Fig 1: Phosphorylation of p53 and modulation of its downstream genes.(A) Increased p53 phosphorylation (ser 15) was induced by tIRI (*p < 0.0001) and was inhibited by FDP treatment (#p < 0.0001). Real-time PCR was used to measure the relative mRNA expression of (B) PUMA and (C) survivin. PUMA mRNA levels were increased during tIRI (*p < 0.05), whereas survivin mRNA was decreased (*p < 0.05). FDP treatment normalized the relative mRNA expression of PUMA (#p < 0.05) and survivin (#p < 0.05). Data analysis was performed using one-way analysis of variance (ANOVA) followed by the Holm-Sidak multiple comparisons test. Data are presented as the mean ± SD (n = 6). The significance of the data is indicated with * compared with the sham group and # compared with the tIRI group.
Fig 2: Immunoexpression of p53 and TIGAR in paraffin-embedded ipsilateral testicular tissue.(A and D) The sham group showing very weak immunostaining of the p53 and TIGAR antibodies, respectively, in the seminiferous tubules layers of the rat testes. (B and E) Examples of p53 and TIGAR immunostaining showing a notable increase in their immunoexpression after tIRI. (C and F) FDP treatment of tIRI-subjected rats showed a decrease in the p53 and TIGAR immunolabeling intensity in the testes compared with the tIRI group. Magnification = 40×.
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