Fig 1: Illustration of the steps involved inImmunohistochemistry and its implementation using an MFP.a Protocol for immunohistochemistry implemented with chromogen-based revelation. Rapid µIHC is enabled by implementing the three core steps of the IHC protocol using an MFP. The times shown for the individual steps of the conventional workflow are taken from the suggested protocol of the Mouse-specific HRP/DAB Detection Kit from Abcam (ab64259). b Scheme of three different reagents passing through the central aperture of an MFP head placed over a substrate. The central injection aperture is linked to three channels through which the reagents are injected sequentially. c The three core steps of rapid µIHC are (1) the binding of a primary antibody to an antigen on the sample, (2) the binding of a biotinylated secondary antibody to the primary antibody, and (3) the application of a streptavidin/peroxidase solution for visualization