MojoSort(TM) Mouse CD4 T Cell Isolation Kit 480005 from BioLegend

Product Specs
ItemMojoSort(TM) Mouse CD4 T Cell Isolation Kit
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number480005
Quantity10 tests
ApplicationsCell Sep - Neg
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
Detection TargetCD4
Cell Type (standardized)CD4+ T Cell
Conjugate/Tag/LabelMOJO

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Plasmacytoid dendritic cells contribute to vascular endothelial dysfunction in type 2 diabetesFrontiers in Cardiovascular MedicineNovember 29, 2023K. Alluri, B. Srinivas, S. Belmadani, K. Matrouguiand db/db mice using a CD4 T cell isolation kit from Biolegend as per manufacturer protocol (cat # 480006, San Diego, CA, United States) and cultured in RPMI 1640 with 1% penicillin-streptomycin. 1% Glutamax

(2) Noninvasive ultrasound stimulation to treat myocarditis through splenic neuro-immune regulationJournal of NeuroinflammationApril 17, 2023Tianshu Liu, Yanan Fu, Jiawei Shi, Shukun He, Dandan Chen, Wenqu Li, Yihan Chen, Li Zhang, Qing Lv, Yali Yang, et al.into single-cell suspensions. CD4+ T cell or CD11b+ macrophage was selected using an isolation kit (#480005 and #480109, Biolegend, USA). Total RNA was extracted using TRIzol Reagent according to the manufacturer’s

(3) Characterization of proteogenomic signatures of differentiation of CD4+ T cell subsetsDNA ResearchFebruary 1, 2023Toshio Kanno, Ryo Konno, Keisuke Miyako, Takahiro Nakajima, Satoru Yokoyama, Shigemi Sasamoto, Hikari K Asou, Junichiro Ohzeki, Yusuke Kawashima, Yoshinori Hasegawa, et al.were obtained by the negative selection using the Mojo Sort Mouse CD4 T Cell Isolation Kit (Biolegend #480006) and positive selection using CD62L MicroBeads, mouse (Miltenyi Biotec #130-049-701). Naïve CD4+ T cells

(4) A tick C1q protein alters infectivity of the Lyme disease agent by modulating interferon γCell reportsNovember 22, 2022Tang X, Arora G, Matias J, Hart T, Cui Y, Fikrig E.were isolated from splenocytes with MojoSort™ mouse CD4 and CD8 T cell isolation kits (BioLegend, #480005 and #480007). The purity of the isolated cells was evaluated by flow cytometry with the antibodies of

(5) Distinctive populations of CD4+T cells associated with vaccine efficacyiScienceSeptember 16, 2022Therese Woodring, Colin N. Dewey, Lucas Dos Santos Dias, Xin He, Hannah E. Dobson, Marcel Wüthrich, Bruce Klein1% FBS) and the CD4+T cells were enriched using MojoSort™ Mouse CD4 cell isolation (Biolegend, Cat#480006). The cells were stained and sorted as explained before in scRNAseq section as well. Both lungs and

(6) FOXP3 exon 2 controls T(reg) stability and autoimmunityScience immunologyJune 24, 2022Du J, Wang Q, Yang S, Chen S, Fu Y, Spath S, Domeier P, Hagin D, Anover-Sombke S, Haouili M, et al.Foxp3 FL (WT);DEREG or Foxp3 ΔE2;DEREG mice with MoJoSort mouse CD4 T cell isolation kit (BioLegend, 480006). Enriched CD4 T cells were then stained with anti-mouse CD4 and Tregs were sorted based upon eGFP expression

(7) SCD2-mediated cooperative activation of IRF3-IRF9 regulatory circuit controls type I interferon transcriptome in CD4(+) T cellsFrontiers in immunologyJanuary 1, 2022Kanno T, Miyako K, Nakajima T, Yokoyama S, Sasamoto S, Asou HK, Ohara O, Nakayama T, Endo Y.were obtained by the negative selection using the Mojo Sort Mouse CD4 T Cell Isolation Kit (Biolegend #480006) and positive selection using CD62L MicroBeads, mouse (Miltenyi Biotec #130-049-701). Naïve CD4+ T cells

(8) Gut epithelial IL-27 confers intestinal immunity through the induction of intraepithelial lymphocytesThe Journal of experimental medicineNovember 1, 2021Lin CH, Chen MC, Lin LL, Christian DA, Min B, Hunter CA, Lu LF.Next, CD4+ IELs were further isolated by using Mouse CD4 T Cell Negative Selection kit (BioLegend; 480006). Finally, isolated CD4+ IELs were stained with anti-CD8α-PE antibody, and CD8αα+CD4+ IELs were separated

(9) BFAR coordinates TGFβ signaling to modulate Th9-mediated cancer immunotherapyThe Journal of experimental medicineJuly 5, 2021Pei S, Huang M, Huang J, Zhu X, Wang H, Romano S, Deng X, Wang Y, Luo Y, Hao S, et al.

(10) Enhanced Susceptibility of Galectin-1 Deficient Mice to Experimental ColitisFrontiers in ImmunologyJune 28, 2021Raquel Fernandez-Perez, Mercedes Lopez-Santalla, Rebeca Sánchez-Domínguez, Omaira Alberquilla, Irene Gutiérrez-Cañas, Yasmina Juarranz, Juan A. Bueren, Marina I. Garin

(11) Pairwise Stimulations of Pathogen-Sensing Pathways Predict Immune Responses to Multi-adjuvant CombinationsCell SystemsNovember 1, 2020Surya Pandey, Adam Gruenbaum, Tamara Kanashova, Philipp Mertins, Philippe Cluzel, Nicolas Chevriercell suspension by positive selection using the MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend 480006). Cytokine quantifications. Cell culture supernatants were collected from (1) BMDC (100,000 cells/well)

(12) Blocking P2X7-Mediated Macrophage Polarization Overcomes Treatment Resistance in Lung CancerCancer immunology researchNovember 1, 2020Qin J, Zhang X, Tan B, Zhang S, Yin C, Xue Q, Zhang Z, Ren H, Chen J, Liu M, et al.

(13) PPARα Inhibition Overcomes Tumor-Derived Exosomal Lipid-Induced Dendritic Cell DysfunctionCell reportsOctober 20, 2020Yin X, Zeng W, Wu B, Wang L, Wang Z, Tian H, Wang L, Jiang Y, Clay R, Wei X, et al.cell Isolation Kit,Biolegend,Cat # 480035 MojoSort Mouse CD4 T cell Isolation Kit,Biolegend,Cat # 480005 10* Permeabilization buffer,eBioscience,Cat # 00-8333-56 Fix/Permeabilization buffer set,eBioscience,Cat

(14) Self-Assembled Multivalent Aptamer Nanoparticles with Potential CAR-like Characteristics Could Activate T Cells and Inhibit Melanoma GrowthMolecular therapy oncolyticsJune 26, 2020Bai C, Gao S, Hu S, Liu X, Li H, Dong J, Huang A, Zhu L, Zhou P, Li S, et al.with a nylon hair column (Polyscience, 21759-1) or a mouse CD4/CD8 T cell isolation kit (Biolegend, 480005/480007) and added to 1640 medium for subsequent experiments. T cells were then stained with CFDA-SE

(15) Host cytosolic RNA sensing pathway promotes T Lymphocyte-mediated mycobacterial killing in macrophagesPLoS pathogensMay 1, 2020Cheng Y, Kiene NJ, Tatarian A, Eix EF, Schorey JS.infection as described above). T cells were isolated using MojoSort™ Mouse CD4+ T Cell Isolation Kit (Cat. 480005, Biolegend) and MojoSort™ and mouse CD8+ T Cell Isolation Kit (Cat. 480007, Biolegend). Purified CD4+

(16) T cells instruct myeloid cells to produce inflammasome-independent IL-1β and cause autoimmunityNature immunologyJanuary 1, 2020Jain A, Irizarry-Caro RA, McDaniel MM, Chawla AS, Carroll KR, Overcast GR, Philip NH, Oberst A, Chervonsky AV, Katz JD, et al.506312 for detection), Naïve T cell Mojosort (Biolegend; 480040), total CD4+ T cell Mojosort (Biolegend; 480006), Zombie yellow (Biolegend; 423103), DAPI (Biolegend; 422801), IETD (R&D Systems; FMK007, 10μM), rGMCSF

(17) CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE ModelFrontiers in immunologyJanuary 1, 2020Wang N, Yi H, Fang L, Jin J, Ma Q, Shen Y, Li J, Liang S, Xiong J, Li Z, et al.from Cd226−/− or WT mice were negatively selected using the Mojosort™ Mouse CD4 T Cell Isolation kit (480006, Biolegend, San Diego, CA, USA). The purified CD4+ T cells (cell purity >95%) were cultured in RPMI

(18) Pyrrothiogatain acts as an inhibitor of GATA family proteins and inhibits Th2 cell differentiation in vitroScientific reportsNovember 22, 2019Nomura S, Takahashi H, Suzuki J, Kuwahara M, Yamashita M, Sawasaki T.

(19) Cross-Species Single-Cell Analysis of Pancreatic Ductal Adenocarcinoma Reveals Antigen-Presenting Cancer-Associated FibroblastsCancer discoveryAugust 1, 2019Elyada E, Bolisetty M, Laise P, Flynn WF, Courtois ET, Burkhart RA, Teinor JA, Belleau P, Biffi G, Lucito MS, et al.enriched for CD4+ T cells by negative selection using MojoSort mouse CD4 T cell isolation kit (Biolegend #480006). APC/CAF plates were washed twice, 50,000 CD4+ T cells were co-plated in 10% FBS/DMEM in each well,

(20) Uterine B Cells Exhibit Regulatory Properties During the Peri-Implantation Stage of Murine PregnancyFrontiers in immunologyJanuary 1, 2019Guzman-Genuino RM, Eldi P, Garcia-Valtanen P, Hayball JD, Diener KR.

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(1)

1/31/2020CD4 Enrichment of Cultured Splenocytes Using MojoSort Troy TrevinoThe MojoSort Mouse CD4 T cell isolation kit works very well to enrich for CD4 cells in a primary splenocyte culture. When used in tandem with a dead cell separation medium like Lympholyte-M, high ... Read Review
(1)

12/27/2019Pan CD4 T Cell Isolation Kit from Mouse Wei XuYou can use this kit to get acceptable yield and purity to isolate CD4 cells from mouse with reasonable price.

MojoSort(TM) Mouse CD4 T Cell Isolation Kit from BioLegend

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MojoSort(TM) Mouse CD4 T Cell Isolation Kit from BioLegend

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