Fig 1: Loss of microglial Olfml3 increased expression of alternate microglia-derived pro-angiogenic factors. (A) mRNA levels of Angpt2 were not affected by loss of microglial Olfml3 when cultured alone (p = 0.8582) or in the presence of ECs (48 h; p = 0.7947). (B) Fgf1 mRNA was increased in Olfml3-/- microglia cultured alone (p = 0.0243) but not in the presence of ECs (p = 0.5997). (C) Fgf2 mRNA levels were not affected by loss of Olfml3 in microglia cultured alone (p = 0.5139) but were moderately increased in the presence of ECs (p = 0.0407). (D) Hgf mRNA increased in Olfml3-/- microglia cultured alone (p = 0.0143) and in the presence of ECs. Notably, mRNA levels of VEGF family genes (E) Vegfa, (F) Vegfb, and (G) Vegfd were unchanged in Olfml3-/- microglia cultured alone or in the presence of ECs. (H) VEGF-A concentration, expressed as relative to isogenic control, in microglial supernatant was increased in Olfml3-/- microglia cultured alone and in the presence of ECs. Comparisons based on unpaired student’s t test; bars represent group mean with standard error of the mean (SEM).