England BioLabs,R0114S AgeI,New England BioLabs,R3552S T4 DNA ligase,New England BioLabs,M0202M Bovine Serum Albumine (BSA),Sigma-Aldrich,3059 Tween 20,Sigma-Aldrich,93773 DMSO,Sigma-Aldrich,D2650 ,, Critical

(20) Effects of CRISPR/Cas9-mediated stearoyl-Coenzyme A desaturase 1 knockout on mouse embryo development and lipid synthesisPeerJSeptember 14, 2022Huibin Tian, Huimin Niu, Jun Luo, Weiwei Yao, Wenchang Gao, Yang Wen, Min Cheng, Anmin Lei, Jinlian Huaanalysis of sgRNA activity pST1374-NLS-flag-linker-Cas9 vector (Addgene#44758) was linearized with Age I (R3552S, NEB) and used for Cas9 mRNA in vitro transcription using the T7 Ultra Kit (AM1345; Invitrogen, Waltham,

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AgeI-HF

AgeI-HF from New England Biolabs

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New England Biolabs

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AgeI-HF from New England Biolabs

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