Since its introduction in 2017, the SpectraMax® i3x Multi-Mode Microplate Reader from Molecular Devices has elevated life science research and drug development by boosting throughput and delivering reliable absorbance, fluorescence, and luminescence measurements across a range of biological samples. Upgrades covering western blot, cell imaging, fast kinetics, and other additional detection modes and modules ensure utility no matter where your research takes you.
Though Molecular Devices’ microplate readers are designed to provide consistent performance for many years, best practices and strict regulations require microplate reader performance to be validated at regular intervals. The latest upgrade for the SpectraMax® i3x Multi-Mode Microplate Reader makes this required validation a breeze and guarantees local quality and GLP regulations for lab equipment validation are easily met at an impressive linearity range up to 3.0 OD. Validation can be accomplished using a SpectraTest® ABS2 validation plate and the extended photometric linearity validation protocol, which confirms more reliable measurements at higher absorbance levels—proof that is crucial in various research and quality control applications.
The protocol is underpinned by the SpectraTest ABS2 Absorbance Validation Plate. Each filter is individually tested across eight channels. Columns 1 and 2 of the ABS2 plate test the optical alignment and dynamic range of the light beam to make sure that the light beam goes through your sample and does not touch any well walls. Columns 3–6 contain filters with increasing optical density to test the photometric accuracy, precision and linearity. Columns 7–10 contain filters to make sure that only the light of the correct wavelength is leaving the monochromators. Finally, wavelength accuracy and precision are measured at positions 11 and 12.
ABS2 Absorbance Validation Plate simplifies the certificate entry process to reduce human error and expand the testing capabilities to include more wavelengths. Calibration of the plate’s filter standards is accomplished through the use of an instrument calibrated with primary standards from the National Institute of Standards and Technology.
IQ/OQ/PM Services and SoftMax Pro GxP Software from Molecular Devices—the industry leader in bioanalytical measurement systems and software—qualifies and preserves documentation in a compliant format that can be accessed remotely.
In fact, use of the company’s extensive suite of validation tools has been shown to reduce the cost and time of validation by 50%. By making validation faster and less expensive, even at the edge of usable optical range, the new protocol ensures SpectraMax® i3x Multi-Mode Microplate grows with your research needs and accelerates otherwise time-consuming documentation and validation.