Fig 1: Effect of AA and GA on SFRP4 level. The bars are representing the SFRP4 concentration in the cell lysate estimated by ELISA. (a) Ascorbic acid; (b) Gallic acid. Statistically significant differences are represented by asterisk (P < .05*, P < .01**, P < .001*** and P < .0001****).
Fig 2: Docking results of ascorbic acid with SFRP4 as receptor protein (a). AA displays interaction with Glu63 and His117 in the active pocket and Gln60, Tyr61, Leu64, Tyr115, Met112 are found in the surrounding region (left). Binding patterns of AA with SFRP4 (right). (b); Docking results of gallic acid with SFRP4. GA interacts with amino acid His117 and the amino acid residues Gln60, Glu63, Tyr61, Met112, and Pro120 are found in the surrounding region (left). Binding patterns of GA with SFRP4 (right).
Fig 3: Effect of AA and GA on serum SFRP4 level. The level of significance indicated by *(P < .05*, P < .01**, P < .001*** and P < .0001****). (a) HFD treatment significantly increased the level of serum SFRP4 (P < .0001). (b) AA treatment both AAL and AAH significantly decreased (P < .0001) the serum SFRP4 level.
Fig 4: Fold change in mRNA expression level of SFRP4: Relative mRNA expression in pancreas and adipose tissues of treated groups relative to diabetic control (NC: Normal Control; ST: Standard Treatment (Metformin); AAL: Ascorbic Acid Low, AAH: Ascorbic Acid High; GAL: Gallic Acid Low; GAH: Gallic Acid High). The bars are representing mean ± SD.
Supplier Page from Wuhan Fine Biotech Co., Ltd. for Mouse Sfrp4(Secreted frizzled-related protein 4) ELISA Kit