Fig 1: Effect of the Eae39r2 sub-congenic fragment on Tbx5 (a) and Tbx3 (b) mRNA levels in the joints, Tbx3 mRNA levels in spleen (c) and purified splenic B lymphocytes (d). Values are presented as mean of 2^-??Ct and bars represent standard error of the mean (SEM). Statistical analyses were performed using two-way Student’s t test; *p < 0.05, **p < 0.01
Fig 2: Quantitative analysis of active TBX3 in nuclear lysates of CD19+ B cells from B10.RIII mice. a Comparison of active TBX3 levels in CD19+ B cells isolated from naïve B10.RIII mice (n = 4), with and without in vitro stimulation with a fixed concentration (10 µg/ml) of anti-IgM antibody for 48 h. b, c Collagen-induced arthritis (CIA) development and corresponding TBX3 activity in B10.RIII mice whereby four mice were killed at indicated time points after collagen type II (CII) immunization and levels of active TBX3 were determined with and without ex vivo re-stimulation with fixed concentration (10 µg/ml) of anti-IgM antibody for 48 h. Statistical analyses were performed using the Mann-Whitney U test; *significant (p < 0.05) compared to day 0 unstimulated samples; #significant compared to day 7 unstimulated samples; §significant compared to respective unstimulated samples
Fig 3: Kinetics of the expression of the TBX3 protein during development of collagen-induced arthritis (CIA) in the B10.RIII mouse strain. Levels of TBX3 in serum from CIA-immunized mice (a); comparison of serum-TBX3 levels between naïve mice (n = 4), immunized, non-arthritic mice (n = 14) and arthritic mice (n = 6) (b). Statistical analyses were performed using the Mann-Whitney U test; **p < 0.01
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