MagneGST Glutathione Particles from Promega

Product Specs
ItemMagneGST Glutathione Particles
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV8612
Quantity20ml
Product TypeResins, Magnetic Beads
ModeAffinity Purification
Applicationspurification

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Sequence-directed concentration of G protein-coupled receptors in COPII vesiclesiScienceOctober 20, 2023Xin Xu, Nevin A. Lambert, Guangyu WuProLong gold antifade mountant with DAPI,Invitrogen,Cat# P36931 MagneGST™ glutathione particles,Promega,Cat# V8611 Nano-Glo HiBiT Extracellular Detection System,Promega,Cat# N2420 Dulbecco’s modified Eagles medium,HyClone,Cat#

(2) The Troyer syndrome protein spartin mediates selective autophagy of lipid dropletsNature cell biologyAugust 1, 2023Chung J, Park J, Lai ZW, Lambert TJ, Richards RC, Zhang J, Walther TC, Farese RV Jr.and cOmplete Protease Inhibitor Cocktail tablet, EDTA-free (Millipore-Sigma)) and mixed with 10 μl of MagneGST Glutathione Particles (Promega) for 1.5 h at 4 °C. Meanwhile, the HEK293T cell lysates transiently expressing mScarlet-I-tagged

(3) GATS tag system is compatible with biotin labelling methods for protein analysisScientific ReportsJune 23, 2023Kohdai Yamada, Fumiya Soga, Soh Tokunaga, Hikaru Nagaoka, Tatsuhiko Ozawa, Hiroyuki Kishi, Eizo Takashima, Tatsuya Sawasakisynthesized using a wheat germ cell-free reaction. The protein synthetic solution was mixed with 30 µL of MagneGST™ Glutathione Particles (Promega) previously equilibrated with PBS and rotated at 4 °C for 1 h. After the flow-through solvent

(4) Protocol for analyzing intact mRNA poly(A) tail length using nanopore direct RNA sequencingSTAR ProtocolsJune 1, 2023Koichi Ogami, Yuka Oishi, Shin-ichi HoshinoGlutathione Sepharose 4B,Cytiva,17-0756-01 Glutathione,FujiFilm,071-02014 Amicon Ultra-0.5 Ultracel 30 kDa,Merck Millipore,UFC503008 MagneGST Glutathione Particles,Promega,V8611 Yeast tRNA (10 mg/mL),Thermo Fisher Scientific,AM7119 "Turbo3C protease, (HRV3C Protease)",Nacalai

(5) p53 regulates the effects of DAPT on Rac1 activation and migration of non-small-cell lung cancer cellsHeliyonMarch 2, 2023Lei Liu, Hong Xu, Yue Shi, Jie Cui, Jinxia Wu, Shibao LiGAPDH. 2.7 GST-pulldown assay The GST-CRIB was purified from BL21 bacteria and isolated by incubation with MagneGST glutathione particles (Promega, Madison, WI) for 30 min at 4 °C. Then, the particles can bind to active Rac1 (Rac1-GTP) or

(6) Borrelia burgdorferi DnaA and the Nucleoid-Associated Protein EbfC Coordinate Expression of the dnaX-ebfC OperonJournal of BacteriologyJanuary 26, 2023Andrew C. Krusenstjerna, Timothy C. Saylor, William K. Arnold, Jamila S. Tucker, Brian Stevensonwith 1 M urea, 0.5 M urea, and no urea. The clarified soluble protein suspension was purified using MagneGST glutathione particles (Promega) according to manufacturer recommendations. Recombinant GST was purified using the same MagneGST

(7) A structural basis for the diverse linkage specificities within the ZUFSP deubiquitinase familyNature CommunicationsDecember 1, 2022Thomas Hermanns, Christian Pichlo, Ulrich Baumann, Kay Hofmannone representative gel is shown. Ubiquitin-binding assay A total of 10 µl glutathione particles (MagneGST™ Glutathione Particles, Promega) were saturated with 6His-GST tagged Mug105 or Mug105ZUP1-NT in 200 µl binding buffer (20 mM

(8) Structural basis for the unique multifaceted interaction of DPPA3 with the UHRF1 PHD fingerNucleic Acids ResearchNovember 28, 2022Keiichi Hata, Naohiro Kobayashi, Keita Sugimura, Weihua Qin, Deis Haxholli, Yoshie Chiba, Sae Yoshimi, Gosuke Hayashi, Hiroki Onoda, Takahisa Ikegami, et al.Npro. The mDPPA361-150 was further puriﬁed using HiTrap SP HP and HiLoad 16/60 Superdex 30 (Cytiva). MagneGST™ Glutathione Particles (Promega) were used for the assay. The truncated GST-mUHRF1 (10 μg) were immobilized on the beads (10

(9) Phosphoproteomic of the acetylcholine pathway enables discovery of the PKC-β-PIX-Rac1-PAK cascade as a stimulatory signal for aversive learningMolecular psychiatryAugust 1, 2022Yamahashi Y, Lin YH, Mouri A, Iwanaga S, Kawashima K, Tokumoto Y, Watanabe Y, Faruk MO, Zhang X, Tsuboi D, et al.100 μM IPTG and induced for 16 h at 25 °C. GST-RacG15A was purified by the batch-binding method using MagneGST glutathione particles (Promega, Madison, WI, USA). GST-RacG15A-bound beads were washed with lysis buffer twice and wash buffer

(10) KPNB1 modulates the Machado-Joseph disease protein ataxin-3 through activation of the mitochondrial protease CLPPCellular and molecular life sciences : CMLSJuly 6, 2022Abeditashi M, Weber JJ, Pereira Sena P, Velic A, Kalimeri M, Incebacak Eltemur RD, Schmidt J, Hübener-Schmid J, Hauser S, Macek B, et al.protein assay. 500 µg prokaryotic protein extract containing GST-15Q or GST-77Q ataxin-3 were immobilized on MagneGST Glutathione Particles (Promega, Walldorf, Germany) for 1 h at 4 °C followed by four times washing with GST binding buffer

(11) Z-DNA is remodelled by ZBTB43 in prospermatogonia to safeguard the germline genome and epigenomeNature Cell BiologyJuly 4, 2022Yingying Meng et al.reaction MIRA proteins, 1 µg of GST-MBD2b and 1 µg His-MBD3L1, and the MagneGST beads (Promega, cat. no. V8611) were pre-blocked with 500 ng unmethylated E.Coli genomic DNA in 1× MIRA binding buffer (10 mM Tris–HCl

(12) Human C1orf27 protein interacts with α(2A)-adrenergic receptor and regulates its anterograde transportThe Journal of biological chemistryJune 1, 2022Xu X, Wu G.and dynabeads protein G were from Thermo Fisher Scientific. UK14304 was obtained from Sigma-Aldrich. MagneGST glutathione particles were from Promega. All other materials were obtained as described elsewhere (49, 61). Plasmids and

(13) Microarray screening reveals two non-conventional SUMO-binding modules linked to DNA repair by non-homologous end-joiningNucleic acids researchMay 6, 2022Cabello-Lobato MJ, Jenner M, Cisneros-Aguirre M, Brüninghoff K, Sandy Z, da Costa IC, Jowitt TA, Loch CM, Jackson SP, Wu Q, et al.using a Hiload 16/600 Superdex 75 prep grade column (GE Healthcare). GST-4×SUMO2 was purified using MagneGST Glutathione particles (Promega) as previously described (30). XLF-6×His was purified using a HP His-trap column followed by

(14) Phytochromes A and B Mediate Light Stabilization of BIN2 to Regulate Brassinosteroid Signaling and Photomorphogenesis in ArabidopsisFrontiers in Plant ScienceMarch 30, 2022Jiachen Zhao et al.E. coli (Rosetta). For GST pull-down assays, MagneGST™ Glutathione Particles (Promega, United States, V8611) were used. Prey proteins were detected with anti-His (GenScript, China, A00186). Split-Luciferase

(15) The osa‐miR164 target OsCUC1 functions redundantly with OsCUC3 in controlling rice meristem/organ boundary specificationNew PhytologistFebruary 1, 2021Jun Wang, Jinlin Bao, Beibei Zhou, Min Li, Xizhi Li, Jian Jinmanufacturer’s instructions. The recombinant GST‐OsCUC1 fusion protein was immobilized on GST‐Binding Resin (MagneGST Glutathione Particles, Promega) and incubated with MBP‐OsCUC3 for 4 h at 4°C. After incubation, the beads were washed five

(16) The apple DNA-binding one zinc-finger protein MdDof54 promotes drought resistanceHorticulture ResearchDecember 1, 2020Pengxiang Chen et al.was also purified to obtain GST proteins. One hundred microliters of MagneGST Glutathione Particles (V8611, Promega, USA) was washed with MagneGST Binding/Wash Buffer and resuspended in the buffer to a final

(17) Quantifying the heterogeneity of macromolecular machines by mass photometryNature CommunicationsDecember 1, 2020Adar Sonn-Segev et al.purification buffer. Proteasomes were affinity purified with the bait protein GST-Ubl and magnetic beads (MagneGST™ Glutathione Particles, Promega) and eluted with purification buffer containing 25 mM reduced L-glutathione. Proteasome samples

(18) LncRNA SNHG11 facilitates tumor metastasis by interacting with and stabilizing HIF-1Î±OncogeneNovember 12, 2020Linguo Xu, Lin Huan, Tianan Guo, Yangjun Wu, Yanfang Liu, Qifeng Wang, Shenglin Huang, Ye Xu, Linhui Liang, Xianghuo Heexperimental procedure was similar to that of the RIP protocol with minor modifications. Briefly, 50 μl of magneGST glutathione particles (Promega, WI, USA) was added to the cell lysate. Twenty percent of the beads were lysed in TRIzol, and

(19) PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasionScience advancesNovember 1, 2020Yu K, Tian H, Deng H.concentrations were measured with Enhanced BCA Protein Assay Kit (Beyotime, P0010). GST pull-down assay The MagneGST glutathione particles (Promega, V861A) were washed three times with PBS for particle equilibration by DynaMag-2 Magnet (Life Technologies

(20) DNA binding activity of the proximal C-terminal domain of rat DNA topoisomerase IIβ is involved in ICRF-193-induced closed-clamp formationPLOS ONESeptember 22, 2020Shinji Kawano, Kunpei Fujimoto, Kazushi Yasuda, Shogo Ikedaperformed as previously described [1]. FLAG-tagged topo IIβ WT (100 ng) and GST-histone H1.0 immobilized on MagneGST Glutathione Particles (Promega) were mixed in 30 μL of PD buffer containing 50 mM Tris-HCl (pH 8.0), 120 mM KCl, 10 mM MgCl2,

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MagneGST Glutathione Particles from Promega

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MagneGST Glutathione Particles from Promega

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