Fig 1: Tree sublingual allergy immunotherapy tablet (SLIT-tablet) T-cell proliferation assay. Aliquots of the solubilized tree SLIT-tablet were collected at the time points indicated and combined with antigen-presenting cells (dendritic cells [DCs]) and Bet v-specific T-cells. T-cell proliferation was determined by CellTiter-Glo (CellTiter-Glo 2.0 (cat. no. G9241) Promega, Madison, Wisconsin) viability assay (triplicate, mean [SD]). DC + medium was included as a negative control. Figure shows representative data from 1 of 3 donors. NS = nonsignificant; RLU = relative light units. ⁎⁎⁎⁎P = 0.001
Fig 2: Apoptotic effect of GLUD1 siRNA. HepG2 and Human Hepatocytes were transfected with siRNA targeting human GLUD1 (siGLUD1) or control scramble siRNA (C). (A,B) Seventy-two hours later, cell viability was assessed by using Promega CellTiter-Glo® 2.0 Assay. (C) Twenty-four hours after GLUD1 gene silencing, HepG2 cells were assayed for caspase 3/7 and (D) stained with DAPI followed by visualization under fluorescence microscope (magnification 20×). Images in (D) are representative of three independent experiments with similar results. In (A–C), means ± S.D. of four replicate independent experiments are shown; differences between samples and relative controls are significant (# p < 0.05, Student’s t-test).
Supplier Page from Promega for CellTiter-Glo 2.0 Assay