Description
Based on extensive Design of Experiment (DOE) methodologies, the cationic lipid-based transfection reagent, lentiviral production supplement, and LV enhancer are specifically screened for maximal delivery of multiple DNA plasmids to achieve the highest possible lentiviral titer when used in conjunction with CTS Viral Production Cells cultured in CTS™ LV-MAX™ Production Medium.
All CTS™ LV-MAX™ Transfection Kit components are produced under cGMP manufacturing conditions with extensive quality-control testing to ensure proper preclinical, clinical, and commercial therapeutic viral vector applications.
CTS™ LV-MAX™ Transfection Kit features include:
• Uniquely designed to enhance transient transfection of multiple DNA plasmids into high density suspension HEK293-derived cell culture
• Enables high viral titers of up to 1 x 108 TU/mL (unconcentrated LVV-GFP) to allow for a cost-effective alternative to PEI-based transfection reagents
• Scalable protocol for 96-deep well blocks, flasks, and bioreactors
• Regulatory-compliant reagents produced in conformity with cGMP for medical devices, 21 CFR, Part 820, and certified to the ISO 13485. Drug Master File and Regulatory Support File available upon request.
The CTS™ LV-MAX™ Transfection Kit requires CTS Opti-MEM I (not included in this kit) for complexation. The chemically-defined, serum-free, animal origin-free features of the CTS™ LV-MAX™ Transfection Kit result in high reproducibility and lot-to-lot reliability. The formulation of this product is identical to the research-grade LV-MAX™ Transfection Kit, but the CTS™ LV-MAX™ Transfection Kit is validated for clinical and commercial therapeutic applications.
Custom configuration and packaging available upon request