Fig 1: Acute ischemic stroke patients exhibit increased cerebral microglia expressing high Netrin-1 and UNC5a. (A) Immunostaining for TMEM119 in brain tissue from patients who had died from acute ischemic stroke (AIS), as well as from age-matched normal cases (NC). (B) Quantification of the number of TMEM119-postive microglia in the brain. (C–F) Microglia were isolated from AIS and NC brain tissue using flow cytometry, for which microglia were determined and sorted out based on their co-expression for TMEM119 and CD45, shown by representative flow charts (C), and by quantification (D). (E, F) RT-qPCR (E) and ELISA (F) for Netrin-1 and its main receptors, which include DCC, UNC5a, UNC5b, UNC5d and A2B, in isolated microglia. *p<0.05. Scale bars were 100µm.