Beta-galactosidase assay kits are available from several suppliers to determine the enzymatic activity of beta-galactosidase. This enzyme hydrolyzes beta-galactosides into monosaccharides by breaking a glycosidic bond, thus playing an important role in the metabolism of carbohydrates for energy generation. In beta-galactosidase assays, substrates such as ONPG (o-nitrophenyl-beta-D-galactopyranoside) are hydrolyzed by the enzyme to produce colored measurable byproducts that can be observed by spectrophotometry. Beta-galactosidase assays are commonly used in molecular biology experiments. Visit the beta-galactosidase assay kit suppliers for more details, including kit components.
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- Cell-Based Assay, Immunohistochemistry
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