Multiplex qPCR assays boost efficiency and reduce costs for life science research and diagnostics labs, but clean resolution of individual fluorescence signals is not always easy to attain. Crosstalk can result in false-positive signals or artificially inflated signals, leading to inaccurate quantification of target molecules—ultimately limiting utility of multiplex assays and delaying actionable results.
But the qTOWERiris, a new offering from Analytik Jena, addresses the crosstalk challenge while also boasting new features such as an expanded wavelength range that increase flexibility and multiplexing capabilities.
The qTOWERiris employs a unique, multicolor LED light source and matching filter modules to deliver optimal resolution of individual color channels. In fact, by exciting each well individually and detecting from the same angle, qTOWERiris provides the most homogeneous amplification plots without edge effects, significantly outperforming traditional camera optics.
It also leads comparable qPCR modules in multiplex capabilities. The qTOWERiris allows for six targets simultaneously, with no passive reference necessary. An extended wavelength range, up to the UVA spectrum, makes qTOWERiris compatible with all available dyes as well as those yet to be launched. Recalibration is not required for dye changes but can be performed at any time. New software settings are available to selectively amplify weaker signals.
A high ramping rate means target temperature is reached precisely and quickly, reducing time to results and, most importantly, preventing false amplification. Heat conduction is uniform for all 96 wells thanks to an innovative gold-coated silver block that is twice as conductive as traditional aluminum coatings. In fact, qTOWERiris has demonstrated unparalleled temperature homogeneity across the entire block, with documented temperature deviation of just +/- 0.15ºC—well below the market standard of +/-0.4 ºC.
The qTOWERiris also provides flexibility. Available in three variants, qTOWERiris gives users the freedom to choose consumables, reagents, and assays. Up to four devices can be operated with a single PC. All types of microplates—skirted, non-skirted, and half-skirted—can be used with qTOWERiris. Software provides a comprehensive PDF report and remains license-free. All raw data are freely accessible or processed as interpolated curves.
Introduced mere months ago, feedback has been overwhelmingly positive. One lab lead praised the qTOWERiris for being fast, easy to use, and “great” for multiplexing. “A bonus is the gain settings to enhance the signal depending on the dye,” they said. “This saves us money in assay development, as well as the freedom to choose the plastic. qTOWERiris makes our work easier in every respect—and it’s super quiet too.”
Analytik Jena also offers direct application support and a 10-year warranty on the patented fiber-optical system, ensuring your lab has the most efficient and reliable qPCR capabilities for years to come.