Fig 1: Abundance of FNDC3A (A), FNDC3B (B), FNDC4 (C), FNDC5 (E), ITGAV (G), ITGB1 (F) and ADGRF5 (D) mRNA in bovine subcutaneous adipose tissue during peripartum period. Tissues were obtained 4 weeks before parturition (4WAP) and 1 (1WPP) and 16 weeks post-partum (16 WPP) and mRNA measured by real-time RT-qPCR. Data are expressed as mean ± SEM for each group (n = 8 in LNEB group and n = 13 in HNEB group) and analysed by a nonparametric analysis of variance with repeated measures (Friedman test). Groups with different letters are significantly different (p < 0.05). * indicates a significant difference (p < 0.05).
Fig 2: Plasma concentrations of FNDC proteins at 4 WAP, 1 WPP and 16 WPP in HNEB and LNEB animals. The concentrations of FNDC3A (A), FNDC3B (B), FNDC4 (C) and FNDC5 (D) in plasma were determined by ELISA assays. Data are expressed as mean ± SEM for each group of animals (HNEB and LNEB) at each stage of the peripartum period (4WAP, 1 and 16 WPP, n = 8) and analysed by a nonparametric analysis of variance with repeated measures (Friedman test). Groups with different letters were significantly different (p-value < 0.05).
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