Description
The Eagle Biosciences Anti-M2 ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of IgG antibodies to the mitochondrial Antigens M2. The antibodies of the standards, controls, and diluted patient samples react with to the native mitochondrial Antigens M2 immobilized on the solid phase of microtiter plates. The use of highly purified native mitochondrial Antigens M2 guarantees the specific binding of M2 antibodies of the specimen under investigation. Following an incubation period of 30 min at room temperature (RT), unbound serum components are removed by a wash step. The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP) within the incubation period of 30 min at RT. Excessive conjugate is separated from the solid-phase immune complexes by the following wash step. HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethyl¬benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution (HCl) into the wells after 30 min at RT turning the solution from blue to yellow.The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the antibody concentrations of the standards (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve