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Glutathione-Agarose
Glutathione-Agarose from Santa Cruz Biotechnology, Inc.
Be the first to write a review!
Citations:
(16)
Supplier Page
Supplier Page from
Santa Cruz Biotechnology, Inc. for
Glutathione-Agarose
Description
provided as 0.5 ml agarose/2.0 ml; 100 reactions
pre-blocked with BSA to reduce non-specific immunoglobulin binding
suitable for use at 20 µl per immunoprecipitation reaction
specific binding to GST fusion proteins
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Glutathione-Agarose
Company
Santa Cruz Biotechnology, Inc.
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
sc-2009
Quantity
2 ml
Matrix
Agarose
Type
Agarose
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Santa Cruz Biotechnology, Inc.
10410 Finnell Street
Dallas
,
Texas
75220
United States
Phone
: 1-800-457-3801
831-457-3801
Company Profile
Website:
http://www.scbt.com/
Citations (16)
(1)
Critical role of mitogen-inducible gene 6 in restraining endothelial cell permeability to maintain vascular homeostasis
Journal of cell communication and signaling
March 1, 2023
Xing L, Huang G, Chen R, Huang L, Liu J, Ren X, Wang S, Kuang H, Kumar A, Kim JK,
et al
.
protein or 5 μg of each GST-
VEGFR2
fusion proteins were added to 30 μl of glutathione agarose beads (
sc-2009
,
Santa Cruz
) and incubated at 4°C for 2 h. The protein bound-beads were then washed three times with
(2)
The common YAP activation mediates corneal epithelial regeneration and repair with different-sized wounds
NPJ Regenerative medicine
March 26, 2021
Li Y, Ge L, Chen X, Mao Y, Gu X, Ren B, Zeng Y, Chen M, Chen S, Liu J,
et al
.
loading and total Rho controls. The
cell lysis
was incubated with glutathione agarose beads (
Santa Cruz
,
sc-2009
) pre-coated with the Rho-GTP binding domain of human Rhotekin protein tagged with GST (GST-RBD protein;
(3)
An ATM-Chk2-INCENP pathway activates the abscission checkpoint
The Journal of cell biology
February 1, 2021
Petsalaki E, Zachos G.
To prepare GST fusion protein bound to glutathione agarose beads, 150 µl glutathione agarose slurry (
sc-2009
;
Santa Cruz Biotechnology
) was added for every 3 ml crude extract and mixed for 1 h at 4°C using a spiramix;
(4)
Loss of function Cbl-c mutations in solid tumors
PLOS ONE
July 1, 2019
Silvano Rakeem Daniels, Mariya Liyasova, Stephen C. Kales, Marion M. Nau, Philip E. Ryan, Jeffrey E. Green, Stanley Lipkowitz
Cruz Biotechnology, Dallas, TX) were used for
immunoprecipitation
of EGFR. Glutathione-agarose beads (
sc-2009
;
Santa Cruz Biotechnology
, Dallas, TX) were used for the
pull-down
of GST-tagged mutant Cbl-c. Horseradish
(5)
Cbl interacts with multiple E2s in vitro and in cells
PloS one
January 1, 2019
Liyasova MS, Ma K, Voeller D, Ryan PE, Chen J, Klevit RE, Lipkowitz S.
EDTA, 1%
Triton X-100
, 10% glycerol) supplemented with 30 μl of glutathione agarose beads (50% slurry,
sc-2009
,
Santa Cruz Biotechnology
). Syk was
immunoprecipitated
from 15 μl aliquot with anti-Syk antibody and
(6)
Ubiquitin-specific protease 4 promotes hepatocellular carcinoma progression via cyclophilin A stabilization and deubiquitination
Cell Death & Disease
February 1, 2018
Tianyi Li, Bin Yan, Yang Ma, Junyong Weng, Shouwen Yang, Nan Zhao, Xiaoliang Wang, Xing Sun
GST or GST-CypA fusion proteins (Sangon Biotech, Shanghai, China) and 20 μL GST-beads (
Santa Cruz
,
sc-2009
) were incubated with cell lysates for 6 h at 4 °C. The beads were then collected and washed three times
(7)
Sphingosine kinase 2 activates autophagy and protects neurons against ischemic injury through interaction with Bcl-2 via its putative BH3 domain
Cell Death & Disease
July 1, 2017
Dan-Dan Song, Tong-Tong Zhang, Jia-Li Chen, Yun-Fei Xia, Zheng-Hong Qin, Christian Waeber, Rui Sheng
against HA,
Bcl-2
(
Santa Cruz
sc-492
) or Beclin-1. GST
pull-down
Glutathione-agarose
(
Santa Cruz
sc-2009
) beads were washed with 1%
Triton X-100
PBS, and were incubated with GST (Flarebio, Wuhan, China, CSB-RP101744Ba)
(8)
Targeting sarcoma tumor-initiating cells through differentiation therapy
Stem Cell Research
May 1, 2017
Dan Han, Veronica Rodriguez-Bravo, Elizabeth Charytonowicz, Elizabeth Demicco, Josep Domingo-Domenech, Robert G. Maki, Carlos Cordon-Cardo
sections. Primary antibodies for HLA class I W6/32 (
Abcam
,
ab23755
),
CD44
(
BD
,
550392
), Sox-9 (
Santa Cruz
,
sc2009
), and actin (Sigma, AC-15) were used. 2.4
Tumor
Xenograft Formation and Tumorigenic Capacity
Assay
All
(9)
The TRAIL receptor agonist drozitumab targets basal B triple-negative breast cancer cells that express vimentin and Axl
Breast cancer research and treatment
January 1, 2016
Dine JL, O'Sullivan CC, Voeller D, Greer YE, Chavez KJ, Conway CM, Sinclair S, Stone B, Amiri-Kordestani L, Merchant AS,
et al
.
described above, and DR5 precipitated by adding agarose agarose-glutathione (agarose-GSH) beads (Cat #
sc-2009
,
Santa Cruz Biotechnology
, Dallas, TX) to the lysate. For the cells that had been incubated without
(10)
IndexFS: Scaling File System Metadata Performance with Stateless Caching and Bulk Insertion
SC14: International Conference for High Performance Computing, Networking, Storage and Analysis
November 1, 2014
Kai Ren, Qing Zheng, Swapnil Patil, Garth Gibson
scale. In Proceedings of the Conference on High Performance Computing Networking, Storage and Analysis (
SC), 2009
. [55] [30] A. Leung, I. Adams, and E. L. Miller. Magellan: A searchable metadata architecture for
(11)
ROCK1 deficiency enhances protective effects of antioxidants against apoptosis and cell detachment
PloS one
January 1, 2014
Surma M, Handy C, Chang J, Kapur R, Wei L, Shi J.
beads (#
17–283
,
Millipore
). Cell lysates (800 µg) were pre-cleared with glutathione agarose beads (
SC-2009
,
Santa Cruz
)) at 4°C for 10 min. The supernatant was then incubated with PAK-1 PBD-agarose beads at
(12)
ROCK1 deficiency enhances protective effects of antioxidants against apoptosis and cell detachment
PloS one
January 1, 2014
Surma M, Handy C, Chang J, Kapur R, Wei L, Shi J.
(13)
Rapid and Sensitive Lentivirus Vector-Based Conditional Gene Expression Assay to Monitor and Quantify Cell Fusion Activity
PLoS ONE
June 3, 2010
Manuel A. F. V. Gonçalves, Josephine M. Janssen, Maarten Holkers, Antoine A. F. de Vries
a 3-hour
incubation
with
horseradish
peroxidase-conjugated goat-anti-rabbit IgG secondary antibody (
SC-2009
;
Santa Cruz Biotechnology
) diluted 1∶5000 in blocking solution. Detection of α/β-tubulin proteins by
(14)
Rapid and Sensitive Lentivirus Vector-Based Conditional Gene Expression Assay to Monitor and Quantify Cell Fusion Activity
PLoS ONE
June 3, 2010
Manuel A. F. V. Gonçalves, Josephine M. Janssen, Maarten Holkers, Antoine A. F. de Vries
(15)
Differentiation of Human Embryonic Stem Cells to a Parathyroid-Like Phenotype
Stem Cells and Development
September 1, 2009
Eve L. Bingham, Shih-Ping Cheng, Kathleen M. Woods Ignatoski, Gerard M. Doherty
Santa Cruz Biotechnology, Inc
., Santa Cruz, CA), anti-
CaSR
(Cat#
sc-47741
;
Santa Cruz
Biotech), and anti-Sox17 (Cat#
sc-2009
;
Santa Cruz
Biotech) were incubated with the cells overnight at 4°C. Cells were then exposed to appropriate
(16)
Wnt-3a and Dickkopf-1 stimulate neurite outgrowth in Ewing tumor cells via a Frizzled3- and c-Jun N-terminal kinase-dependent mechanism
Molecular and cellular biology
April 1, 2008
Endo Y, Beauchamp E, Woods D, Taylor WG, Toretsky JA, Uren A, Rubin JS.
overnight at 4°C in a rotary shaker, followed by the addition of glutathione agarose beads (catalog no.
sc2009
;
Santa Cruz Biotechnology
) and
incubation
for 1 or 2 h at 4°C. The beads were spun down and washed three
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Glutathione-Agarose from Santa Cruz Biotechnology, Inc.
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Pricing Info
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