Fig 1: The effects of T1D on sarcomeric protein phosphorylation. (A,B) Examples of gel electrophoresis of NTF and myosin extracted from control (C) and diabetic (6 weeks of T1D) groups. TnT, troponin T; Tpm, tropomyosin; TnI, troponin I; cMyBP-C, cardiac myosin binding protein-C; ELC, myosin essential light chain; RLC, myosin regulatory light chain. Thermo Scientific PageRuler™ Unstained Protein Ladder was used as molecular weight markers for protein. (C) Phosphorylation levels of TnT, Tpm, and TnI. (D) Phosphorylation levels of cMyBP-C and RLC. Phosphorylation is expressed as the ratio of the intensities of protein bands stained with Pro-Q Diamond and SYPRO Ruby. LV, left ventricular free wall; IS, interventricular septum; RV, right ventricular free wall. The number of hearts is shown in parentheses above the first set of bars. Data are median (interquartile range). * p < 0.05, Kruskal–Wallis test.
Supplier Page from MyBioSource.com for Rat Troponin T, cardiac muscle ELISA Kit