7-AAD (7-Aminoactinomycin D) is a fluorescent dye that has a strong affinity for DNA, particularly GC-rich regions. It intercalates with double-stranded DNA to produce a red-colored fluorescence. It has an absorption maximum at 546 nm and an emission maximum at around 647 nm. It is fairly compatible with many blue and green fluorophores. 7-AAD is cell membrane impermeable and can only stain cellular DNA if the membrane is permeabilized. It is used in cell viability staining, fluorescence microscopy, and flow cytometry.
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- 7-Aminoactinomycin D (7-AAD)
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