Fig 1: Supplementation with recombinant BMP2 alleviates PE-related phenotypes in Ad Flt1-induced PE rat model. a, Diagram illustrates the animal experimental protocol. Adenoviruses expressing sFlt-1 or control Fc were injected into the tail vein of Sprague–Dawley rats on G8. The pregnant rats then were subjected to tail vein recombinant BMP2 protein (10 µg/kg) or PBS injection daily from G10 to G13. Rats from 4 groups including Ad Fc + PBS (n = 3), Ad Fc + BMP2 (n = 3), Ad Flt1 + PBS (n = 3), and Ad Flt1 + BMP2 (n = 3) were harvested on G19 with fetal rats and tissues collection. Blood pressure was measured and recorded every three days from G7 to G19. Urine and blood samples were collected on G7, G13 and G19. b-c, Mean blood pressure (MBP) and systolic blood pressure (SBP) of pregnant rats from each group (n = 3) were analyzed. d, The representative image of fetal rats and placentas (G19) from each group (n = 7). e, HE staining of rat placentas was performed to observe placental labyrinth and junction areas. Representative images were presented in left panel and the placental labyrinth/junction ratios in each group were quantified and summarized in right panel (n = 9). Scale bar, 2 mm. f-g, The weight of fetal rats and corresponding placentas was determined on G19 in Ad Fc + PBS group (n = 46), Ad Fc + BMP2 group (n = 44), Ad Flt1 + PBS group (n = 45) and Ad Flt1 + BMP2 group (n = 44). Quantitative results are expressed as the mean with 95% CI and p values by two-way ANOVA are labeled.
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